REGIOSELECTIVE CLEAVAGE OF DI- AND POLY-RIBONUCLEOTIDES INDUCED BY CYCLODEXTRINS (CYCLOMALTO-OLIGOSACCHARIDES)

Article abstract of DOI:10.1016/0008-6215(89)85169-9

Cyclodextrins (cyclomalto-oligosaccharides, CDs) induce regioselective cleavage of the 3',5'-phosphodiester linkages in di- and poly-ribonucleotides at pH 11.08 and 50 deg C. 0.15M Cyclomaltohexaose (α-CD) mediates the cleavage of CpA, CpC, CpG, and CpU to give 96-97percent of cytidine 3'-phosphate together with adenosine, cytidine, guanosine, and uridine, respectively.In the absence of α-CD, 50-52percent of cytidine 2'-phosphate is formed as by-product. α-CD also promotes the formation of adenosine 3'-phosphate from ApA and ApC, guanosine 3'-phosphate from GpG, and uridine 3'-phosphate from UpU.In contrast, β-CD and γ-CD enhance the formation of the corresponding 2'-phosphates.Regioselective cleavages of poly, poly, poly, and poly are achieved also.