400
D. Zhang / Spectrochimica Acta Part A 77 (2010) 397–401
peak at 10.06 ppm, which was the characteristic resonance of
aldehyde (–CHO), appeared. The 1H NMR of the isolated product
was identical with that of the standard compound 6, demon-
strating that 1 was deprotected by ClO− ions to give aldehyde
6. The determination of ClO− was based on this specific reac-
tion.
4. Conclusion
In conclusion, on the basis of a specific reaction for hypochlorite
and exploiting a ICT mechanism, we have successfully developed
two colorimetric probes, 1 and 2, which are highly sensitive and
specific for the detection of ClO−. Most importantly, the recognition
of ClO− gave obvious color changes from orange to pink, which
was clearly visible to the naked eyes. Moreover, the competition
experiments showed the interference from other common ions and
oxidants was minimal. Due to the simplicity and sensitivity of the
analysis, this sensor would have many opportunities in a variety of
settings requiring rapid and accurate ClO− analysis. We anticipate
that this probe will be of great benefit to biomedical researchers
for studying the effects of ClO− in biological systems.
Fig. 6. UV–vis spectra of probe 2 (10 M) in the absence and presence of 32 equiv.
of various species in phosphate buffer, pH 9.2/DMF (4:1, v/v).
Acknowledgment
The study was supported by the fundamental research funds for
the central universities (10D10510).
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