Discovery of a potent thiadiazole class of histamine H3 receptor antagonist for the treatment of diabetes

Article abstract of DOI:10.1021/ml200250t

A series of novel 2-piperidinopiperidine thiadiazoles were synthesized and evaluated as new leads of histamine H₃ receptor antagonists. The 4-(5-([1,4-bipiperidin]-1-yl)-1,3,4-thiadiazol-2-yl)-2-(pyridin-2-yl)morpholine (5u) displayed excellent potency and ex vivo receptor occupancy. Compound 5u was also evaluated in vivo for antidiabetic efficacy in STZ diet-induced obesity type 2 diabetic mice for 2 or 12 days. Non-fasting glucose levels were significantly reduced as compared with vehicle-treated mice. In addition, 5u dose dependently blocked the increase of HbA_1c after 12 days of treatment.

Full text of DOI:10.1021/ml200250t

Discovery of a potent thiadiazole class of histamine H3 receptor
antagonist for the treatment of diabetes
Ashwin U. Rao,*,§ Ning Shao,§ Robert G. Aslanian,§ Tin-Yau Chan,§ Sylvia J. Degrado,§
Li Wang,§ Brian McKittrick,§ Mary Senior,§ Robert E. West Jr.,† Shirley M. Williams,†
Ren-Long Wu,† Joyce Hwa,† Bhuneshwari Patel,† Shuqin Zheng,† Christopher Sondey,†
Anandan Palani§
§Department of Medicinal Chemistry, Merck Research Laboratories, 2015 Galloping Hill Road,
Kenilworth, NJ 07033, USA
† Cardiovascular/Metabolic Disease, Merck Research Laboratories, 2015 Galloping Hill Road, Kenilworth,
NJ 07033, USA
Experimental Procedures
Analytical methods: All reactions were carried out under nitrogen atmosphere in
anhydrous conditions, unless otherwise noted. Solvents were purchased from Aldrich and
Acros without further purification. Reagents and chemicals were purchased from
commercial sources with purity ≥ 95% without further purification. Flash
chromatography was carried out with EM Science silica gel 60 (neutral, 230-400 mesh).
1H NMR and 13C NMR were recorded on a Bruker Avance 500 NMR Spectrometer.
LCMS analyses were performed on Applied Biosystem API-150. HRMS analyses were
recorded on a Micromass QT of Ultima API US Mass Spectrometer by FAB.
All of the biologic assays and experiments with animal models performed in Merck
& Co., Inc. were in accordance with all national or local guidelines and regulations.
Determination of binding affinities in a [3H]-N-α-methylhistamine human and
mouse recombinant assay: For binding assays, membranes (P2 pellet) from rHu H3-
HEK cells (3 μg protein) were incubated in 200 μL 50 mM Tris·HCl, pH 7.4, with 1 nM
[3H]-N-α-methylhistamine (82 Ci/mmol) and compounds at concentrations equivalent to
half orders of magnitude over a five-order of magnitude range. Nonspecific binding was
determined in the presence of 10-5 M thioperamide. After 30 min at 30 °C, assay
mixtures were filtered through 0.3% polyethylenimine-soaked GF/B glass fiber filters,
which were rinsed thrice with buffer, dried, impregnated with Meltilex wax scintillant,
and counted. Ki values were determined from curves fit to the data using GraphPad
Prism nonlinear, least-squares, curve-fitting program.
Determination of ex vivo receptor occupancy: ICR, Imprinting Control Region mice
(from Taconics, nomenclature IcrTacICR). Individual mouse cortexes were dissected and
homogenized in ice cold assay buffer, 50 mM Na2HPO4–KH2PO4 buffer (pH 6.8).
