CONVERGENT SOLID PHASE PEPTIDE SYNTHESIS. IV. SYNTHESIS OF THE 35-43 AND 32-34 PROTECTED SEGMENTS OF THE TOXIN II OF ANDROCTONUS AUSTRALIS HECTOR.

Article abstract of DOI:10.1016/S0040-4020(01)82111-6

The protected peptide segments corresponding to the sequences 35-43 and 32-34 of toxin II of the scorpion Androctonus australis Hector have been synthesized on a p-alkoxybenzyl ester resin using the base-labile Fmoc α-amino protection and HF-labile side chain protecting groups.Crude peptides obtained after trifluoroacetic acid cleavage have been purified by solvent extraction, dimethylacetamide-water precipitation and semi-preparative reversed phase HPLC.Both synthetic and purification protocols have been optimized for good yields and high purity.Fast atom bombardment mass spectrometry has proved to be a very useful technique to characterize protected peptide segments.