Synthesis of a trans, syn, trans-dodecahydrophenanthrene via a bicyclic transannular Diels-Alder reaction: Intermediate for the synthesis of fusidic acid

Article abstract of DOI:10.1021/jo101533h

While thermolysis of the macrobicyclic triene lactone 12 did not produce the expected bicyclic transannular Diels-Alder (BTADA) product 13, heating the corresponding ether 18 to 110 °C for 4 h afforded a quantitative yield of the desired cycloadduct 19, which could be easily reduced to the perhydrophenanthrene, an ABC ring analogue of fusidic acid 1. Theoretical calculations with hybrid density functional theory (B3LYP/6-31G(d)) help rationalize why the lactone does not cyclize whereas the ether does.

Full text of DOI:10.1021/jo101533h

pubs.acs.org/joc
Synthesis of a trans,syn,trans-Dodecahydrophenanthrene via a Bicyclic
Transannular Diels-Alder Reaction: Intermediate for the Synthesis
of Fusidic Acid
Michael E. Jung,* Ting-Hu Zhang, Rebecca M. Lui, Osvaldo Gutierrez,
and K. N. Houk*
Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E. Young
Drive, Los Angeles, California 90095-1569
jung@chem.ucla.edu; houk@chem.ucla.edu
Received August 4, 2010
While thermolysis of the macrobicyclic triene lactone 12 did not produce the expected bicyclic
transannular Diels-Alder (BTADA) product 13, heating the corresponding ether 18 to 110 °C for 4 h
afforded a quantitative yield of the desired cycloadduct 19, which could be easily reduced to the
perhydrophenanthrene, an ABC ring analogue of fusidic acid 1. Theoretical calculations with hybrid
density functional theory (B3LYP/6-31G(d)) help rationalize why the lactone does not cyclize
whereas the ether does.
Introduction
juncture stereochemistry correct, but with the C3-stereo-
chemistry inverted, in excellent yield and selectivity. We
wondered if a related bicyclic triene lacking the ketone
function could be induced to undergo a bicyclic trans-
annular Diels-Alder reaction (BTADA) to produce a simi-
lar trans,syn,trans-dodecahydrophenanthrene system, which
potentially could be used for the preparation of fusidic acid
and its analogues. We report here the results of those
exploratory studies.
Fusidic acid (1, Scheme 1) is a potent antibiotic used in a
variety of medical applications.1 Given its biological activity
and complex structure, several groups pursued its total
synthesis, with formal total syntheses being achieved by
Dauben and Tanabe years ago.2 Deslongchamps3 likewise
constructed a model ABC ring system using a monocyclic
transannular Diels-Alder (TADA)4 reaction of the trienone
diester 2, which gave 3 (Scheme 2), with the entire ABC ring
(4) For some recent TADA reactions, see, among others: (a) Takamura,
H.; Yamagami, Y.; Ito, T.; Ito, M.; Arimoto, H.; Kadota, I.; Uemura, D.
Heterocycles 2009, 77, 351–364. (b) Phoenix, S.; Reddy, M. S.; Deslong-
champs, P. J. Am. Chem. Soc. 2008, 130, 13989–13995. (c) Winbush, S. A. M.;
Mergott, D. J.; Roush, W. R. J. Org. Chem. 2008, 73, 1818–1829. (d) Anzo,
T.; Suzuki, A.; Sawamura, K.; Motozaki, T.; Hatta, M.; Takao, K.-i.;
Tadano, K.-i. Tetrahedron Lett. 2007, 48, 8442–8448. (e) Balskus, E. P.;
Jacobsen, E. N. Science 2007, 317, 1736–1740. (f) Felzmann, W.; Arion,
V. B.; Mieusset, J.-L.; Mulzer, J. Org. Lett. 2006, 8, 3849–3851. (g) Evans,
D. A.; Starr, J. T. J. Am. Chem. Soc. 2003, 125, 13531–13540. (h) Vosburg,
D. A.; Vanderwal, C. D.; Sorensen, E. J. J. Am. Chem. Soc. 2002, 124, 4552–
4553. (i) Shing, T. K. M.; Yang, J. J. Org. Chem. 1995, 60, 5785–5789. (j)
Takahashi, T.; Sakamoto, Y.; Doi, T. Tetrahedron Lett. 1992, 33, 3519–3522.
(k) For reviews of TADA, see: Vanderwal, C. D.; Sorensen, E. J. Strategies
Tactics Org. Synth. 2004, 5, 1–50. Marsault, E.; Toro, A.; Nowak, P.;
Deslongchamps, P. Tetrahedron 2001, 57, 4243–4260. Deslongchamps, P.
Aldrichim. Acta 1991, 24, 43–56.
(1) (a) Godtfredsen, W. O.; von Daehne, W.; Tybring, L.; Vangedal, S. J.
Med. Chem. 1966, 9, 15. (b) Riber, D.; Venkataramana, M.; Sanyal, S.;
Duvold, T. J. Med. Chem. 2006, 49, 1503. (c) Duvold, T.; Sorensen, M. D.;
Bjoerkling, F.; Henriksen, A. S.; Rastrup-Andersen, N. J. Med. Chem. 2001,
44, 3125. (d) Duvold, T.; Jorgensen, A.; Andersen, N. R.; Henriksen, A. S.;
Dahl Sorensen, M.; Bjorkling, F. Bioorg. Med. Chem. Lett. 2002, 12, 3569.
(2) (a) Dauben, W. G.; Kessel, C. R.; Kishi, M.; Somei, M.; Tada, M.;
Guillerm, D. J. Am. Chem. Soc. 1982, 104, 303. (b) Dauben, W. G.; Ahlgren,
G.; Leitereg, T. J.; Schwarzel, W. C.; Yoshioko, M. J. Am. Chem. Soc. 1972,
94, 8593. (c) Tanabe, M.; Yasuda, D. M.; Peters, R. H. Tetrahedron Lett.
1977, 1481. (d) Ireland, R. E.; Beslin, P.; Giger, R.; Hengartner, U.; Kirst,
H. A.; Maag, H. J. Org. Chem. 1977, 42, 1267. (e) Ireland, R. E.; Giger, R.;
Kamata, S. J. Org. Chem. 1977, 42, 1276.
(3) Fortin, S.; Barriault, L.; Dory, Y. L.; Deslongchamps, P. J. Am.
Chem. Soc. 2001, 123, 8210. For similar work, see: Germain, J.; Deslong-
champs, P. J. Org. Chem. 2002, 67, 5269–5278.
DOI: 10.1021/jo101533h
r
Published on Web 09/15/2010
J. Org. Chem. 2010, 75, 6933–6940 6933
2010 American Chemical Society

Jung et al.
JOCArticle
SCHEME 1. Structure of Fusidic Acid 1
NOESY. We elected to carry out the BTADA of the lactone
12 rather than that of the silyloxytriene ester 11 for two
reasons: (a) the lactone would have fewer steric interactions
than the silyl ether ester because the ester and hydroxyl group
would be connected and (b) the lactone 12 would hold the
triene system in the specific conformation 12A (Scheme 4) so
that only the trans,syn,trans Diels-Alder adduct 12B could be
formed. Although Deslongchamps had shown in his mono-
cyclic system that this was the preferred mode of cyclization,3
closer examination of molecular models revealed that this
might well not be the case here. In fact, it seemed likely that
the monocyclic silyloxy triene ester would prefer to cyclize via
the conformation 11A, which would give the cis,syn,cis
Diels-Alder adduct 11B. Thus the TBDPS ether of 11 was
removed with TBAF (65% yield) and the ester was hydrolyzed
by using lithium hydroxide in methanol to give the hydroxy
acid in 70% yield. Cyclization of this hydroxy acid was carried
out with the Yamaguchi reagent to give the bicyclic lactone 12
in 61% yield. Thus the triene ester 11 was available in 14 steps
and 20% overall yield from 4 with the lactone 12 being
available in three more steps and overall 5.5% yield from 4.
