Synthesis of partially-deuterated 2'-deoxyribonucleoside blocks and their incorporation into an oligo-DNA for simplification of overcrowding and selective enhancement of resolution and sensitivity in the 1H-NMR spectra

Article abstract of DOI:10.1016/S0040-4020(98)00905-3

The chemical synthesis of appropriately protected partially-deuterated 2'(R/S),3',5'(R/S)-²H₃-2'-deoxyribonucleoside blocks [~43 atoms % ²H at C5'(R), ~57 atom % ²H at C5'(S); ~15 atom % ²H at C2'(R), ~85 atom % ²H at C2'(S) and >99 atom % ²H at C3'] is reported. The availability of these deuterium labelled blocks on large scale has enabled the chemical assemblage of the deuterio isotopomeric 12mer [d(C¹G²C³G⁴A⁵A⁶T⁷T⁸C⁹G¹⁰C¹¹G¹²)]₂ DNA duplex by standard solid-phase synthesis protocol in order to demonstrate the usefulness of the new 'NMR-window III' approach.