Synthesis of deuterium-labelled coenzyme Q10

Article abstract of DOI:10.1002/jlcr.587

Deuterium-labelled coenzyme Q₁₀ ([2-CD₃-1′-CD₂]coenzyme Q₁₀, coenzyme Q₁₀-d₅) was synthesized by condensation of 2,3-dimethoxy-[5-CD₃]methyl-1, 4-hydroquinone with [1-CD₂]decaprenol. Five positions were selected for deuteration as replacement at these positions allowed examination of every step of the synthesis. This examination was carried out by a combination of ¹H- and ¹³C-nuclear magnetic spectrometry and mass spectrometry. Further, these positions have been proved to be metabolically stable. This reagent makes simultaneous quantification of the source of coenzyme Q₁₀ (exogenously supplied or endogenously supplied) possible in biological samples by measurements on gas chromatography-mass spectrometry. Copyright