Withthe triene lactone12 in hand, weattempted its BTADA
reaction. Heating 12 at different temperatures and in various
solvents gave no products with the desired dodecahydrophe-
nanthrene skeleton (Scheme 5). Starting material was con-
sumed but many products were formed, none of which had the
characteristics of the desired Diels-Alder product. Examina-
tion of molecular models indicated that the triene lactone
could adopt an appropriate conformation favorable for the
BTADA and therefore it was disappointing that the desired
cycloaddition did not occur. However, manipulation of the
molecular models indicated that there was also another readily
accessible conformation that was not in position to give the
cycloadduct. For this reason, we decided to look at two
alternate strategies: (a) preparation and cyclization of an
alternate substrate and (b) theoretical calculations6 to better
understand the conformational energy landscape of these
substrates with the aim of carrying out the desired BTADA.
Of the many possible analogues of the lactone 12 which
might have a more favorable equilibrium for the conforma-
tion leading to cyclization, we first chose compounds with
more atoms in the connecting chain. This was in the hope
that the higher conformational flexibility would allow the
molecule to attain the required transition state conformation
for the cycloaddition. The ester 11 was reduced with DIBAL
and the silyl ether cleaved to give, in 78% yield for the two
steps, the diol 14, which was converted into the cyclic bis-
(silyl) ether 15 in 68% yield (Scheme 6). Heating 15 in a
microwave at 125 °C for 10 h afforded a 75% yield of the
product of a [1,5]-hydride shift,7 namely the silyl enol ether
16 instead of the desired cycloadduct 17. Therefore the bis-
(silyl) ether 15 preferred a conformation such as E in which the
[1,5]-hydride shift was more favorable than the desired
Diels-Alder cycloaddition.
SCHEME 2. TADA for the Synthesis of trans,syn,trans-Dode-
cahydrophenanthrene
Results and Discussion
The synthesis of the bicyclic triene lactone 12, with the
carbon precursor to the methyl group at C-8 and the required
alcohol at C-11 (Scheme 3), was straightforward and generally
high-yielding. The known E-enoate5 4, readily available from
5-bromo-1-pentanol via oxidation to the aldehyde and Wittig
reaction, was reduced with DIBAL to give in 94% yield the
primary alcohol that was then protected to produce the TBS
ether 5 in quantitative yield. SN2 displacement of the bromide
with the commercially available lithium acetylide ethylenedia-
mine (EDA) complex gave, in 94% yield, the terminal alkyne,
the anion of which underwent carboalkoxylation to afford the
acetylenic ester 6. Addition of lithium dimethylcuprate to 6
provided, in 94% yield, the desired Z stereoisomer of the
β-methylacrylate, thereby setting the required stereochemistry
of the olefin that is destined to be the syn related proton and
methyl groups at C9-C10. DIBAL reduction of this ester gave
the Z-allylic alcohol 7 in quantitative yield. The aldehyde
formed by Dess-Martin periodinane (DMP) oxidation of this
alcohol (91% yield) was reacted with the 3-alkoxypropyl
Grignard reagent to give the expected alcohol in 87% yield.
Final protection of the hydroxyl function, which will become
the 11-hydroxy group of fusidic acid, with TBDPS chloride
furnished the triether 8 in 94% yield. The p-methoxybenzyl
(PMB) protecting group was removed in 80% yield with DDQ
and the alcohol was converted to the bromide 9 in 90% yield.
Alkylation of 9 with the anion of methyl bis(trifluoroethyl-
)phosphonoacetate (sodium hydride in DMF) furnished the
desired phosphono ester in 70% yield. Removal of the TBS
ether with aqueous acid (96% yield) and oxidation with
Dess-Martin periodinane furnished the aldehyde 10, which
was not purified but immediately subjected to an intramole-
cular Horner Wadsworth Emmons reaction to give the desired
macrocyclic enoate 8 in 90% yield for the two steps. The
stereochemistry of the new alkene was shown to be mainly the
desired Z-isomer by several NMR experiments, especially
Theoretical calculations were carried out which showed
that although the conformation A0 did indeed place the diene
(6) All calculations were carried out with B3LYP-6-31G(d) as implemen-
ted in Gaussian 03. Frisch, M. J. et al. Gaussian03, Revision C.02; Gaussian,
Inc., Wallingford, CT, 2004.
(7) Miller, B. Advanced Organic Chemistry, 2nd ed.; Prentice Hall: Upper
ꢀ
Saddle River, NJ, 2004. Garcia-Granados, A.; Lopez, P. E.; Melguizo, E.;
Parra, A.; Simeo, Y. Tetrahedron 2004, 60, 1491.
(5) Fischer, C.; Smith, S. W.; Powell, D. A.; Fu, G. C. J. Am. Chem. Soc.
2006, 128, 1472–1473.
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Jung et al.
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SCHEME 3. Synthesis of Bicyclic Triene Lactone 12, BTADA Substrate
SCHEME 4. Conformational Analysis of the Silyloxy Ester 11
and the Lactone 12
SCHEME 6. Attempted BTADA Reaction of the Bicyclic Bis-
(silyl) Ether 15
SCHEME 5. Attempted BTADA Reaction of the Bicyclic
Triene Lactone 12
energy needed for the diene to rotate out of conjugation as well
as the increase in transannular strain as the CdO bond is
inverting. Thus it was obvious that the lactone was too strained
to easily convert the most stable conformation A into the
conformer A0 that could undergo the cycloaddition. Significant
changes in the macrobicyclic skeleton are required for this
conformational change, compounded by the necessity to rotate
about the ester linkage. As a result, several analogues were
studied computationally, the most promising of which ap-
peared to be the simple ether, where the lactone CO is replaced
by a CH2 group. Conformation B0 was 10.3 kcal/mol higher in
energy than B but the calculated barrier for their interconver-
sion via B-TS-1 is only 15.1 kcal/mol. This molecule B is
predicted to have an activation free energy for cyclization of
only 26.2 kcal/mol, with a ready interconversion of confor-
mers, and thus cyclization to B00 (19) is quite favorable.
and dienophile in the proper orientation for the cyclo-
addition to 13, this was not the most stable conformation
(Figure 1). Conformation A0 was 7.4 kcal/mol higher in
energy than A; the calculated barrier for their interconversion
via A-TS-1 is 51.3 kcal/mol. The high barrier for interconver-
sion is attributed to a combination of factors including the
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FIGURE 1. Theoretical calculations of the BTADA transitions states. All structures were computed with B3LYP/6-31G(d). Gibbs free
energies are in kcal/mol. Selected distances are in angstroms.
Thus when the diol 14 was treated with triphosgene,
instead of the carbonate, the cyclic ether 18 was formed in
65% yield (Scheme 7). Presumably the primary allylic alco-
hol is converted to the allylic chloride, which cyclizes with the
secondary alcohol. This same ether could also be formed by
treatment of the diol 14 with thionyl chloride although in
somewhat lower yield. Heating 18 for 4 h at 110 °C afforded
the cycloadduct 19 as the sole product isolated in quantita-
tive yield. In fact, during the formation of 18 from 14 at
25 °C, we had already isolated about 20% of the cycloadduct
19, thus demonstrating the ease of this BTADA reaction.
