RNA modified uridines VII: Chemical synthesis and initial analysis of tRNA D-loop oligomers with tandem modified uridines

Article abstract of DOI:10.1080/15257779508014659

The first chemical synthesis of oligoribonucleotides with adjacent and significantly different modified uridines, the hydrophobic dihydrouridine, D, and the hydrophilic 3-[3-(S)-amino-3-carboxypropyl]-uridine, (acp)³U, is reported. The trimers Dp-acp³UpA, Up-acp³UpA, DpUpA and UpUpA, and the dimer Dp-acp³U were synthesized and initial structural analysis performed. The synthesis included a combination of protecting groups that is generally applicable to oligonucleotide syntheses in combination with various 2'OH protecting groups. The protecting groups did not cause racemization of the amino acid residue of (acp)³U during deprotection. The assignment of all ¹H NMR resonances of modified nucleoside-containing oligoribonucleotides includes heteronuclear one and two dimensional NMR.

Full text of DOI:10.1080/15257779508014659

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RNA Modified Uridines VII: Chemical
Synthesis and Initial Analysis of
tRNA D-Loop Oligomers with Tandem
Modified Uridines
Barbara Nawrot a , Andrzej Malkiewicz a c , Wanda S. Smith b
Hanna Sierzputowska-Gracz b & Paul F. Agris b d
,
a Institute of Organic Chemistry, Technical University , Lodz,
Poland
b Biochemistry Department , North Carolina State University ,
Raleigh, NC, USA
c Department of Biochemistry , North Carolina State University ,
Raleigh, NC, 27695-7622
d Department of Biochemistry , North Carolina State University ,
Raleigh, NC, 27695-7622
Published online: 24 Sep 2006.
To cite this article: Barbara Nawrot , Andrzej Malkiewicz , Wanda S. Smith , Hanna
Sierzputowska-Gracz & Paul F. Agris (1995) RNA Modified Uridines VII: Chemical Synthesis and
Initial Analysis of tRNA D-Loop Oligomers with Tandem Modified Uridines, Nucleosides and
Nucleotides, 14:1-2, 143-165, DOI: 10.1080/15257779508014659
To link to this article: http://dx.doi.org/10.1080/15257779508014659
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