3-Phenanthryl Chalcones
463
24 hrs, the plates were visually examined and the diameter values of the zones of inhib-
ition were measured. Triplicate results were recorded by repeating the same procedure.
Controls showed luxuriant growth.
Antifungal Sensitivity Assay
Antifungal sensitivity assays were performed using the Kirby-Bauer14 disc diffusion tech-
nique. Potato dextrose agar medium was prepared and sterilized. It was poured into the
plates already containing 1 mL of the fungal species (10 mg of the fungal sample dissolved
in 100 ml of sterile distilled water). Whatman No.1 5 mm discs were impregnated with the
test solution, prepared by dissolving 15 mg of the enone in 1 mL of DMSO solvent. The
medium was allowed to solidify and kept for 24 hrs. Then the plates were visually exam-
ined and the diameter values of the zones of inhibition were measured. Triplicate results
were recorded by repeating the same procedure. Controls showed luxuriant growth.
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