Identification of Butyryl Derivatives of Cyclic Nucleotides by Positive Ion Fast Atom Bombardment Mass Spectrometry and Mass-analysed Ion Kinetic Energy Spectrometry

Article abstract of DOI:10.1002/oms.1210240827

The syntheses of the dibutyryl derivatives of the 3',5',-cyclic monophosphates of adenosine, guanosine and cytidine are described.The fast atom bombardment mass spectra of these compounds are discussed, together with the mass-analysed ion kinetic energy spectra of their protonated molecular ions and of diagnostic fragments.A protocol for the identification of the derivatives is reported which includes criteria for confirming retention of the cyclic phosphodiester moiety, substitution of both heterocyclic base and ribose ring, and butyrylation of the 2',-O-position.The origins of significant fragments in the spectra are discussed.