
Bioscience, Biotechnology and Biochemistry p. 474 - 478 (1995)
Update date:2022-09-26
Topics:
Muro
Watanabe
Sugihara
Shimada
Nagao
Takenishi
Tominaga
Streptomyces limosus was selected because it secreted a novel protease that catalyzed the synthetic reaction forming Pro-Pro-Pro from Pro-Pro. The protease was purified to an electrophoretically homogeneous state and an activity of more than about 20,000-fold that of the culture broth. The molecular mass of the enzyme was estimated to be 50 kDa by SDS-polyacrylamide gel electrophoresis. The enzyme was most active in alkaline pH for the synthetic reaction producing Pro-Pro-Pro from Pro-Pro, although for the hydrolytic reaction forming proline it was most active in neutral pH. The enzyme was inhibited by 1,2-epoxy-3-(p-nitrophenoxy)propane (EPNP) and diazoacetyl-DL-norleucine methyl ester (DAN). It can be considered that this enzyme belongs to the class of aspartic proteases. The substrate specificity indicates that this enzyme has a strong affinity for proline as a N-terminal amino acid of peptides.
View MoreContact:+86-25-83719363
Address:106-7 Chunnan Rd, Chunxi Town, Gaochun, Nanjing, China
Contact:--
Address:80G, No.1 Building, Guodu Development Mansion, No. 182 Zhaohui Road, Hangzhou City, Zhejiang Province,China.
WUHU HUAHAI BIOLOGY ENGINEERING CO LTD
Contact:+86-553-3836920
Address:7/F NO.82 LAODONG ROAD WUHU CHINA
WUXI HONOR SHINE CHEMICAL CO.,LTD
Contact:+86-510-83593312
Address:No.1699 Huishan avenue,Huishan district,Wuxi ,Jiangsu,China,214177.(Wuxi Huishan Ecomonic Develop Zone )
TIANJIN ZHONGXIN CHEMTECH CO.,LTD.
Contact:86-022-66880623
Address:FINANCIAL STREET WEST BLK 7, #308, NO.52 XINCHENG WEST ROAD, TEDA, TIANJIN, P.R.CHINA
Doi:10.1055/s-0030-1259041
(2010)Doi:10.1134/S1070328410120079
(2010)Doi:10.1016/j.ica.2010.09.045
(2011)Doi:10.1002/adsc.201000592
(2010)Doi:10.1002/ejoc.201001509
(2011)Doi:10.1016/0008-6215(89)85021-9
(1989)