7834
M. Z. Hernandes et al. / Bioorg. Med. Chem. 18 (2010) 7826–7835
ified Eagle’s Medium (DMEM, Sigma Chemical Co., USA) supple-
mented with 10% of fetal calf serum (FCS; Cultilab, Brazil) and
for 3g compound, HRMS and elemental analysis data of all new
compounds) associated with this article can be found, in the online
50
was evaluated in four concentrations (1, 5, 10 and 100
in triplicate. Cultures were incubated in the presence of 3H-thymi-
dine (1
Ci wellꢀ1) for 24 h at 37 °C and 5% CO2. After this period,
l
g mLꢀ1 of gentamycin (Novafarma, Brazil). Each compound
l
g mLꢀ1),
l
the content of the plate was harvested to determine 3H-thymidine
incorporation using a beta-radiation counter (b-matrix 9600, Pack-
ard). The cytotoxicity of the compounds was determined by com-
paring the percentage of 3H-thymidine incorporation (as an
indicator of cell viability) of drug-treated wells in relation to un-
treated wells. Non-cytotoxic concentrations were defined as those
causing a reduction of 3H-thymidine incorporation below 10% in
relation to untreated cells (control).
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The selected compounds were re-suspended in 100 lL [30%
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Acknowledgements
18. (a) Romeiro, N. C.; Aguirre, G.; Hernández, P.; González, M.; Cerecetto, H.;
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This research received support from Pernambuco State Founda-
tion for Science and Technology (FACEPE, Grant #APQ-0123-4.03/
08) and Brazilian National Council of Research (CNPq, Grant
#472880/2009-8). M.V.O.C. holds
a CAPES scholarship, while
J.H.M. acknowledges the Sandler Family Foundation. We also thank
the Federal University of Pernambuco Department of Fundamental
Chemistry for recording the spectra and elemental analyses and
HEMOPE Hospital for supplying mice for the toxicological test.
19. Bryant, C.; Kerr, I. D.; Debnath, M.; Ang, K. K. H.; Ratnam, J.; Ferreira, R. S.;
Jaishankar, P.; Zhao, D.-M.; Arkin, M. R.; McKerrow, J. H.; Brinen, L. S.; Renslo, A.
R. Bioorg. Med. Chem. Lett. 2009, 19, 6218–6221.
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Supplementary data
21. Leite, A. C. L.; Lima, R. S.; Moreira, D. R. M.; Cardoso, M. V. O.; Brito, A. C. G.;
Santos, L. M. F.; Hernandes, M. Z.; Kiperstok, A. C.; Lima, R. S.; Soares, M. B. P.
Bioorg. Med. Chem. 2006, 14, 3749–3757.
CCDC 793133 (3g) contains the supplementary crystallographic
data for this paper. These data can be obtained charge free from