Increased transglycosylation activity of Rhodotorula glutinis endo-β-glucanase in media containing organic solvent

Article abstract of DOI:10.1271/bbb.65.1889

The transglycosylation of p-nitrophenyl-β-D-cellotrioside to cellotetraose catalyzed by endo-1,4-β-glucanase (cellulase, EC 3.2.1.4) from a psychrotrophic yeast, Rhodotorula glutinis KUJ 2731, was increased by addition of a miscible organic solvent in the reaction mixture. Among various organic solvents tested, acetone was most effective. The transglycosylation activity increased with an increase in acetone concentrations, while hydrolysis activity was suppressed. The transglycosylation preferably occurred at acidic pH with the optimum pH at 2 in 10 mM Gly-HCl buffer. The optimum temperature of transglycosylation was found to be 50°C in the presence of 40% acetone.

Full text of DOI:10.1271/bbb.65.1889

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Increased Transglycosylation Activity of Rhodotorula
glutinis Endo-β-Glucanase in Media Containing
Organic Solvent
Tadao OIKAWAab, Yasuyuki TSUKAGAWAa, Masashi CHINOa & Kenji SODAab
a Department of Biotechnology, Faculty of Engineering, Kansai University
b Kansai University High Technology Research Center
Published online: 22 May 2014.
To cite this article: Tadao OIKAWA, Yasuyuki TSUKAGAWA, Masashi CHINO & Kenji SODA (2001) Increased
Transglycosylation Activity of Rhodotorula glutinis Endo-β-Glucanase in Media Containing Organic Solvent, Bioscience,
Biotechnology, and Biochemistry, 65:8, 1889-1892, DOI: 10.1271/bbb.65.1889
To link to this article: http://dx.doi.org/10.1271/bbb.65.1889
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