Carbohydrate Research p. 20 - 25 (2019)
Update date:2022-08-03
Topics:
Fujimori, Tae
Matsuda, Ryoko
Suzuki, Mami
Takenaka, Yuto
Kajiura, Hiroyuki
Takeda, Yoichi
Ishimizu, Takeshi
UDP-apiose, a donor substrate of apiosyltransferases, is labile because of its intramolecular self-cyclization ability, resulting in the formation of apiofuranosyl-1,2-cyclic phosphate. Therefore, stabilization of UDP-apiose is indispensable for its availability and identifying and characterizing the apiosyltransferases involved in the biosynthesis of apiosylated sugar chains and glycosides. Here, we established a method for stabilizing UDP-apiose using bulky cations as counter ions. Bulky cations such as triethylamine effectively suppressed the degradation of UDP-apiose in solution. The half-life of UDP-apiose was increased to 48.1 ± 2.4 h at pH 6.0 and 25 °C using triethylamine as a counter cation. UDP-apiose coordinated with a counter cation enabled long-term storage under freezing conditions. UDP-apiose was utilized as a donor substrate for apigenin 7-O-β-D-glucoside apiosyltransferase to produce the apiosylated glycoside apiin. This apiosyltransferase assay will be useful for identifying genes encoding apiosyltransferases.
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Doi:10.1016/j.molcatb.2015.05.020
(2015)Doi:10.1016/j.tet.2015.12.035
(2016)Doi:10.1016/S0008-6215(01)00132-X
(2001)Doi:10.1021/acs.orglett.9b00983
(2019)Doi:10.1139/v71-619
(1971)Doi:10.1016/j.ejmech.2019.01.048
(2019)