ACS Combinatorial Science
RESEARCH ARTICLE
advantages, such as operational simplicity, good yields in short
reaction times, and easy workup procedures.
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’ EXPERIMENTAL PROCEDURES
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NY, 1996.
General Procedure for Preparation of Spiroindeno[1,2-b]-
pyrido[2,3-d]pyrimidine-5,30-indolines 4. A mixture of 1,3-in-
dandione (1 mmol), amino uracil (1 mmol), and isatin (1 mmol)
in refluxing ethanol (5 mL) was stirred for 3 h. After completion of
the reaction, progress of reaction was monitored using TLC (eluent
EtOAc/n-hexane, 1:3), the reaction mixture was cooled to room
temperature. Then, the precipitated product was filtered and washed
with water (10 mL) and ethanol (5 mL) to afford the pure product 4.
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’ ASSOCIATED CONTENT
S
Supporting Information. Experimental procedures and
b
IR, 1
H NMR, and 13C NMR spectra for compounds 4 and 6. This
acs.org.
’ AUTHOR INFORMATION
Corresponding Author
*E-mail: a_bazgir@sbu.ac.ir. Phone: þ98 21 22401765. Fax:
þ98 21 22403041.
’ ACKNOWLEDGMENT
We gratefully acknowledge financial support from the Re-
search Council of Shahid Beheshti University.
’ REFERENCES
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(15) X-ray data for 4{1,5}:(C21H11Br1N4O4)(DMSO, H2O), M =
559.33 g/mol, triclinic system, space group P1, a = 9.9246(17) Å, b =
11.6238(19) Å, c = 11.6546(18) Å, β = 104.096(13)o, V = 1154.4(3) Å3,
Z = 2, Dc = 1.609 g cm-3, μ(Mo KR)= 1.920 mm-1, crystal dimention of
0.28 ꢀ 0.20 ꢀ 0.08 mm. The structure was solved by using SHELXS. The
structure refinement and data reduction was carried out with SHELXL of
the X-Step32 suite of programs.17 The non-hydrogen atoms were refined
anisotropically by full matrix least-squares on F2 values to final R1 = 0.0840,
R2 = 0.2454, and S = 1.098 with 330 parameters using 6211 independent
reflection (θ range = 1.98-29.27°). Hydrogen atoms were located from
expected geometry and were not refined. Crystallographic data for 4{1,5}
have been deposited with the Cambridge Crystallographic Data Centre.
Copies of the data can be obtained, free of charge, on application to The
Director, CCDC 789416, Union Road, Cambridge CB2 1EZ, U.K. Fax:
þ44 1223 336033. E-mail: deposit@ccdc.cam.ac.uk.
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for Bacteria, which Grows Aerobically, 5th ed.; Approved Standard
M7-A5; NCCLS: Villanova, PA, 2000.
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Stoe & Cie: Darmstadt, Germany, 2000.
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dx.doi.org/10.1021/co1000053 |ACS Comb. Sci. 2011, 13, 96–99