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solution, in a total volume of 0.5 mL. The reaction was initiated
by the addition of NADPH at 30 °C. After 30 min, the reaction
was stopped by the addition of 150 lL of 0.5 M HCl. Then, 0.5 mL
of 6 M NaOH containing 10 mM imidazole was added, and the
mixture was heated at 60 °C for 20 min to convert NADP into a
fluorescent product. The fluorescence intensity was measured
using a fluorescence microplate reader (FLUOstar OPTIMA, BMG
Labtechnologies) at an excitation wavelength of 360 nm and an
emission wavelength of 460 nm. Each test sample was dissolved
in DMSO and the measurements were performed in duplicate,
and IC50 values were determined graphically. The concentration
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of b-nicotinamide adenine dinucleotide phosphate (NADP). An al-
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3.8. Statistics
Values are expressed as means S.E.M. One-way analysis of
variance (ANOVA) followed by Dunnett’s test was used for statisti-
cal analysis. Probability (p) values less than 0.05 were considered
significant.
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Acknowledgments
28. Yoshikawa, M.; Morikawa, T.; Matsuda, H.; Tanabe, G.; Muraoka, O. Bioorg. Med.
Chem. 2002, 10, 1547.
O.M., T.M., and K.N. were supported by a Grant-in Aid for Scien-
tific Research from ‘High-tech Research Center’ Project for Private
Universities: matching fund subsidy from a Grant-in Aid for Scien-
tific Research from the Ministry of Education, Culture, Sports, Sci-
ence and Technology (MEXT) of Japan, 2007–2011 and also
supported by a Grant-in Aid for Scientific Research by Japan Society
for the Promotion of Science (JSPS). M.Y. and H.M. were supported
by the 21st COE program, Academic Frontier Project, and a Grant-
in Aid for Scientific Research from MEXT.
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