Synthesis, hybridization, and nuclease resistance properties of 2'-O- aminooxyethyl (2'-O-AOE) modified oligonucleotides

Article abstract of DOI:10.1016/S0040-4039(98)02498-8

The novel RNA mimic 2'-O-AOE has been incorporated into antisense oligonucleotides. This 2'-O-modification significantly enhances hybridization against target RNA, and furthermore, exhibits specificity for RNA over DNA. The nuclease resistance (SVPD) of 2'-O-AOE modified phosphodiester oligonucleotides is significantly higher than the unmodified DNA and comparable to the 2'-O-MOE oligonucleotides.