Bacterial surface engineering utilizing glucosamine phosphate derivatives as cell wall precursor surrogates

Article abstract of DOI:10.1002/chem.200801734

A study was conducted to examine glucosamine phosphate derivatives as cell wall precursor surrogates for bacterial surface display. N-acetylglu-cosamine-1- phosphate (GlcNAc-1-phosphate) derivate was used in the study as a bacterial cell wall precursor. It was observed during the study that the acetylation of the hydroxyl groups with high hydrophobicity can provide easy access to the cytoplasm of the bacteria. A flow cytometry was used to examine the incorporation of the derivatives into the bacterial cell wall. The study also used acetate buffer and biotin-PEO-hydrazide for washing the lactic acid bacteria. The incubation of lactic acid bacteria was carried out under the anaerobic conditions. This study can be used for development of oral vaccines.