The structure of 19 was assigned based on the proton and
carbon NMR data, which indicated that the desired cycload-
dition had indeed occurred. However the stereochemistry of
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Jung et al.
JOCArticle
distilled from calcium hydride under an argon atmosphere.
Dimethyl sulfoxide (DMSO) was distilled from calcium hydride
˚
and stored over 4 A molecular sieves. All other solvents or
reagents were purified according to literature procedures. 1H
NMR and 13C NMR spectra were obtained at 400 and 500
MHz for proton and 100 and 125 MHz for carbon. The chemical
shifts are reported in parts per million (ppm, δ). The coupling
constants are reported in Hertz (Hz) and the multiplicities are
reported as follows: br (broad), s (singlet), d (double), t (triplet), q
(quartet), and m (multiplet). Thin-layer chromatography (TLC)
was carried out with precoated silica gel sheets. Visual detection
was performed with ultraviolet light, p-anisaldehyde stain, potas-
sium permanganate stain, or iodine. Flash chromatography was
performed with flash grade silica gel with compressed air.
(E)-7-Bromohept-2-en-1-ol. To a stirred solution of 2.35 g
(10 mmol) of (E)-ethyl 7-bromohept-2-enoate5 (4) in 25 mL of
THF was added 25 mL of DIBAL-H (1.0 M in hexane) at
-78 °C under Ar. The solution was stirred at -78 °C for 1 h and
then was gradually warmed to -30 °C. TLC showed the reaction
completed. A saturated K-Na tartrate solution (3.0 mL) was
added slowly to the solution until no hydrogen was generated.
The solution was warmed to 21 °C and 20.0 mL of a satd K-Na
tartrate solution and 30.0 mL of ether were added. The solution
was stirred at 21 °C until it became clear. The aqueous phase was
extracted twice with ether and the combined organic phase was
washed with water and brine and dried over MgSO4. Removal
of the solvent afforded 1.74 g (91%) of the known crude
alcohol,8 which was pure enough for the next reaction. 1H
NMR (500 MHz, CDCl3) δ 5.70 (m, 2H), 4.18 (d, J = 5.5 Hz,
2H), 3.47 (t, J = 6.5 Hz, 2H), 2.13 (m, 2H), 1.93 (m, 2H), 1.59
(m, 2H); 13C NMR (125 MHz, CDCl3) δ 132.2, 129.5, 63.5, 33.5,
32.0, 31.1, 27.5.
FIGURE 2. Structure of 19.
SCHEME 7. BTADA Reaction of the Bicyclic Triene Ether 18
(E)-7-Bromo-1-[(1,1-dimethylethyl)dimethylsilyloxy]hept-2-ene,
5. To a stirred solution of 3.5 g (18.2 mmol) of the above alcohol in
30 mL of THF was added 3.06 g (45.5 mmol, 2.5 equiv) of
imidazole and 4.10 g (27.0 mmol, 1.5 equiv) of TBSCl. The
solution was stirred at 21 °C for 3 h. The solution was cooled to
0 °C and quenched with satd NaHCO3 (20.0 mL) and the mixture
was allowed to warm to 21 °C. After the mixture had stirred for
30 min at 21 °C, 40 mL of ether was added and the phases were
separated. The aqueous phase was extracted twice with 10 mL of
ether and the combined organic layer was washed with water and
brine and dried over MgSO4. Removal of the solvent provided a
crude oil that was evacuated under high vacuum overnight to give
the structure was established by a single-crystal X-ray struc-
ture (Figure 2), which unambiguously shows the trans,syn,
trans nature of the dodecahydrophenanthrene system. As
expected, this ring structure requires that ring B be held in a
boat conformation. The Diels-Alder adduct 19 could be
easily converted into an analogue of the ABC ring system of
fusidic acid by catalytic hydrogenation to give 20. Further-
more, the compound has the correct stereochemistry and
convertible functional groups at all ring junctions of the
ABC ring subunit of fusidic acid.
In summary, we have demonstrated that the trans,syn,
trans-perhydrophenanthrene ring system can be prepared
easily by a bicyclic transannular Diels-Alder (BTADA)
reaction of the properly arranged bicyclic triene ether 18 to
produce, after processing, the desired system 20. The use of
such a process for the synthesis of fusidic acid and other
natural terpenoids having ring B locked in the boat con-
formation is currently under study in our laboratories.
1
5.8 g (100%) of the bromo silyl ether. H NMR (500 MHz,
CDCl3) δ 5.58 (m, 2H), 4.12 (dd, J = 6.0, 1.3 Hz, 2H), 3.40 (t, J =
8.5 Hz, 2H), 2.06 (td, J = 8.5, 8.5 Hz, 2H), 1.85 (m, 2H), 1.52 (m,
2H), 0.90 (s, 9H), 0.06 (s, 6H); 13C NMR (125 MHz, CDCl3)
δ 130.3, 129.9, 63.9, 33.8, 32.2, 31.3, 27.7, 26.0, 18.5, -5.1.
(E)-9-[(1,1-Dimethylethyl)dimethylsilyloxy]non-7-en-1-yne. To
a stirred solution of 600 mg of lithium acetylide ethlyenediamine
(6.5 mmol, 2 equiv) in 8.0 mL of dry DMSO in a 20 mL vial under
Ar was added slowly 1.0 g (3.25 mmol) of the bromide, 5. The
solution was stirred at 21 °C for 40 min and was then poured into a
solution of 20 mL of ether and 10 mL of water. The aqueous layer
was extracted twice with 10 mL of ether and the combined organic
layer was washed with water and brine and dried over MgSO4.
Removal of the solvent and purification by flash column chro-
matography on silica gel with hexane:ethyl acetate (6:1) afforded
770 mg (94%) of the alkyne. 1H NMR (500 MHz, CDCl3) δ 5.62
(m, 2H), 4.17 (d, J = 5.5 Hz, 2H), 2.23 (m, 2H), 2.09 (m, 2H), 1.98
(t, J = 2.7 Hz, 1H), 1.56 (m, 4H), 0.96 (s, 9H), 0.11 (s, 6H); 13
C
Experimental Section
NMR (125 MHz, CDCl3) δ 130.7, 129.4, 84.5, 68.0, 62.9, 32.1,
28.2, 25.9, 24.9, 18.3, 18.2, -5.4. HRMS calcd for C15H32NOSi
General. All reactions were carried out under an argon atmo-
sphere unless otherwise specified. Tetrahydrofuran (THF) and
diethyl ether were distilled from benzoquinone ketyl radical under
an argon atmosphere. Dichloromethane, toluene, benzene, pyr-
idine, triethylamine, and diisopropylethylamine (DIPEA) were
(8) Fernandez-Garcia, C.; McKervey, M. A. Tetrahedron: Asymmetry
1995, 6, 2905–2906.
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Jung et al.
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[M þ NH4]þ 270.2253, found (GCMS) m/z 270.2249, error
washed with 10 mL of satd Na2S2O3 twice and brine and dried over
MgSO4. Purification by flash column chromatography on silica gel
with hexane:ethyl acetate (3:1) afforded 544 mg (91%) of the pure
enal. 1H NMR (500 MHz, CDCl3) δ 10.00 (d, J = 8.0 Hz, 1H),
5.91 (d, J = 8.2 Hz, 1H), 5.60 (m, 2H), 4.16 (dd, J = 6.2, 1.2 Hz,
2H), 2.57 (t, J = 9.5 Hz, 2H), 2.06 (m, 2H), 1.96 (d, J = 1.5 Hz,
3H), 1.55 (m, 2H), 1.43 (m, 2H), 0.90 (s, 9H), 0.06 (s, 6H); 13C
NMR (125 MHz, CDCl3) δ 192.5, 164.4, 130.2, 129.7, 128.3, 63.8,
32.3, 31.7, 28.8, 28.1, 25.8, 24.9, 18.3, -5.2.
