Chemical synthesis of 4-thiouridine-containing substrate analogues of tRNA:pseudouridine-55 synthase: Photocross-linking studies

Article abstract of DOI:10.1080/07328319808004327

For site specific incorporation of 4-thiouridine into oligoribonucleotides a new phosphoramidite is proposed. It makes use of the S-pivaloyloxymethyl group for the protection of the thiol function. This group is easily introduced and removed without modification of the standard protocol for solid phase synthesis of RNA. Three 4-thiouridine-containing oligoribonucleotides (21-mers), corresponding to tRNA minisubstrates of yeast tRNA:pseudouridine-55 synthase (Pus4) were prepared. These 4-thiouridine containing substrates were characterized and used as photoaffinity probe of the enzyme:substrate complex. Irradiation resulted in the specific photocross-linking of these oligoribonucleotides with purified recombinant tRNA:pseudouridine-55 synthase.

Full text of DOI:10.1080/07328319808004327

Nucleosides and Nucleotides
ISSN: 0732-8311 (Print) (Online) Journal homepage: http://www.tandfonline.com/loi/lncn19
Chemical Synthesis of 4-Thiouridine-Containing
Substrate Analogues of tRNA:Pseudouridine-55
Synthase: Photocross-Linking Studies
Hubert F. Becker , Henri Grosjean & Jean-Louis Fourrey
To cite this article: Hubert F. Becker , Henri Grosjean & Jean-Louis Fourrey (1998) Chemical
Synthesis of 4-Thiouridine-Containing Substrate Analogues of tRNA:Pseudouridine-55
Synthase: Photocross-Linking Studies, Nucleosides and Nucleotides, 17:12, 2403-2416, DOI:
10.1080/07328319808004327
To link to this article: http://dx.doi.org/10.1080/07328319808004327
Published online: 16 Aug 2006.
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Date: 11 June 2016, At: 11:23