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ChemComm
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After the addition of H2S to the above cells for 10 min, the
fluorescence returned to its original state (Figure 4c) because H2S
repaired the HClOꢀinduced oxidative stress. Then another dose of
PMA was added to the cells for 30 min, which resulted in the
increase of the intracellular fluorescence again (Figure 4d). The
data indicated probe MPhSeꢀBOD enabled to image for the redox
cycles between HClO and H2S in living cells. Coꢀstaining with
nuclear counterstain Hoechst (Figure 4e and 4f) revealed that the
subcellular location of the probe was in the cytoplasm of the
60
65
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80
15 Figure 4. Confocal fluorescence images of the redox cycles
between
85
HClO and H2S in RAW264.7 cells. Macrophage cells were incubated
with MPhSeꢀBOD (10 ꢁM) for 10 min and then treated with various
stimulants at 37 °C. Cell images were obtained using an excitation
wavelength of 488 nm and emission band from 500 nm to 600 nm. (a)
20 Control. (b) Probeꢀloaded cells incubated with PMA (1.5 ꢁg/mL) for 30
min. (c) Probeꢀloaded, PMAꢀtreated cells incubated with H2S (100 ꢁM)
for 10 min. (d) Probeꢀloaded, PMAꢀtreated, H2Sꢀincubated cells treated
with a second dose of PMA (3 ꢁg/mL) for 30 min. (e) Overlay of images
showing fluorescence from MPhSeꢀBOD and Hoechst dye. (f) Overlay of
25 brightꢀfield, MPhSeꢀBOD, and Hoechst dye images. Scale bar: 40 ꢀm.
90
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In summary, we have designed and synthesized a new
reversible
fluorescence probe MPhSeꢀBOD based on BODIPY platform containing
a selenide functional group. MPhSeꢀBOD can sensitively and selectively
detect HClO/H2S redox cycle in the solution and in living cells. MPhSeꢀ
30 BOD can be quickly oxidized by HClO with strong fluorescence increase.
The oxidation probe can be reduced by H2S to its original form along with
the decrease of the fluorescence to its starting level. Confocal microscopy
imaging in RAW264.7 cell lines shows that the probe has good living cell
permeability, and can monitor intracellular HClO/H2S redox cycle
35 replacement continuously.
100
105
110
115
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We are grateful to the National Science Foundation of China (21273234
and 21203192) and the National Basic Research Program of China
(2013CB834604) for financial support.
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