(2Z,8E)-13-(4-Methoxyphenylmethoxy)-8-methyl-1-[(1,1-dimethy-
lethyl)dimethylsilyloxy]deca-2,8-dien-10-ol. A solution of 2.9 g (11.0
mmol, 2.5 equiv) of 1-bromo-3-(4-methoxyphenylmethoxy)-
propane in 25 mL of THF was added to 5.0 g of Mg and I2 was
used as the initiator. After the solution refluxed for 2 h, it was cooled
to 21 °C. The above solution was transferred by syringe to a
solution of 1.3 g (4.4 mmol) of the above enal in 15 mL of THF
at -78 °C. The resulting solution was allowed to stir for 1 h and was
then warmed to 21 °C. After being stirred for 1 h at 21 °C, the
reaction was quenched with satd NH4Cl. The organic phase was
washed twice with 10 mL of satd NH4Cl, water, and brine. After
drying over MgSO4, the solvent was removed to afford 1.80 g
(87%) of the allylic alcohol. 1H NMR (500 MHz, CDCl3) δ7.25 (d,
J = 8.5 Hz, 2H), 6.88 (d, J = 8.5 Hz, 2H), 5.61 (m, 1H), 5.55 (m,
1H), 5.17 (d, J = 9.5 Hz, 1H), 4.44 (s, 2H), 4.35 (m, 1H), 4.11 (d,
J= 5.5 Hz, 2H), 3.80 (s, 3H), 3.47 (t, J= 6.0 Hz, 2H), 2.03 (m, 4H),
1.68 (s, 3H), 1.7-1.3 (m, 8H), 0.90 (s, 9H), 0.06 (s, 6H); 13C NMR
(125 MHz, CDCl3) δ 159.1, 138.9, 131.0, 130.3, 129.3, 129.2, 128.3,
113.7, 72.6, 70.1, 67.9, 63.9, 55.2, 34.9, 32.0, 31.9, 29.0, 27.7, 26.0,
25.9, 23.3, 18.3, -5.2.
(2E,8Z)-13-(4-Methoxyphenylmethoxy)-8-methyl-1-[(1,1-dimethy-
lethyl)dimethylsilyloxy]-10-[(1,1-dimethylethyl)diphenylsilyloxy]-
trideca-2,8-diene, 8. To a stirred solution of 600 mg (1.26 mmol)
of the above allylic alcohol in 15 mL of dry DMF was added
300 mg (4.41 mmol, 3.5 equiv) of imidazole and 518 μL (1.89 mmol,
1.5 equiv) of TBDPSCl. The solution was allowed to stir at 21 °C
overnight and then was quenched with 5% NaHCO3. After addi-
tion of 30 mL of ether, the solution was stirred at 21 °C for 30 min.
After extraction and washing, the solution was dried over MgSO4.
Purification by flash column chromatography on silica gel with
hexane:ethyl acetate (6:1) gave 847 mg (94%) of the silyl ether. 1H
NMR (500 MHz, CDCl3) δ7.65 (m, 4H), 7.35 (m, 6H), 7.22 (d, J=
8.5 Hz, 2H), 6.86 (d, J = 8.5 Hz, 2H), 5.48 (m, 2H), 5.13 (d, J =
8.5 Hz, 1H), 4.36 (s, 2H), 4.35 (m, 1H), 4.09 (d, J = 5.5 Hz, 2H),
3.80 (s, 3H), 3.34 (t, J = 6.5 Hz, 2H), 1.82 (m, 2H), 1.70-1.30 (m,
9H), 1.03 (m, 4H), 1.02 (s, 9H), 0.91 (s, 9H), 0.09 (s, 6H); 13C NMR
(125 MHz, CDCl3) δ 159.0, 136.0, 135.9, 135.8, 134.7, 134.5, 134.4,
131.1, 130.7, 129.3, 129.11, 129.06, 129.04, 128.6, 127.3, 127.1,
113.6, 72.3, 70.2, 69.7, 63.9, 55.2, 35.2, 31.8, 31.7, 29.1, 27.2, 26.9,
25.9, 25.2, 22.9, 19.2, 18.3, -5.2.
0.4 ppm.
(E)-Ethyl 10-[(1,1-Dimethylethyl)dimethylsilyloxy]dec-8-en-
2-ynoate, 6. To a stirred solution of 900 mg (3.57 mmol) of the
above terminal alkyne in 10 mL of THF at -78 °C was added
dropwise 2.45 mL of tert-butyllithium (1.6 M in hexane,
3.92 mmol, 1.1 equiv). The solution was stirred at -78 °C for
10 min and then was warmed to -10 °C. The reaction mixture
was cooled again to -78 °C and 407 μL (4.28 mmol, 1.2 equiv) of
ethyl chloroformate was added. The resulting solution was
stirred at -78 °C for 1 h and was then warmed slowly to 0 °C.
After being stirring for 1 h, the reaction mixture was quenched
with 5 mL of saturated NH4Cl, followed by the addition of
30 mL of ether. The organic phase was washed with water and
brine and dried over MgSO4. Purification by flash column
chromatography on silica gel with hexane:ethyl acetate (5:1)
1
afforded 855 mg (74%) of the ester 6. H NMR (500 MHz,
CDCl3) δ 5.59 (m, 2H), 4.21 (q, J = 7.0 Hz, 2H), 4.12 (dd, J =
5.0, 1.0 Hz, 2H), 2.33 (t, J = 7.0 Hz, 2H), 2.04 (m, 2H), 1.59 (m,
2H), 1.49 (m, 2H), 1.30 (t, J = 7.0 Hz, 3H), 0.90 (s, 9H), 0.07 (s,
6H); 13C NMR (125 MHz, CDCl3) δ 154.9, 130.3, 129.7, 89.1,
73.1, 63.8, 61.7, 31.4, 28.2, 26.9, 25.9, 18.4, 18.3, 13.9, -5.2.
(2Z,8E)-Ethyl 3-Methyl-10-[(1,1-dimethylethyl)dimethylsilyloxy]-
deca-2,8-dienoate. To a stirred suspension of 402 mg (2.11 mmol,
1.2 equiv) of CuI in 20 mL of THF at -10 °C was added 2.64 mL
of methyllithium (1.6 M in hexane, 4.22 mmol). The solution was
allowed to stir at 0 °C. When the solution became clear, it was
cooled to -78 °C and then a solution of 570 mg (1.76 mmol) of the
ynoate 6 in 4 mL of THF was added. The reaction mixture was
stirred at -78 °C for 30 min and then warmed to -40 °C. After
being stirred for 30 min, the solution was poured into a solution of
30 mL of ether and 10 mL of satd NH4Cl. The organic layer was
washed with satd NH4Cl, water, and brine and dried over MgSO4.
