METHYLATION OF DEOXYNUCLEOTIDES WITH TRIMETHYL PHOSPHATE IN AQUEOUS PHASE.

Article abstract of DOI:10.1246/bcsj.56.1826

Action of trimethyl phosphate on thymidine 5 prime -phosphate, deoxycytidine 5 prime -phosphate, deoxyadenosine 5 prime -phosphate, and deoxyguanosine 5 prime -phosphate at 37-60 degree C, and pH 7-10 gave their methyl esters and base-methylated derivatives. Esterification took place in all deoxyribonucleotides to the almost same extent and its rate was enhanced by the reaction temperature, while being independent of pH in the range 7-10. On the other hand, methylation of the base moieties was affected by both the reaction temperature and pH of the reaction medium.