Studies on N-Glycopeptides. II. Separation of α- and β-Amide of N-(L-Aspartyl)-β-D-glucopyranosylamine Obtained via N-(Benzyloxycarbonyl)-L-aspartic Anhydride

Article abstract of DOI:10.1246/bcsj.57.3167

A mixture of N-(L-α-aspartyl)- and N-(L-β-aspartyl)-β-D-glucopyranosylamine was obtained by the condensation of N-(benzyloxycarbonyl)-L-aspartic anhydride with β-D-glucopyranosylamine.Attempts to separate the mixture by using cation-exchange chromatography, a copper(II) complex, and fractional recrystallization were carried out.These separations gave good yields of the desired β-amine.