Removal of the solvent gave 560 mg (94%) of the Z-acrylate,
1
which was used directly in the next step. H NMR (500 MHz,
CDCl3) δ 5.69 (s, 1H), 5.66 (m, 1H), 5.59 (m, 1H), 4.17 (m, 4H),
2.67 (t, J = 7.5 Hz, 2H), 2.09 (m, 2H), 1.91 (d, J = 1.5 Hz, 3H),
1.50 (m, 4H), 1.31 (t, J = 7.0 Hz, 3H), 0.95 (s, 9H), 0.10 (s, 6H);
13C NMR (125 MHz, CDCl3) δ 166.3, 160.4, 131.0, 129.2, 116.0,
63.9, 59.3, 33.0, 31.9, 29.1, 27.6, 25.9, 25.0, 18.3, 14.2, -5.2.
(2Z,8E)-3-Methyl-10-[(1,1-dimethylethyl)dimethylsilyloxy]-
deca-2,8-dien-1-ol, 7. Toa stirredsolution of2.2 g (6.47mmol)of
the aboveester in20 mL ofTHF was added16.5mL ofDIBAL-H
(1.0 M in hexane, 2.5 equiv). The solution was stirred at -78 °C
for 1 h and then was warmed slowly to -40 °C. After being stirred
at -40 °C for 30 min, the reaction was quenched with satd K-Na
tartrate(10mL). After the addition of 40 mLofether, the solution
was allowed to stir at 21 °C until it became clear. The organic
layer was washed with water and brine and dried over MgSO4.
Removal of the solvent gave 2.01 g (100%) of the crude allylic
alcohol 7, which was pure enough for the next reaction. 1H NMR
(500 MHz, CDCl3) δ 5.60 (m, 1H), 5.54 (m, 1H), 5.41 (t, J = 8.5
Hz, 1H), 4.12 (d, J = 5.5 Hz, 2H), 4.11 (d, J = 5.0 Hz, 2H), 2.06
(m, 4H), 1.72 (d, J = 1.0 Hz, 3H), 1.37 (m, 4H), 0.89 (s. 9H), 0.06
(s, 6H); 13C NMR (125 MHz, CDCl3) δ 140.3, 131.0, 129.3, 123.9,
63.9, 59.0, 31.9, 31.6, 28.8, 27.6, 25.9, 23.3, 18.3, -5.2. HRMS
calcd for C17H34O2Si 321.2226, found (ESI) m/z 321.2232, error
0.6 ppm.
(2Z,8E)-3-Methyl-10-[(1,1-dimethylethyl)dimethylsilyloxy]deca-
2,8-dienal. To a solution of 600 mg (2.01 mmol) of the above allylic
alcohol in 10 mL of dichloromethane was added sequentially
320 mg (3.81 mmol, 1.9 equiv) of NaHCO3 and then 1.5 g (3.6
mmol, 1.8 equiv) of Dess-Martin periodinane (DMP). The solu-
tion was stirred at 21 °C for 2 h. When TLC indicated no allylic
alcohol remaining, 10 mL of satd NaHCO3 was added, which was
then followed by the addition of 10 mL of satd Na2S2O3. The
resulting solution was stirred for 20 min and then the phases were
separated by the addition of 30 mL of ether. The organic phase was
(5Z,11E)-6-Methyl-13-[(1,1-dimethylethyl)dimethylsilyloxy]-
4-[(1,1-dimethylethyl)diphenylsilyloxy]trideca-5,11-dien-1-ol. To a
stirred solution of 550 mg (0.77 mmol) of the PMB ether 8 in
16 mL of dichloromethane and 4 mL of a pH 7 buffer solution was
added 262 mg (1.16 mmol, 1.5 equiv) of DDQ in five portions.
After the addition, the solution was stirred at 0 °C for 1 h and then
was warmed to 21 °C. The solution was kept at 21 °C until TLC
indicated no starting material remaining. The reaction was then
quenched with satd NaHCO3 and was extracted twice with 20 mL
of ether. The organic phase was washed with water and brine and
dried over MgSO4. Removal of the solvent gave a crude yellow oil
that was then dissolved again in 15 mL of methanol. To this
solution was added 73.0 mg (1.92 mmol, 2.5 equiv) of NaBH4 at
0 °C. After being stirred at 21 °C for 1 h, the solution was added to
20 mL of brine and 40 mL of ether. The layers were separated and
the organic phase was washed with brine and dried over MgSO4.
The residue after removal of the solvent was purified by flash
column chromatography on silica gel with hexane:ethyl acetate
1
(3:1) as eluent to give 366 mg (80%) of the primary alcohol. H
6938 J. Org. Chem. Vol. 75, No. 20, 2010

Jung et al.
JOCArticle
NMR (500 MHz, CDCl3) δ 7.66 (t, J = 6.5 Hz, 4H), 7.40 (m, 6H),
5.50 (m, 2H), 5.20 (d, J = 9.0 Hz, 1H), 4.40 (m, 1H), 4.08 (d, J =
5.0 Hz, 2H), 3.54 (m, 2H), 1.84 (m, 2H), 1.50 (s, 3H), 1.70-1.00 (m,
10H), 1.03 (s, 9H), 0.90 (s, 9H), 0.07 (s, 6H); 13C NMR (125 MHz,
CDCl3) δ 136.2, 135.9, 135.8, 134.7, 134.3, 134.2, 131.1, 129.5,
129.4, 129.2, 129.0, 128.3, 127.6, 127.4, 127.1, 69.7, 63.9, 63.0, 34.9,
31.8, 29.0, 28.2, 27.1, 26.9, 26.4, 25.9, 22.9, 19.2, 18.4, -5.2.
(2E,8E)-13-Bromo-8-methyl-1-[(1,1-dimethylethyl)dimethylsily-
loxy]-10-[(1,1-dimethylethyl)diphenylsilyloxy]deca-2,8-diene, 9. To
a stirred solution of the above primary alcohol (500 mg, 0.84
mmol) in dichloromethane (20 mL) was added triethylamine
(1.16 mL, 8.4 mmol, 10 equiv), followed by triphenylphosphine
(650 mg, 2.47 mmol, 3 equiv) and carbon tetrabromide (824 mg,
2.47 mmol, 3 equiv). After being stirred for 1 h, the reaction was
quenched with satd NaHCO3 and extracted twice with dichlor-
omethane. The combined extracts were dried over MgSO4, filtered,
and concentrated in vacuo. Flash chromatography with hexane:
ethyl acetate (10:1) as eluent afforded 497 mg (90%) of the bromide
9. 1H NMR (500 MHz, CDCl3) δ 7.65 (t, J = 8.0 Hz, 4H), 7.39 (m,
6H), 5.56 (m, 2H), 5.20 (d, J = 3.5 Hz, 1H), 4.41 (m, 1H), 4.15 (dd,
J = 5.5, 1.0 Hz, 2H), 3.29 (dt, J = 7.0, 1.0 Hz, 2H), 1.85 (m, 4H),
1.52 (d, J = 1.5 Hz, 3H), 1.70-1.00 (m, 8H), 1.08 (s, 9H), 0.90 (s,
9H), 0.06 (s, 6H); 13C NMR (125 MHz, CDCl3) δ 136.4, 135.9,
135.7, 134.3, 134.2, 131.0, 129.4, 129.2, 129.1, 128.3, 127.4, 127.1,
69.0, 63.9, 37.1, 34.0, 31.8, 31.7, 29.1, 28.4, 27.2, 26.9, 25.9, 22.9,
19.2, 18.3, -5.2; HRMS calcd for C36H57Br79O2Si2 679.2978,
found (ESI) m/z 679.2978, error 0.3 ppm, calcd for C36H57Br79O2-
Si2 681.2965, found (ESI) m/z 681.2968, error 0.3 ppm.
