First synthesis of H-phosphonate oligonucleotides bearing N-unmodified bases

Article abstract of DOI:10.1016/S0040-4039(98)02481-2

Oligodeoxyribonucleotides bearing H-phosphonate internucleotidic linkages and unmodified nucleobases were synthesized for the first time by the new H-phosphonate method using N-unprotected monomers. The 6-hydroxyhexyl phosphonates at both the 5' and 3' ends were found to be highly effective for stabilization of the H-phosphonate oligonucleotides. Solid-phase synthesis of H-phosphonate oligodeoxyribonucleotides containing dA, dC, dG, and T was achieved.