Recueil des Travaux Chimiques des Pays-Bas p. 73 - 81 (1988)
Update date:2022-07-29
Topics:
Berg, E. M. M. van den
Baldew, A. U.
Goede, A. T. J. W. de
Raap, J.
Lugtenburg, J.
In this paper, we report the preparation of three isotopomers of L-tryptophan on a gram scale, specifically labelled with 99percent 15N (position 1) and 99percent 13C (position 2) in the indole ring; for position 3, the incorporation was 90percent 13C.The isotopically enriched indoles were synthesized from H(15)NO3, K(13)CN and (13)CH3CN and then converted, with L-serine, into the corresponding labelled L-tryptophans using genetic manipulated E. coli bacteria, which contain high amounts of the enzyme tryptophan synthetase.The 13C-13C, 13C-1H, 13C-15N and 15N-1H coupling constants of the indoles and the tryptophans were determined from the 1H NMR and 13C NMR spectra.
View MoreShanDong XinDa Chemical CO.,LTD(expird)
Contact:086-0311-87580543
Address:No.168, High Technology Development Zone Jinan Shandong China
Contact:+86-532-80762375
Address:No. 6, Hongkong Middle Road, Qingdao, China
Contact:0572-2722882
Address:1201,F3,xinghuibandao,
Jiangxi Dongxu Chemical Science & Technology Co.,Ltd
Contact:+86-791-86899381
Address:Nanchang, Jiangxi, China
Hangzhou Ocean Chemical Co., Ltd.
website:http://www.hzoceanchem.com
Contact:+86-571-88025872, 28272092, 28272096
Address:Room 623 ,Building No 1 , COFCO Radius Commercial Center Xiwen Road, Xiacheng District, Hangzhou, Zhejiang Province, China
Doi:10.1080/00397911.2016.1244271
(2016)Doi:10.1021/acs.langmuir.8b03129
(2019)Doi:10.1002/jhet.5570360133
(1999)Doi:10.1016/j.cclet.2019.07.012
(2019)Doi:10.1021/acs.joc.9b01046
(2019)Doi:10.1016/j.bmc.2014.12.029
(2015)