Samples were then frozen at –80 °C for at least 12 hours. Protein concentration was
determined by BCA Protein Assay. A 30 min room temperature incubation was
performed, containing 140 μg/assay homogenized cortex sample, 0.1% BSA, 1 nM [3H]-
N-α-Methylhistamine and 10 μM thioperamide for non-specific binding or assay buffer
for total binding. Incubations were performed in quadruplet and stopped by rapid

filtration on a Brandel Harvester using Unifilter-96, GF/B plates presoaked in 0.3% PEI
for 30 min. The remaining radioactivity was measured on a Packard TopCount-NTX.
Specific binding was calculated by subtracting the non-specific binding from the total.
Determination of effects on non-fasting glucose in mouse: Five week old male ICR
mice (Taconic, Tac:Icr:Ha(ICR)fBR) were fed high fat/cholesterol diet (45% Kcal fat and
0.12% cholesterol, Research Diets D04012801) for 3 weeks, and were given
streptozotocin (STZ) intraperitoneally at 80 mg/kg to induce type 2 diabetes. T2D mice
were chosen for the study two weeks after STZ injection (n=12 per group, with non-
fasting glucose between 250 and 500 mg/dl) and were balanced into groups. Compounds
were administered by PO dosing with 0.4% MC daily before onset of dark. Non-fasting
glucose was monitored daily at 4 hours after the lights were on. Individual body weight
and food intake were monitored daily.
Determination of 12 day change in HbA1c assay: HbA1c was monitored before and
after the treatment by A1CNow kit from Bayer Health Care.
General procedure for the preparation of analogs was illustrated by the
synthesis of compound 5c
To a heterogenous mixture of 2-amino-1,3,4-thiadiazole (1) (100 g, 988.8 mmol)
and sodium acetate (324.5 g, 3955.2 mmol, 4.0 eq.) in acetic acid (1.4 L) was added
bromine (2 M in AcOH, 524 mL, 1048 mmol, 1.06 eq.) dropwise over 2 h under cooling
with ice. The reaction mixture was allowed to stir at room temperature overnight. The
reaction mixture gradually solidified upon stirring overnight. Added 500 mL of AcOH
and the reaction was stirred longer upon which the excess AcOH was concentrated and
H2O (1 L) was added. The reaction mixture was agitated and the solids were filtered,
washed with water and dried over P2O5 under vacuum to yield 2 (162.4 g, 91%) as a
brown solid.
To a heterogeneous solution of CuBr2 (37.25 g, 166.6 mmol, 1.2 eq.) and t-
BuNO2 (24.8 mL, 208.31 mmol, 1.5 eq.) in CH3CN (600 mL) was added 2-amino-5-
bromo-1,3,4-thiadiazole 2 (25.0 g, 138.87 mmol) slowly and stirred at room temperature
under nitrogen overnight. The reaction mixture was quenched with 700 mL of saturated
NH4Cl (aq.) and extracted with diethyl ether (3 x 500 mL) and the organic layer was
dried over MgSO4, filtered and concentrated in vacuo to provide the crude product which

was triturated in 200 mL MeOH. The solid product was collected by filtration to provide
23.7 g of compound 3 (Yield: 70%).
To a solution of 2,5-dibromo-1,3,4-thiadiazole 3 (10 g, 41 mmol) and 4-
piperidinopiperidine (8.3 g, 49.2 mmol, 1.2 eq.) in 1,4-dioxane (150 mL) was added N,N-
diisopropylethylamine (8.6 mL, 49.2 mmol, 1.2 eq.). The resulting reaction was heated to
120 oC under nitrogen and allowed to stir at this temperature for 2 h, then cooled to room
temperature. The cooled solution was filtered and the collected solid was dried over
vacuum to provide 8.6 g of compound 4 (Yield: 63%).
To a solution of compound 4 (100 mg, 0.30 mmol) in a mixture of PhCF3 (2.0
mL) and 1,4-dioxane (1.0 mL) was added 2-(pyridin-2-yl)morpholine (59 mg, 0.36 mmol,
1.2 eq.) and N,N-diisopropylethylamine (132 μL, 0.75 mmol, 2.5 eq.). The resulting
o
reaction mixture was heated in a microwave at 220 C for 3 h, then cooled to room
temperature and concentrated in vacuo. The residue was purified by reverse phase
column chromatography to provide 75 mg of compound 5r (Yield: 60%).
