The search for biochemical photoprobes. III. The photoreactions of 4-nitroveratrole and 2-fluoro-4-nitroanisole with bovine pancreatic ribonuclease A and with model nucleophiles

Article abstract of DOI:10.1016/S0040-4020(01)85819-1

The photoreactions of 4-nitroveratrole (NVT) and 2-fluoro-4-nitroanisole (FNA) with bovine pancreatic ribonuclease A (RNAase A) and with model nucleophiles are described. From them it is concluded that 2-fluoro-4-nitrophenyl ethers constitute improved alternative biochemical photoprobes, specially for proteins where a number of good nucleophiles are present.