Bioorganic and Medicinal Chemistry Letters p. 1133 - 1136 (1999)
Update date:2022-08-05
Topics:
Rukavishnikov, Aleksey V.
Zaikova, Tatiana O.
Birrell, G. Bruce
Keana, John F. W.
Griffith, O. Hayes
The synthesis of a fluorogenic substrate for mammalian phosphoinositide- specific phospholipase C is described. The substrate, based on the widely used fluorescein molecule, is a water-soluble substrate analog of phosphatidylinositol-4-phosphate. The fluorogenic substrate 2 is shown to be a sensitive substrate for human PI-PLC-δ1 in a continuous assay.
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Doi:10.1039/j39690001758
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