9800 Nucleic Acids Research, 2012, Vol. 40, No. 19
homology domain, and nuclear localization signal. Mol. Cell
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repair (25,46,47). Finally our results suggest that dosage in
the therapeutic use of 5azaC-dR (dicitabine) (48–50) could
be better controlled by the use of GuaUre-dR.
13. Rogstad,D.K., Herring,J.L., Theruvathu,J.A., Burdzy,A.,
Perry,C.C., Neidigh,J.W. and Sowers,L.C. (2009) Chemical
decomposition of 5-aza-20-deoxycytidine (Decitabine): kinetic
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SUPPLEMENTARY DATA
Supplementary Table 1 and Supplementary Figures 1–4.
ACKNOWLEDGEMENTS
16. Friedman,S. (1982) Bactericidal effect of 5-azacytidine on
Escherichia coli carrying EcoRII restriction-modification enzymes.
J. Bacteriol., 151, 262–268.
17. Pfleiderer,W. (2007) Universal 2-(4-nitrophenyl)ethyl and 2-
(4-nitrophenyl)ethoxycarbonyl protecting groups for nucleosides
and nucleotides. Curr. Protoc. Nucleic Acid Chem., Chapter 2,
Unit 2 13.
We would like to thank Denise Keen Mass Spectrometry
& Proteomics Core of the City of Hope Comprehensive
Cancer Center for mass spectrometry analysis.
FUNDING
18. Guimil Garcia,R., Brank,A.S., Christman,J.K., Marquez,V.E. and
Eritja,R. (2001) Synthesis of oligonucleotide inhibitors of DNA
(Cytosine-C5) methyltransferase containing 5-azacytosine residues
at specific sites. Antisense Nucleic Acid Drug Dev., 11, 369–378.
19. Liu,M.C., Luo,M.Z., Mozdziesz,D.E., Lin,T.S., Dutschman,G.E.,
Cheng,Y.C. and Sartorelli,A.C. (1999) Synthesis of
20-methylene-substituted 5-azapyrimidine, 6-azapyrimidine, and
3-deazaguanine nucleoside analogues as potential antitumor/
antiviral agents. Nucleosides Nucleotides, 18, 55–72.
20. Diaz,A.R., Eritjaa,R. and Garcia,R.G. (1997) Synthesis of
oligodeoxynucleotides containing 2-substituted guanine derivatives
using 2-fluoro-20-deoxyinosine as common nucleoside precursor.
Nucleosides, Nucleotides Nucleic Acids, 16, 2035–2051.
21. Alul,R.H., Singman,C.N., Zhang,G.R. and Letsinger,R.L. (1991)
Oxalyl-CPG: a labile support for synthesis of sensitive
oligonucleotide derivatives. Nucleic Acids Res., 19, 1527–1532.
22. Pon,R.T. and Yu,S. (1997) Hydroquinone-O,O0-diacetic acid
(‘Q-linker’) as a replacement for succinyl and oxalyl linker arms
in solid phase oligonucleotide synthesis. Nucleic Acids Res., 25,
3629–3635.
23. Dignam,J.D., Lebovitz,R.M. and Roeder,R.G. (1983) Accurate
transcription initiation by RNA polymerase II in a soluble extract
from isolated mammalian nuclei. Nucleic Acids Res., 11,
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24. Okuwaki,M. and Verreault,A. (2004) Maintenance DNA
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25. Kho,M.R., Baker,D.J., Laayoun,A. and Smith,S.S. (1998) Stalling
of human DNA (cytosine-5) methyltransferase at single-strand
conformers from a site of dynamic mutation. J. Mol. Biol., 275,
67–79.
US National Cancer Institute of the National Institutes
of Health [CA10252 to S.S.S., CA136055 to S.S.S.
and The City of Hope Cancer Center Support Grant
5P30CA033572-27]. Funding for open access charge:
Ensign Fund.
Conflict of interest statement. None declared.
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