LC/MS conditions: Agilent 6140 Quadrupole LC/MS System; Column Zorbax SB-C18,
3.0 x 50.0 mm, 1.8 micron; Mobile phase A: H2O/0.05% TFA/0.5% acetic acid; Mobile
phase B: acetonitrile/0.5% acetic acid; Flow: 1.0 ml/min; Gradient: 0 min 10% B, 1.5 min
95% B, 2.7 min 95% B, 2.8 min 10% B, Stop 3.6 min; Column temperature: 50 oC.

1-(5-([1,4'-bipiperidin]-1'-yl)-1,3,4-thiadiazol-2-yl)-3-cyclopentylimidazolidin-2-one:
1H NMR (CDCl3, 500 MHz) δ 4.32 (p, 1 H, J = 8 Hz, J = 16 H), 4.04 (dd, 2 H, J = 8 Hz, J
= 9.5 Hz), 3.98-3.95 (m, 2 H), 3.55 (dd, 2 H, J = 7 Hz, J = 8 Hz), 3.02 (dt, 2 H, J = 2.5
Hz, J = 12.5 Hz, J = 14.5 Hz), 2.65-2.48 (m, 5 H), 1.96-1.85 (m, 5 H), 1.74-1.56 (m, 11
H), 1.51-1.42 (m, 2 H) ppm; ES-LC/MS (M + H)+ = 405.2 (98% LC/MS purity).
1-(5-([1,4'-bipiperidin]-1'-yl)-1,3,4-thiadiazol-2-yl)-3-phenyltetrahydropyrimidin-
2(1H)-one: 1H NMR (CDCl3, 500 MHz) δ 7.42-7.39 (m, 1 H), 7.31-7.29 (m, 1 H), 7.27-
7.24 (m, 3 H), 4.23 (t, 2H, J = 6.5 Hz), 4.05-4.00 (m, 2 H), 3.81 (t, 2 H, J = 6 Hz), 3.04 (t,
2 H, J = 14 Hz), 2.90-2.6 (m, 5 H), 2.32-2.28 (m, 2 H), 2.21-2.08 (m, 2 H), 1.86-1.53 (m,
8 H) ppm; ES-LC/MS (M + H)+ = 427.2 (99% LC/MS purity).
N
N
O
N
N
S
N
5c
1
1-(5-([1,4'-bipiperidin]-1'-yl)-1,3,4-thiadiazol-2-yl)piperidin-2-one: H NMR (CDCl3,
500 MHz) δ 4.10 (t, 2 H, J = 6 Hz), 4.02 (dd, 2 H, J = 2.5 Hz, J = 11 Hz), 3.03 (dt, 2 H, J
= 2.5 Hz, J = 12.5 Hz, J = 15 Hz), 2.63 (t, 2 H, J = 7 Hz), 2.50 (t, 4 H, J = 5 Hz), 2.01-
1.96 (m, 2 H), 1.94-1.87 (m, 4 H), 1.70-1.56 (m, 7 H), 1.45-1.42 (m, 2 H) ppm; ES-
LC/MS (M + H)+ = 350.2 (99% LC/MS purity).
N
N
N
N
S
F
F
N
5d
1
2-([1,4'-bipiperidin]-1'-yl)-5-(3,3-difluoropyrrolidin-1-yl)-1,3,4-thiadiazole: H NMR
(CD3OD, 500 MHz) δ 4.03-3.98 (m, 4 H), 3.86 (t, 2 H, J = 7.5 Hz), 3.57-3.53 (m, 3 H),
3.06 (t, 2 H, J = 12 Hz), 2.73-2.65 (m, 2 H), 2.29-2.27 (m, 2 H), 2.02-1.85 (m, 8 H), 1.59-
1.53 (m, 2 H) ppm; ES-LC/MS (M + H)+ = 358.2 (99% LC/MS purity).
