Cinolazepam (CAS NO.: ), with other name of 7-Chloro-1-(2-cyanoethyl)-5-(2-fluorophenyl)-3-hydroxy-1,3-dihydro-2H-1,4-benzodiazepin-2-one, could be produced through many synthetic methods.

Following is one of the synthesis routes:

7-Chloro-3-hydroxy-5-(2-fluorophenyl)-1,3-(dihydro-2H-1,4-benzodiazepin-2-one (I) is mixed with acrylonitrile (II), and after dropwise addition of triethylbenzylammonium chloride (A) and benzyl trimethylammonium hydroxide (B), stirring is continued for 8 hours. The crude material was recrystallized from acetonitrile.