Synthesis of enantiomerically pure phosphorothiolate assay substrate for phosphatidylinositol-specific phospholipase C

Article abstract of DOI:10.1016/S0960-894X(97)00197-2

An optimized synthesis of enantiopure (2R)-1,2-dioctanoyloxy- and (2R)-1,2-dipalmitoyloxypropane-thiophospho-(1D-myo- inositol) is reported starting from R-glycidol and the readily available 1D-1-tert-butyldiphenylsilyl-myo-inositol. The key synthesis of the phosphothiolester bond is carried out by the phosphoramidite chemistry.