N
S
N
N
N
N
OMe
5g

2-([1,4'-bipiperidin]-1'-yl)-5-(3-methoxypiperidin-1-yl)-1,3,4-thiadiazole: 1H NMR
(CD3OD, 500 MHz) δ 3.95 (d, 2 H, J = 13 Hz), 3.71-3.48 (m, 8 H), 3.38 (s, 3 H), 3.32-
3.25 (m, 2 H), 3.04 (t, 2 H, J = 12.5 Hz), 2.25 (d, 2 H, J = 12 Hz), 2.02-1.81 (m, 8 H),
1.7-1.51 (m, 4 H) ppm; ES-LC/MS (M + H)+ = 366.2 (99% LC/MS purity).
1
1-(5-([1,4'-bipiperidin]-1'-yl)-1,3,4-thiadiazol-2-yl)piperidin-3-ol: H NMR (CD3OD,
500 MHz) δ 3.95 (d, 2 H, J = 11 Hz), 3.72 (dd, 1 H, J = 2.5 Hz, J = 13.5 Hz), 3.63-3.44
(m, 4 H), 3.32-3.28 (m, 3 H), 3.07 (t, 2 H, J = 10.5 Hz), 2.27 (d, 2 H, J = 11.5 Hz), 2.06-
1.86 (m, 7 H), 1.77-1.68 (m, 3 H), 1.56-1.39 (m, 2 H) ppm; ES-LC/MS (M + H)+ = 352.2
(94% LC/MS purity).
2-([1,4'-bipiperidin]-1'-yl)-5-(3-fluoropiperidin-1-yl)-1,3,4-thiadiazole: 1H NMR
(CD3OD, 500 MHz) δ 3.96 (dd, 2 H, J = 2 Hz, J = 11.5 Hz), 3.92-3.85 (m, 1 H), 3.77-
3.67 (m, 2 H), 3.56-3.46 (m, 5 H), 3.09-3.04 (m, 3 H), 2.25 (d, 2 H, J = 12 Hz), 2.09-1.76
(m, 12 H), 1.59-1.51 (m, 2 H) ppm; ES-LC/MS (M + H)+ = 354.2 (93% LC/MS purity).
4-(5-([1,4'-bipiperidin]-1'-yl)-1,3,4-thiadiazol-2-yl)-2,6-dimethylmorpholine:
1H
NMR (CD3OD, 500 MHz) δ 3.96 (dd, 2 H, J = 2.0 Hz, J = 11 Hz), 3.82-3.77 (m, 2 H),
3.62 (dd, 2 H, J = 2 Hz, J = 12.5 Hz), 3.56-3.50 (m, 3 H), 3.36-3.30 (m, 2 H), 3.09-2.99
(m, 4 H), 2.27 (d, 2 H, J = 12.5 Hz), 2.02-1.85 (m, 7 H), 1.57-1.53 (m, 1 H), 1.24 (d, 6 H,
J = 6.5 Hz) ppm; ES-LC/MS (M + H)+ = 366.2 (99% LC/MS purity).
2-([1,4'-bipiperidin]-1'-yl)-5-(5',6'-dihydro-[2,3'-bipyridin]-1'(2'H)-yl)-1,3,4-
thiadiazole: 1H NMR (CD3OD, 500 MHz) δ 8.52 (d, J = 5.0 Hz, 1H), 8.05 (ddd, J = 1.5,
7.5, 8.0 Hz, 1H), 7.78 (d, J = 8.0 Hz, 1H), 7.50 (dd, J = 5.5, 7.5 Hz, 1H), 6.82 (m, 1H),
4.41 (dd, J = 2.0, 4.0 Hz, 2H), 3.88 (dt, J = 2.5, 13.5 Hz, 2H), 3.59 (t, J = 6.0 Hz, 2H),
3.44 (d, J = 11.5 Hz, 2H), 3.37 (m, 1H), 3.12 (t, J = 11.0 Hz, 2H), 2.94 (t, J = 12.5 Hz,

2H), 2.53 (m, 2H), 2.11 (d, J = 12.0 Hz, 2H), 1.91-1.65 (m, 7H), 1.44 (m, 1H) ppm; ES-
LC/MS (M + H)+ = 411.2 (96% LC/MS purity).
