ELISA for PBDEs
J. Agric. Food Chem., Vol. 53, No. 10, 2005 3847
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analysis also explained why BDE-99 showed slightly improved
binding for antibody I-KLH. These results demonstrated the
importance of the linker position. The decreased competition
when BDE-153 and BDE-183 were used as competing com-
pounds was compatible with the model. The results also
indicated the coating antigen played an important role in
determining competition with the interacting ligand.
ABBREVIATIONS USED
BSA, bovine serum albumin; BDE, polybrominated diphenyl
ethers; BFR, brominated flame retardant; EDC, 1-ethyl-3-(3-
dimethylaminopropyl)carbodiimide; ELISA, enzyme-linked im-
munosorbent assay; KLH, keyhole limpet hemocyanin; MES,
2-[N-(morpholino)ethanesulfonic acid]; OD, optical density;
PBS, phosphate-buffered saline; PBST, phosphate-buffered
saline plus 0.05% Tween 20; TMB, 3,3′,5,5′-tetramethyl-
benzidine.
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ACKNOWLEDGMENT
We thank Montgomary Botschner, Jason E. Holthusen, Amy
M. McGarvey, JoLyn M. Gilbertson, and Habiba E. Vaghoo
for technical assistance. We thank Dr. Gregory Cook for
assistance in the AM1 calculations and Ulrika O˜ rn and Go˜ran
Marsh for synthesis of the PBDE congeners.
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characterization of polybrominated diphenyl etherssUnlabeled
and radiolabeled tetra-, penta-, and hexabromodiphenyl ethers.
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Received for review December 17, 2004. Revised manuscript received
February 23, 2005. Accepted February 25, 2005.
JF047863M