EVIDENCE FOR CONVERSION OF ARACHIDONIC ACID TO HYDROXYICOSATETRAENOIC ACIDS BY A CELL-FREE HOMOGENATE FROM MAIZE SEEDLINGS

DOI: 10.1016/0031-9422(90)85165-C

Source and publish data:

Phytochemistry p. 2453 - 2455 (1990)

Update date:2022-09-26

Topics:: Authors:

Janistyn, Boris

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Article abstract of DOI:10.1016/0031-9422(90)85165-C

The conversion of arachidonic acid to the hydroxyicosatetraeonic acids 5-HETE, <5(S)-hydroxy-6,8,11,14-icosatetraeonic acid>, 12-HETE, <12(S)-hydroxy-5,8,10,14-icosatetraeonic acid> and 15-HETE, <15(S)-hydroxy-5,8,11,13-icosatetraenoic acid> by a 10000 g cell-free supernatant of a Zea mays seedlings homogenate is described.For metabolic pathway detection <1-14C>arachidonic acid was added.The hydroxyicosatetraenoic acids were isolated by TLC and HPLC and purified to constant specific radioactivity against authentic <3H>-labelled 5-HETE, 12-HETE and 15-HETE by co-chromatography.

Full text of DOI:10.1016/0031-9422(90)85165-C

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Article Doi

EVIDENCE FOR CONVERSION OF ARACHIDONIC ACID TO HYDROXYICOSATETRAENOIC ACIDS BY A CELL-FREE HOMOGENATE FROM MAIZE SEEDLINGS

DOI: 10.1016/0031-9422(90)85165-C

Source and publish data:

Authors:

Article abstract of DOI:10.1016/0031-9422(90)85165-C

Full text of DOI:10.1016/0031-9422(90)85165-C

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