2-([1,4'-bipiperidin]-1'-yl)-5-(3-(pyrimidin-2-yl)-5,6-dihydropyridin-1(2H)-yl)-1,3,4-
thiadiazole: 1H NMR (CD3OD, 500 MHz) δ 8.65 (d, J = 4.5 Hz, 1H), 7.40 (m, 1H), 7.22
(dd, J = 4.5, 5.0 Hz, 1H), 4.44 (br, 2H), 3.88 (d, J = 11.0 Hz, 2H), 3.62 (t, J = 5.5 Hz,
2H), 3.44 (d, J = 12.0 Hz, 2H), 3.38 (m, 1H), 3.14 (t, J = 14.5 Hz, 2H), 2.95 (t, J = 14.5
Hz, 2H), 2.54 (m, 2H), 2.12 (d, J = 13.0 Hz, 2H), 1.92-1.67 (m, 7H), 1.45 (m, 1H) ppm;
ES-LC/MS (M + H)+ = 412.2 (98% LC/MS purity).
2-([1,4'-bipiperidin]-1'-yl)-5-(5',6'-dihydro-[2,3'-bipyridin]-1'(4'H)-yl)-1,3,4-
thiadiazole: 1H NMR (CD3OD, 500 MHz) δ 8.34 (d, J = 5.5 Hz, 1H), 8.22 (t, J = 9.0 Hz,
1H), 7.98 (d, J = 8.5 Hz, 1H), 7.92 (s, 1H), 7.51 (t, J = 7.0 Hz, 1H), 3.92 (m, 2H), 3.78
(dd, J = 5.5, 6.0 Hz, 2H), 3.43 (d, J = 6.5 Hz, 2H), 3.38 (m, 1H), 3.14 (t, J = 13.5 Hz,
2H), 2.94 (t, J = 11.5 Hz, 2H), 2.54 (t, J = 6.0 Hz, 2H), 2.14-2.08 (m, 4H), 1.90-1.68 (m,
7H), 1.43 (m, 1H) ppm; ES-LC/MS (M + H)+ = 411.2 (96% LC/MS purity).
2-([1,4'-bipiperidin]-1'-yl)-5-(3-methoxy-3-(pyridin-2-yl)piperidin-1-yl)-1,3,4-
thiadiazole: 1H NMR (CDCl3, 500 MHz) δ 8.58 (d, J = 5.0 Hz, 1H), 7.72 (t, J = 8.5 Hz,
1H), 7.56 (d, J = 8.5 Hz, 1H), 7.21 (dd, J = 5.0, 7.5 Hz, 1H), 3.91-3.73 (m, 4H), 3.64 (d, J
= 13.0 Hz, 1H), 3.11 (s, 3H), 2.96 (t, J = 10.5 Hz, 2H), 2.52-2.37 (m, 4H), 2.22-2.00 (m,
3H), 1.85 (m, 2H), 1.69-1.53 (m, 9H), 1.43 (m, 2H) ppm; ES-LC/MS (M + H)+ = 443.2
(95% LC/MS purity).