(7Z,13E)-Methyl 2-Bis(2,2,2-trifluoroethoxy)phosphoryl-8-
methyl-15-[(1,1-dimethylethyl)dimethylsilyloxy]-6-[(1,1-dimethy-
lethyl)diphenylsilyloxy]pentadeca-7,13-dienoate. To a stirred sus-
pension of NaH (60%, 84.0 mg, 2.1 mmol, 2.5 equiv) in anhydrous
DMF (2.5 mL) was added methyl 2-(bis(2,2,2-trifluoroethoxy)-
phosphoryl)acetate (670 mg, 2.1 mmol, 2.5 equiv). The resulting
solution was stirred at 21 °C for 1.5 h and a solution of the bromide
9(540 mg, 0.82 mmol) was added. The solution was heated to 60 °C
overnight and then was partitioned between ether (20 mL) and
water (10 mL). The reaction mixture was extracted with ethyl
acetate and the organic phase was washed with water and brine and
dried over MgSO4. Removal of the solvent gave a residue that was
purified by flash column chromatography on silica gel (hexane:
ethyl acetate 2:1) to afford 513 mg (70%) of the phosphono ester.
1H NMR (500 MHz, CDCl3) δ7.64 (m, 4H), 7.40 (m, 6H), 5.56 (m,
2H), 5.17 (t, J = 8.0 Hz, 1H), 4.41 (m, 5H), 4.14 (d, J = 5.0 Hz,
2H), 3.78 (d, J = 9.5 Hz, 3H), 3.10 (m, 1H), 1.98 (m, 1H), 1.88 (t,
J = 6.5 Hz, 2H), 1.70-1.10 (m, 14H), 1.06 (s, 9H), 0.95 (s, 1H),
0.10 (s, 6H); 13C NMR (125 MHz, CDCl3) δ 168.4, 136.3, 136.2,
135.88, 135.86, 135.7, 134.3, 134.2, 131.0, 129.3, 129.2, 129.1,
128.2, 127.3, 127.1, 69.4, 69.3, 63.9, 52.7, 45.9, 44.8, 37.9, 31.8,
31.7, 29.1, 27.2, 27.0, 26.8, 25.8, 22.9, 19.2, 18.3, -5.2; HRMS
calcd for C43H65F6O7PSi2Na 917.3809 [M þ Na]þ, found (ESI)
m/z 917.3806, error 0.3 ppm.
(1Z,3E,9Z)-Methyl 11-[(1,1-Dimethylethyl)diphenylsilyloxy]-
9-methylcyclotetradeca-1,3,9-trienecarboxylate, 11. To a stirred
solution of the above allylic alcohol (250 mg, 0.32 mmol) in
dichloromethane (10 mL) was added NaHCO3 (52.0 mg,
0.64 mmol, 2.0 equiv) followed by DMP (240.0 mg, 0.57 mmol,
1.8 equiv). The solution was stirred at 21 °C for 2 h until TLC
indicate no starting material remaining. The reaction mixture
was treated with 20 mL of ether and 10 mL of a solution of satd
NaHCO3 (5 mL) and satd Na2S2O3 (5 mL). The organic phase
was washed again with satd Na2S2O3 (3 ꢀ 5 mL) and dried over
MgSO4. Removal of the solvent afforded 250 mg of the crude
aldehyde 10. This aldehyde was dissolved in 100 mL of dry
toluene followed by addition of 1.0 g of freshly crystallized
18-Crown-6. The resulting solution was transferred to a 250 mL
flask that contained 270 mg of flame-dried K2CO3. The solution
was allowed to stir at 21°C overnight and then 20 mL of water was
added. The organic phase was washed with brine and dried over
MgSO4. Removal of the solvent and purification by flash column
chromatography on silica gel with hexane:ethyl acetate (6:1)
provided 150 mg (90% over 2 steps) of the cyclic triene ester 11
as a 3.5:1 mixture of Z/E isomers. 1H NMR (500 MHz, CDCl3) δ
7.73 (dd, J = 8.0, 1.5 Hz, 2H), 7.65 (dd, J = 8.0, 1.5 Hz, 2H), 7.38
(m,6H), 6.92(dd, J= 15.0, 8.0 Hz, 1H), 5.89 (d, J= 11.0 Hz, 1H),
5.47 (dt, J = 13.0, 5.0 Hz, 1H), 4.95 (d, J = 9.5 Hz, 1H), 4.20 (dt,
J = 10.0, 2.0 Hz, 1H), 3.69 (s, 3H), 2.46 (m, 1H), 2.29 (m, 1H),
1.93 (m, 3H), 1.80-1.40 (m, 6H), 1.37 (s, 3H), 1.02 (s, 9H), 0.85
(m, 2H), 0.57 (m, 1H); 13C NMR (125 MHz, CDCl3) δ 167.6,
143.8, 143.2, 136.2, 134.7, 134.62, 134.58, 129.9, 129.4, 129.0,
128.2, 127.5, 126.7, 125.4, 67.1, 51.0, 35.1, 33.8, 33.61, 32.57, 27.1,
27.0, 24.6, 22.5, 21.4, 19.4 (one low-field carbon not resolved).
(1Z,3E,9Z)-Methyl 11-Hydroxy-9-methylcyclotetradeca-1,3,9-
trienecarboxylate. To a stirred solution of the silyl ether 11
(150 mg, 0.29 mmol) in 1.5 mL of THF was added TBAF
(1.5 mL, 1 M in THF, 5.0 equiv). The resulting solution was
heated at 45-50 °C for 48 h. The solution was allowed to cool to
21 °C and 10 mL of ether was added followed by addition of 2.0 mL
of a pH 7 buffer solution. The aqueous phase was extracted twice
with 5.0 mL of ether. After drying over MgSO4, the ether was
removed in vacuo. The residue was purified by flash column
chromatography on silica gel with hexane:ethyl acetate (3:1) to give
1
52.4 mg (65%) of the secondary alcohol. H NMR (500 MHz,
CDCl3) δ6.92 (dd, J = 15.0, 8.0 Hz, 1H), 6.55 (d, J = 11.0 Hz, 1H),
5.89 (dt, J = 13.0, 5.0 Hz, 1H), 4.95 (d, J = 8.5 Hz, 1H), 4.30 (dt,
J = 10.0, 2.0 Hz, 1H), 3.76 (s, 3H), 2.62 (m, 1H), 2.30 (m, 3H), 1.95
(m, 3H), 1.70 (s, 3H), 1.70-1.40 (m, 5H), 1.24 (m, 2H); 13C NMR
(125 MHz, CDCl3) δ 168.30, 143.2, 141.8, 137.1, 129.2, 128.6, 127.1,
65.8, 51.3, 35.2, 33.3, 27.0, 25.7, 23.1, 21.0 (two high-field carbons
not resolved); HRMS calcd for C17H26O3Na 301.1780 [M þ Na]þ,
found (ESI) m/z301.1772, error 0.8 ppm. During this reaction, some
of the acid (due to hydrolysis) was isolated as well.
