Reference

Creatine monohydrate supplemented in swine finishing diets and fresh pork quality: III

Creatine monohydrate supplemented in swine finishing diets and fresh pork quality: III. Evaluating the cumulative effect of creatine monohydrate and alpha-lipoic acid. Berg, E. P.; Maddock, K. R.; Linville, M. L. (Department of Animal Science, University of Missouri, Columbia 65211-5300, USA). Journal of Animal Science (Savoy, IL, United States), 81(10), 2469-2474 (English) 2003 American Society of Animal Science. CODEN: JANSAG. ISSN: 0021-8812. DOCUMENT TYPE: Journal CA Section: 18 (Animal Nutrition) Section cross-reference(s): 17 The effects of short-term supplementation with a-lipoic acid (ALA) and creatine monohydrate (CMH) to improve fresh pork quality were studied in 48 com. hybrid barrows given no CMH or ALA, 24 g CMH/pig/day, 600 mg ALA/pig/day, or CMH + ALA. The pigs were individually penned and had ad libitum access to water and finishing diet. The treatments were hand-fed to individual pigs daily in 3 equal doses for 5 days before slaughter at 113 kg body wt. The i.m. pH was recorded at 45 min postmortem and again at 24 h in the ham semimembranosus muscle (SM) and the longissimus muscle between the 10th and 11th rib (LM). A Meatcheck cond. probe was inserted in the same anatomical locations to provide an index value (PY) from 0 to 100 (higher index value indicated more intact muscle cells and higher water-holding capacity). Meat color values (L, a, b) were obtained at 24 h postmortem on the ham gluteus medius (GM), SM, and LM muscles. Two 2.54-cm thick loin chops were removed from the loin for the detn. of Warner-Bratzler shear force and glycolytic potential. The intact SM and the posterior portion of the boneless loin were vacuum-packaged and stored for 7 days to det. 50-21-5 and 6020-87-7 which are cas registry numbers of chemicals are mentioned. purge water loss. CMH-fed pigs had higher PY value in the SM (66.67) at 45 min postmortem than either ALA (63.50) or CMH + ALA (62.27) fed pigs. Lipoic acid led to the highest pH at 45 min. The results justify further study of the potential pos. influence of a-lipoic acid supplementation to improve pork quality. .

Histological assessment of intermediate- and long-term creatine monohydrate supplementation in mice and rats

Histological assessment of intermediate- and long-term creatine monohydrate supplementation in mice and rats. Tarnopolsky, M. A.; Bourgeois, J. M.; Snow, R.; Keys, S.; Roy, B. D.; Kwiecien, J. M.; Turnbull, J. (Departments of Pediatrics and Medicine (Rehabilitation), McMaster University, Hamilton, ON L8N 3Z5, Can.). American Journal of Physiology, 285(4, Pt. 2), R762-R769 (English) 2003 American Physiological Society. CODEN: AJPHAP. ISSN: 0002-9513. DOCUMENT TYPE: Journal CA Section: 18 (Animal Nutrition) Creatine monohydrate (CrM) supplementation is relatively safe based on data from short-term and intermediate-term human studies and several therapeutic trials. This study characterized pathol. changes after intermediate-term and long-term CrM supplementation in mice [healthy control and SOD1 (G93A) transgenic] and rats (prednisolone and nonprednisolone treated). Histol. assessment (18-20 organs/tissues) was performed in G93A mice after 159 days and in Sprague-Dawley rats after 365 days of CrM supplementation at 2% in chow feed compared with control feed. Liver histol. was also evaluated in CD-1 mice after 300 days of low-dose CrM supplementation (0.025 and 0.05 g/kg/day in drinking water) and in Sprague-Dawley rats after 52 days of 2% CrM supplementation in chow with and without prednisolone. 6020-87-7 is also in the experiment. Areas of hepatitis were obsd. in the livers of CrM-fed G93A mice, with no significant inflammatory lesions in any of the other 18-20 tissues/organs evaluated. The CD-1 mice also had hepatic inflammatory lesions, yet there was no neg. effect of CrM on liver histol. in the Sprague-Dawley rats after intermediate- or long-term supplementation nor was inflammation seen in any other tissues/organs. Thus, dietary CrM supplementation can induce inflammatory changes in the liver of mice, but not of rats. The obsd. inflammatory changes in the murine liver must be considered in the evaluation of hepatic metab. in CrM-supplemented mice. Species differences must be considered in toxicol. and physiol. studies. .