1
1-(5-([1,4'-bipiperidin]-1'-yl)-1,3,4-thiadiazol-2-yl)-3-(pyridin-2-yl)piperidin-3-ol: H
NMR (CD3OD, 500 MHz) δ 8.55 (d, J = 1.0, 5.0 Hz, 1H), 8.26 (ddd, J = 1.5, 7.5, 8.5 Hz,
1H), 7.95 (d, J = 8.0 Hz, 1H), 7.68 (ddd, J = 1.0, 5.5, 7.5 Hz, 1H), 3.76-3.68 (m, 4H),
3.57 (d, J = 14.0 Hz, 1H), 3.34-3.25 (m, 4H), 3.08 (t, J = 13.5 Hz, 2H), 2.84 (t, J = 12.5

Hz, 2H), 2.17 (ddd, J = 4.0, 13.0, 13.0 Hz, 1H), 2.07-1.99 (m, 3H), 1.86-1.57 (m, 10H),
1.34 (m, 1H) ppm; ES-LC/MS (M + H)+ = 429.2 (99% LC/MS purity).
2-([1,4'-bipiperidin]-1'-yl)-5-(3-fluoro-3-(pyridin-2-yl)piperidin-1-yl)-1,3,4-
thiadiazole: 1H NMR (CD3OD, 500 MHz) δ 8.48 (d, J = 5.0 Hz, 1H), 7.81 (ddd, J = 2.0,
7.5, 7.5 Hz, 1H), 7.56 (dd, J = 1.5, 7.5 Hz, 1H), 7.30 (ddd, J = 1.5, 5.0, 8.0 Hz, 1H), 3.98-
3.79 (m, 5H), 3.41-3.32 (m, 4H), 3.13 (t, J = 12.5 Hz, 2H), 2.91 (t, J = 14.0 Hz, 2H), 2.25
(m, 1H), 2.10-2.00 (m, 3H), 1.88-1.64 (m, 9H), 1.40 (m, 1H) ppm; ES-LC/MS (M + H)+
= 431.2 (95% LC/MS purity).
1-(5-([1,4'-bipiperidin]-1'-yl)-1,3,4-thiadiazol-2-yl)-3-(pyrimidin-2-yl)piperidin-3-ol:
1H NMR (CD3OD, 500 MHz) δ 8.72 (d, J = 5.0 Hz, 2H), 7.31 (dd, J = 5.0, 5.0 Hz, 1H),
4.04 (d, J = 13.5 Hz, 1H), 3.83 (m, 2H), 3.73 (m, 1H), 3.66 (m, 1H), 3.46-3.32 (m, 4H),
3.12 (m, 2H), 2.92 (m, 2H), 2.21 (ddd, J = 1.5, 4.0, 11.5 Hz, 1H), 2.10-2.03 (m, 3H),
1.94-1.63 (m, 9H), 1.42 (m, 1H) ppm; ES-LC/MS (M + H)+ = 430.2 (97% LC/MS
purity).
4-(5-([1,4'-bipiperidin]-1'-yl)-1,3,4-thiadiazol-2-yl)-2-(pyridin-2-yl)morpholine: 1H
NMR (CDCl3, 500 MHz) δ 8.62 (d, 1 H, J = 4.5 Hz), 7.78 (dt, 1 H, J = 1.5 Hz, J = 7.5
Hz, J = 9.0 Hz), 7.54 (d, 1 H, J = 7.5 Hz), 7.28 (dd, 1 H, J = 4.5 Hz, J = 7.0 Hz), 4.81
(dd, 1 H, J = 2.5 Hz, J = 10.5 Hz), 4.20 (dd, 1 H, J = 3.5 Hz, J = 12.0 Hz), 4.03-3.84 (m,
4 H), 3.31 (dt, 1 H, J = 3.5 Hz, J = 12.5 Hz, J = 15.5 Hz), 3.20 (t, 1 H, J = 10.5 Hz), 3.06
(t, 2 H, J = 12.5 Hz), 2.61-2.47 (m, 4 H), 1.91 (d, 2 H, J = 12 Hz), 1.72-1.64 (m, 7 H),
1.50 (d, 2 H, J = 1.5 Hz) ppm; ES-LC/MS (M + H)+ = 415.2 (99% LC/MS purity).
