Irreversible inactivation of macrophage and brain nitric oxide synthase by L-N(G)-methylarginine requires NADPH-dependent hydroxylation

Article abstract of DOI:10.1021/jm00056a009

L-N(G)-Methylarginine (NMA) is an established mechanism-based inactivator of murine macrophage nitric oxide synthase (mNOS). In this report, NMA is shown to irreversibly inhibit both mNOS (k(inact) = 0.08 min^-1) and the recombinant constitutive