(1Z,3E,9Z)-11-Hydroxy-9-methylcyclotetradeca-1,3,9-triene-
carboxylic Acid. To a stirred solution of the above hydroxy ester
(100 mg, 0.36 mmol) in 9.0 mL of methanol was added 3.0 mL of
1 M LiOH slowly. The solution was allowed to stir at 21 °C for
10 d until TLC indicated only a trace of starting material
remaining. The reaction mixture was extracted with 20.0 mL
of ethyl acetate and the organic phase was washed and dried
over MgSO4. The residue was purified by flash column chro-
matography on silica gel with ethyl acetate as eluent to afford
66.4 mg (70%) of the hydroxy acid. 1H NMR (500 MHz,
CDCl3) δ 7.17 (dd, J = 15.0, 11.5 Hz, 1H), 6.64 (d, J = 11.0
Hz, 1H), 5.94 (br d, J = 7.5 Hz, 1H), 4.97 (d, J = 8.5 Hz, 1H),
4.35 (dt, J = 10.0, 2.5 Hz, 1H), 2.61 (m, 1H), 2.30 (m, 3H), 2.00
(m, 2H), 1.63 (d, J = 1.0 Hz, 3H), 1.70-1.10 (m, 8H); 13C NMR
(125 MHz, CD3OD) δ 169.2, 142.8, 142.2, 135.7, 129.4, 128.7,
126.8, 65.0, 34.4, 33.1, 33.0, 26.8, 25.1, 21.9, 20.8 (one high-field
carbon not resolved); HRMS calcd for C16H24O3Na 287.1623
[M þ Na]þ, found (ESI) m/z 287.1625, error 0.2 ppm.
(7Z,13E)-Methyl 2-[Bis(2,2,2-trifluoroethoxy)phosphoryl]-15-
hydroxy-8-methyl-6-[(1,1-dimethylethyl)diphenylsilyloxy]pentadeca-
7,13-dienoate. The above phosphono ester (350 mg, 0.39 mmol) was
dissolved in 9 mL of acetic acid, 6 mL of THF, and 3 mL of water.
The resulting solution was stirred for 5 h and then was extracted
with 30 mL of ether. The organic phase was washed with water and
brine and dried over MgSO4. Removal of the solvent followed by
flash column chromatography on silica gel (hexane:ethyl acetate
1:1) gave 292 mg (96%) of the allylic alcohol. 1H NMR (500 MHz,
CDCl3) δ 7.64 (m, 4H), 7.37 (m, 6H), 5.62 (m, 2H), 5.12 (t, J = 7.5
Hz, 1H), 4.38 (m, 5H), 4.07 (d, J= 4.5 Hz, 2H), 3.72 (d, J=7.0Hz,
3H), 3.04 (m, 1H), 2.00 (m, 2H), 1.90-1.30 (m, 12H), 1.10 (d, J =
1.0 Hz, 3H), 1.02 (s, 9H); 13C NMR (125 MHz, CDCl3) δ 168.4,
135.9, 135.81, 135.79, 135.73, 134.4, 134.3, 132.9, 129.4, 129.2,
129.0, 128.9, 127.73, 127.69, 127.4, 127.1, 69.79, 69.76, 63.6, 52.7,
45.9, 44.8, 39.0, 37.6, 31.94, 31.92, 28.6, 26.9, 26.8, 19.1, 16.0.
J. Org. Chem. Vol. 75, No. 20, 2010 6939

Jung et al.
JOCArticle
(2Z,8E,10E)-3-Methyl-15-oxabicyclo[9.3.2]hexadeca-2,8,10-
trien-16-one, 12. To a solution of the above hydroxy acid (8.0 mg,
0.03 mmol) in THF (300 μL) at 0 °C were added triethylamine
(8.4 μL, 0.06 mmol) and 2,4,6-trichlorobenzoyl chloride (9.0 μL,
0.054 mmol), and the resulting mixture was stirred at 21 °C for 2 h
before dilution with toluene (3.5 mL). This solution was added
dropwise over a period of 10 min to a solution of DMAP (7.5 mg,
0.06 mmol) in toluene (10 mL). This solution was stirred at 21 °C
for 3 h and then 5.0 mL of water was added. The organic phase was
washed with 1 M HCl, satd NaHCO3, and brine and dried over
MgSO4. It was then filtered and concentrated under reduced
pressure. Purification by flash column chromatography on silica
gel afforded 4.5 mg (61%) of the bicyclic lactone 12. 1H NMR (500
MHz, CDCl3) δ 6.21 (m, 2H), 5.63 (dt, J = 13.0, 5.0 Hz, 1H), 5.21
(d, J = 9.5 Hz, 1H), 5.05 (dt, J = 9.0, 3.0 Hz, 1H), 2.30 (m, 3H),
2.01 (m, 1H), 1.91 (m, 3H), 1.80-1.60 (m, 4H), 1.72 (s, 3H), 1.19
(m, 2H), 0.87 (m, 1H); 13C NMR (125 MHz, CDCl3) δ 173.6,
145.1, 135.0, 133.2, 128.6, 127.6, 122.5, 75.9, 33.0, 32.7, 32.5, 29.5,
26.9, 25.7, 25.2, 23.9.
(2Z,8E,10Z)-11-(Hydroxymethyl)-3-methylcyclotetradeca-2,8,
10-trienol, 14. To a stirred solution of the ester silyl ether 11 (100
mg, 0.193 mmol) containing a 3.5:1 mixture of Z/E isomers in 3.0
mL of THF was added DIBAL-H (3.0 mL, 1.0 M in hexane, 15.0
equiv) at -78 °C. The solution was allowed to warm to -50 °C
and was then quenched with satd K-Na tartrate (4.0 mL)
followed by the addition of 20 mL of ether. The solution was
stirred until it became clear. The organic phase was washed with
water and brine and dried over MgSO4. It was then filtered and
concentrated under reduced pressure. The residue, which con-
tained aninseparable mixtureofZ/E isomers, was dissolvedin3.0
mL of THF and TBAF (4.0 mL, 1.0 M in THF, 20.0 equiv) was
added. After being stirred at 21 °C overnight, the reaction was
quenched with a pH 7 buffer solution and was extracted with
30 mL of ether. After removal of the solvent, the residue was
purified by flash column chromatography on silica gel with
hexane:ethyl acetate (2:1) to afford 30.0 mg (78% over 2 steps)
of the diol 14, which was only the Z isomer. 1H NMR (500 MHz,
CDCl3) δ 6.44 (dd, J = 15.0, 11.5 Hz, 1H), 6.10 (d, J = 11.5 Hz,
1H), 5.58 (dt, J = 12.5, 4.5 Hz, 1H), 4.95 (d, J = 9.0 Hz, 1H), 4.56
(m, 2H), 4.10 (d, J = 12.5 Hz, 1H), 2.48 (m, 1H), 2.38 (m, 1H),
2.3-1.9 (m, 4H), 1.65 (s, 3H), 1.8-1.5 (m, 4H), 1.5-1.1 (m, 4H);
13C NMR (125 MHz, CDCl3) δ 138.6, 137.7, 135.7, 131.0,
128.6, 126.9, 65.6, 62.0, 36.1, 33.6, 33.2, 27.0, 26.2, 23.4, 21.4
(one high-field carbon not resolved); HRMS calcd for
C16H26O2Na 273.1830 [M þ Na]þ, found (ESI) m/z 273.1828,
error 0.2 ppm.
15 (10 mg, 0.032 mmol) in 5.0 mL of toluene was heated in a
microwave to 125 °C overnight. The residue was purified by
preparative TLC to afford 7.5 mg (75%) of the silyl enol ether
16. 1H NMR (400 MHz, CDCl3) δ 6.16 (s, 1H), 5.64 (d, J = 11.0
Hz, 1H), 5.52 (dt, J = 11.0, 3.5 Hz, 1H), 5.11 (d, J = 7.5 Hz, 1H),
5.00 (t, J = 7.5 Hz, 1H), 2.90 (m, 1H), 2.71 (m, 1H), 2.15 (m, 1H),
2.12 (m, 1H), 1.85 (m, 4H), 1.66 (d, J = 1.0 Hz, 3H), 1.60-1.10 (m,
7H), 0.87 (m, 1H), 0.25 (s, 3H), 0.23 (s, 3H); 13C NMR (125 MHz,
CDCl3) δ 137.7, 134.6, 131.5, 130.1, 128.4, 124.3, 69.7, 40.3, 30.0,
29.0, 26.6, 26.2, 25.6, 24.6, 23.5, 22.8, -2.4, -3.2.