4-(5-([1,4'-bipiperidin]-1'-yl)-1,3,4-thiadiazol-2-yl)-2-(pyrazin-2-yl)morpholine: 1H
NMR (CDCl3, 500 MHz) δ 8.85 (s, 1 H), 8.59 (dd, 2 H, J = 2.5 Hz, J = 7.5 Hz), 4.88 (dd,
1 H, J = 2.5 Hz, J = 10.0 Hz), 4.22 (dd, 1 H, J = 4.0 Hz, J = 12 Hz), 4.02 (dd, 2 H, J = 2.5

Hz, J = 11.5 Hz), 3.91 (d, 2 H, J = 13 Hz), 3.81 (dd, 1 H, J = 1.5 Hz, J = 13 Hz), 3.34 (dt,
1 H, J = 3.5 Hz, J = 12 Hz, J = 15.5 Hz), 3.23 (t, 1 H J = 10.5 Hz), 3.06 (t, 2 H, J = 13
Hz), 2.57-2.55 (m, 3 H), 2.53-2.47 (m, 1 H), 1.92 (d, 2 H, J = 12 Hz), 1.75-1.58 (m, 7 H),
1.50-1.48 (m, 2 H) ppm; ES-LC/MS (M + H)+ = 416.2 (100% LC/MS purity).
4-(5-([1,4'-bipiperidin]-1'-yl)-1,3,4-thiadiazol-2-yl)-2-(pyridin-2-yl)morpholine: 1H
NMR (CDCl3, 500 MHz) δ 8.62 (d, 1 H, J = 4.5 Hz), 7.78 (dt, 1 H, J = 2 Hz, J = 8 Hz, J
= 9.5 Hz), 7.54 (d, 1 H, J = 7.5 Hz), 7.28 (dd, 1 H, J = 5.0 Hz, J = 7.5 Hz), 4.81 (dd, 1 H,
J = 2.5 Hz, J = 10.5 Hz), 4.20 (dd, 1 H, J = 3.0 Hz, J = 11.5 Hz), 4.03-3.85 (m, 4 H), 3.31
(dt, 1 H, J = 3.5 Hz, J = 12.5 Hz, J = 16.0 Hz), 3.20 (t, 1 H, J = 10.5 Hz), 3.05 (t, 2 H, J =
12.5 Hz), 2.60-2.46 (m, 4 H), 1.91 (d, 2 H, J = 12 Hz), 1.74-1.59 (m, 7 H), 1.51-1.47 (m,
2 H) ppm; ES-LC/MS (M + H)+ = 415.2 (98% LC/MS purity).
4-(5-([1,4'-bipiperidin]-1'-yl)-1,3,4-thiadiazol-2-yl)-2-(pyridin-2-yl)morpholine: 1H
NMR (CDCl3, 500 MHz) δ 8.62 (d, 1 H, J = 4.5 Hz), 7.78 (t, 1 H, J = 7.5 Hz), 7.54 (d, 1
H, J = 7.5 Hz), 7.28 (dd, 1 H, J = 5.0 Hz, J = 7.5 Hz), 4.81 (dd, 1 H, J = 2.5 Hz, J = 10.5
Hz), 4.20 (dd, 1 H, J = 3.0 Hz, J = 11.5 Hz), 4.03-3.85 (m, 4 H), 3.31 (dt, 1 H, J = 3.5 Hz,
J = 12.5 Hz, J = 16.0 Hz), 3.20 (t, 1 H, J = 10.5 Hz), 3.05 (t, 2 H, J = 12.5 Hz), 2.61-2.46
(m, 4 H), 1.94-1.91 (m, 2 H), 1.75-1.60 (m, 7 H), 1.51-1.47 (m, 2 H) ppm; 13C NMR
(CDCl3) δ 165.4, 165.0, 158.1, 149.0, 136.7, 122.8, 120.6, 77.5, 66.0, 62.2, 55.5, 50.1,
50.0, 48.4, 27.1, 26.2, 24.6. ES-LC/MS (M + H)+ = 415.2 (98% LC/MS purity).