SPECIFITY OF E. COLI SHIKIMATE DEHYDROGENASE TOWARDS ANALOGUES OF 3-DEHYDROSHIKIMIC ACID

Article abstract of DOI:10.1016/S0040-4039(00)82453-3

Analogues of 3-dehydroshikimic acid which lack the C-4 and C-5 hydroxyl groups have been synthesised and assayed as substrates for shikimate dehydrogenase.The presence of the C-4 hydroxyl group is found to be very important for specificity, whereas the C-5 hydroxyl group is not.The enzyme exhibits enantioselectivity at C-1 and C-4 of the racemic substrate analogues.