Studies related to in vivo C-H activation: Synthesis and influence of 8,8- and 11,11-dimethyl oleic and 11,11-dimethyl linoleic acids on Δ12- desaturation of C. sorokiniana

Article abstract of DOI:10.1016/S0040-4020(98)01166-1

The synthesis of three acids was achieved using the Wittig reaction in order to study their in vivo influence on the Δ12-desaturase of Chlorella sorokiniana. It was shown that the introduction of two methyls near the double bond prevent the desaturation of these exogenous acids while they seem to be accurately incorporated. This functionality could be of interest for the design of new thiaoleic acids as probes of the different oxidation processes.