(2Z,8E,10Z)-3-Methyl-15-oxabicyclo[9.3.2]hexadeca-2,8,
10-triene, 18. To a stirred solution of the diol 14 (15.0 mg, 0.06
mmol) and triethylamine (50 μL, 0.36 mmol, 6.0 equiv) in
dichloromethane (15.0 mL) was added a solution of triphosgene
(27.0 mg, 0.09 mmol, 1.5 equiv) in 2.0 mL of dichloromethane.
The solution was allowed to stir for 20 min at 0 °C and then satd
NaHCO3 was added. The solution was diluted with 20 mL of ether.
After the phases were separated, the organic phase was washed with
water and brine and dried over MgSO4. Removal of the solvent and
purification of residue by flash column chromatography on silica
gel afforded 10.8 mg (65%) of the cyclic ether18, which had already
cyclized to the tetracyclic ether 19 in about 20% yield by NMR. 1H
NMR (500 MHz, benzene-d6) of 18: δ 6.46 (dd, J = 15.0, 11.0 Hz,
1H), 6.15 (d, J = 10.0 Hz, 1H), 5.35 (d, J = 15.0 Hz, 1H), 5.32 (dt,
J = 11.0, 4.5 Hz, 1H), 4.74 (d, J = 10.5 Hz, 1H), 4.25 (t, J = 10.5
Hz, 1H), 3.66 (d, J = 11.5 Hz, 1H), 2.2 (m, 2H), 2.0 (m, 1H), 1.85
(m, 3H), 1.70-0.9 (m, 11H); 13C NMR (125 MHz, benzene-d6) δ
150.3, 139.8, 135.4, 130.8, 130.0, 129.8, 128.1, 69.8, 66.4, 36.9, 33.8,
32.82, 32.79, 28.6, 26.2, 25.5, 23.6.
(()-(4R,4aS,4bS,8aR,10aR)-4,10a-(Epoxymethylene)-4b-methyl-
1,2,3,4,4a,4b,5,6,7,8, 8a,10a-dodecahydrophenanthrene, 19. A de-
gassed solution of the cyclic ether 18 (10.0 mg, 0.036 mmol) in
5.0 mL of toluene was heated to reflux in a sealed tube for 4 h.
Removal of the solvent under reduced pressure afforded 10.0
mg (100%) of the pure tetracyclic ether 19. 1H NMR (500
MHz, benzene-d6) δ 5.55 (dd, J = 9.0, 2.5 Hz, 1H), 5.50 (dd,
J = 9.0, 2.5 Hz, 1H), 4.48 (d, J = 3.5 Hz, 1H), 3.83 (d, J = 8.0
Hz, 1H), 3.67 (d, J = 8.0 Hz, 1H), 2.0 (m, 1H), 1.84 (m, 2H),
1.70-0.70 (m, 16H); 13C NMR (125 MHz, benzene-d6) δ 150.3,
134.8, 131.6, 78.0, 77.0, 64.1, 47.0, 41.7, 38.6, 36.9, 34.7, 34.4,
27.2, 26.1, 22.9, 21.7, 18.9; HRMS calcd for C16H24ONH4
250.2171 [M þ NH4]þ, found (ESI) m/z 250.2165, error
0.6 ppm.
(()-(4R,4aS,4bS,8aR,10aR)-4,10a-(Epoxymethylene)-4b-methy-
lperhydrophenanthrene, 20. To a stirred solution of cyclic ether 19 (3.4
mg, 15 μmol) in 0.6 mL of ethyl acetate was added 10% Pd/C (1.6 mg,
1.5 μmol). The heterogeneous mixture was stirred at 21 °C under a
balloon of hydrogen for 1 h and then filtered through a plug of
silica gel. The eluent was concentrated in vacuo. Further purifi-
cation by flash chromatography on silica gel (hexanes:ethyl acetate
20:1) afforded 2.7 mg (12 μmol, 80%) of the saturated ether 20. 1H
NMR (500 MHz, benzene-d6) δ 4.46 (d, 1H, J = 4 Hz), 3.77 (d, 1H,
J = 8.5 Hz), 3.61 (d, 1H, J = 8 Hz), 1.84 (m, 2H), 1.60 (m, 3H),
1.50-0.70 (m, 14H); 13C NMR (125 MHz, benzene-d6) δ 77.4,
75.1, 61.4, 44.3, 41.3, 36.2, 35.7, 34.6, 33.2, 30.0, 29.6, 26.4, 24.9,
24.4, 22.2, 21.9.
(1Z,3E,9Z)-9,13,13-Trimethyl-12,14-dioxa-13-silabicyclo[9.4.3]-
octadeca-1,3,9-triene, 15. Toastirredsolutionofthe diol14 (18mg,
0.072 mmol) in dichloromethane (6.0 mL) was added triethyla-
mine (25.0 μL, 0.18 mmol, 2.5 equiv) and DMAP (2.0 mg, 0.014
mmol, 0.2 equiv). To this cooled solution (0 °C) was added slowly
a solution of dichloromethane (2.0 mL) containing dichlorodi-
methylsilane (10.0 mg, 0.079 mmol, 1.1 equiv). The solution was
allowed to stir for 20 min and then was quenched with satd
NaHCO3 and extracted with ether (10.0 mL). The organic phase
was washed with water and brine and dried over MgSO4. It was
then filtered and concentrated under reduced pressure. The residue
was purified by flash column chromatography on silica gel with
hexane:ethyl acetate (6:1) and afforded 15.0 mg (68%) of the silyl
ether 15. 1H NMR (400 MHz, CDCl3) δ 6.54 (dd, J = 12.0, 10.0
Hz, 1H), 5.95 (d, J = 11.0 Hz, 1H), 5.58 (dt, J = 12.0, 3.6 Hz, 1H),
4.94 (d, J = 10.0 Hz, 1H), 4.76 (d, J = 12.0 Hz, 1H), 4.73 (m, 1H),
4.12 (d, J = 12.0 Hz, 1H), 2.44 (m, 3H), 2.08 (m, 3H), 1.80 (m,
1H), 1.62 (d, J = 1.0 Hz, 3H), 1.60-1.00 (m, 7H); 13C NMR (125
MHz, CDCl3) δ 137.4, 136.2, 135.2, 130.4, 129.4, 127.0, 67.2, 62.6,
38.8, 35.5, 33.8, 33.7, 27.2, 26.3, 23.2, 21.7, -2.7, -3.4.
Acknowledgment. The authors thank Dr. Saeed Khan for
obtaining and analyzing the X-ray crystallographic data and
Dr. John Greaves, Director, Mass Spectrometry Facility,
University of California, Irvine, for the HRMS data.
Supporting Information Available: Proton and carbon NMR
data for all compounds, X-ray crystallographic data (ORTEP and
CIF file) for compound 19, and Cartesian coordinates and energies
for calculated structures. This material is available free of charge via
the Internet at http://pubs.acs.org.
(1Z,2E,9Z)-9,13,13-Trimethyl-12,14-dioxa-13-silabicyclo[9.4.3]-
octadeca-1(15),2,9-triene, 16. A degassed solution of the silyl ether
6940 J. Org. Chem. Vol. 75, No. 20, 2010