Synthesis and evaluation of oligodeoxynucleotides containing 4'-C-substituted thymidines

Article abstract of DOI:10.1016/0040-4039(96)01432-3

4'-C-Hydroxymethylthymidine was converted to 4'-C-methoxymethylthymidine and 4'-C-aminomethylthymidine, which were incorporated into oligodeoxynucleotides by phosphoramidite chemistry. The modified oligonucleotides exhibit excellent hybridization and significant improvement in stability to snake venom phosphodiesterase.