Synthesis and phytotoxic activity of new pyridones derived from 4-hydroxy-6-methylpyridin-2(1H)-one

Article abstract of DOI:10.3390/molecules14124973

Commercial dehydroacetic acid was converted into 4-hydroxy-6-methylpyridin- 2(1 H)-one (3), which was then condensed with several aliphatic aldehydes to produce seven new title compounds in variable yields (35-92%). Reaction of 3 with α,β-unsaturated aldehydes resulted in the formation of condensed pyran derivatives 4g′ and 4h′. A mechanism is proposed to explain the formation of such compounds. The effects of all methylpyridin-2(1 H)-one derivatives on the development of the dicotyledonous species Ipomoea grandifolia and Cucumis sativus and the monocotyledonous species Sorghum bicolor were evaluated. At the dose of 6.7 × 10^-8 mol a.i./g substrate the compounds showed some phytotoxic selectivity, being more active against the dicotyledonous species. These compounds can be used as lead structures for the development of more active phytotoxic products.

Full text of DOI:10.3390/molecules14124973

Molecules 2009, 14, 4973-4986; doi:10.3390/molecules14124973
OPEN ACCESS
molecules
ISSN 1420-3049
www.mdpi.com/journal/molecules
Article
Synthesis and Phytotoxic Activity of New Pyridones
Derived from 4-Hydroxy-6-Methylpyridin-2(1H)-one
Antonio Jacinto Demuner 1, Vania Maria Moreira Valente 1, Luiz Cláudio Almeida Barbosa 1,2,*,
Akshat H. Rathi 2, Timothy J. Donohoe 2 and Amber L. Thompson 2
1
Department of Chemistry, Federal University of Vicosa, Av. P. H. Rolfs, s/n, 36570-000, Vicosa,
MG, Brazil; E-Mails: ademuner@ufv.br (A.J.D.); vvalente@ufv.br (V.M.M.V.)
2
Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, UK;
E-Mails: akshat.rathi@chem.ox.ac.uk (A.H.R.); timothy.donohoe@chem.ox.ac.uk (T.J.D.);
amber.thompson@chem.ox.ac.uk (A.L.T.)
* Author to whom correspondence should be addressed; E-Mail: lcab@ufv.br.
Received: 5 November 2009; in revised form: 24 November 2009 / Accepted: 30 November 2009 /
Published: 1 December 2009
Abstract: Commercial dehydroacetic acid was converted into 4-hydroxy-6-methylpyridin-
2(1H)-one (3), which was then condensed with several aliphatic aldehydes to produce seven
new title compounds in variable yields (35–92%). Reaction of 3 with α,β-unsaturated
aldehydes resulted in the formation of condensed pyran derivatives 4g’ and 4h’. A mechanism
is proposed to explain the formation of such compounds. The effects of all methylpyridin-
2(1H)-one derivatives on the development of the dicotyledonous species Ipomoea grandifolia
and Cucumis sativus and the monocotyledonous species Sorghum bicolor were evaluated. At
the dose of 6.7 × 10-8 mol a.i./g substrate the compounds showed some phytotoxic selectivity,
being more active against the dicotyledonous species. These compounds can be used as lead
structures for the development of more active phytotoxic products.
Keywords: pyridine; x-ray; phytotoxicity; weeds; herbicides
1. Introduction
Pyridin-2(1H)-ones are known to possess a range of biological activities such as analgesic,
antifungal, antimalarial, antiinflammatory, antibacterial, anti-HIV, phytotoxic, antitumoral and

Molecules 2009, 14
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antiviral properties [1–9]. Functionalized pyridin-2(1H)-ones have been used as versatile intermediates
in the synthesis of a wide range of nitrogen-containing heterocycles, such as pyridine, quinolizidine,
and indolizidine alkaloids [10,11]. Fluridone and sapinopyridione are representative examples of
pyridinones that possess phytotoxic activity [5,7]. This class of compounds has been used to formulate
lead compounds for the synthesis of new pyridiones [1,3,4,12,13], including the bis(pyridyl)methanes,
which present antitumoral activity [8,9]. Recently, we have been utilizing natural products as models
for the development of new compounds with phytotoxic activity [14–24]. In continuing our research in
this direction, we now report the synthesis of pyridone derivatives 4a-h and some studies on their
phytotoxic activities.
2. Results and Discussion
2.1. Synthesis of bis-pyridones
The hydrolysis of dehydroacetic acid 1 [25] with sulfuric acid afforded compound 2 in 86% yield.
Compound 2 was then reacted with aqueous ammonium hydroxide to produce the corresponding
pyridone 3 in 80% yield. Treatment of 3 under basic conditions in the presence of several aldehydes
resulted in the condensed compounds 4a-h in variable yields (Scheme 1).
Scheme 1. Reagents and conditions for the synthesis of pyridones 4a-h.
OH
O
OH
OH
R
OH
OH
iii
iv
i
ii
O
O
N
O
O
O
N
H
OO
N
H
H
3
1
2
4a
4b
4c
4d
4e
4f
R= H
CH3
92 %
35 %
56 %
0 %
56 %
75 %
0 %
CH2CH3
CH=CH2
Ph
CH2CH2Ph
CH=CHPh
C(CH3)=CHPh
4g
4h
0 %
Reagents and conditions: i) H2SO4 92% (5 eq), 130 °C, 10 minutes, 86%. ii) NH4OH(aq) (5 eq),
100 °C, 6h, 80%. iii) compound 3 (2 eq), ethanol/methanol (1:1 v/v), piperidine (0.15 eq), pyridine
(0.55 eq), 25 °C. iv) RCHO (1 eq).
It was found that formaldehyde reacted very quickly and efficiently, resulting in a 92% yield of the
condensed bis-pyridone 4a. In the cases of acetaldehyde and propanaldehyde the reactions were not
clean, giving the desired condensation compounds in much lower yields (4b, 35%; 4c, 56%) and
several side products were observed by TLC analysis of the crude reaction mixtures. To study the
influence on the biological activity of an unsaturated group at the methine carbon, acrolein was reacted
with compound 3 under the same conditions described. In this case the required bis-pyridone was not
isolated and only polymerized compounds were detect by ESI-MS and NMR of the crude reaction
mixture.

Molecules 2009, 14
4975
In order to further explore the Structure Activity Relationships (SAR), the preparation of condensed
bis-pyridone containing aromatic groups linked to the methine carbon bridge was attempted. Thus, the
reaction of benzaldehyde and 2-phenylpropanaldehyde with 3 resulted in the formation of the
corresponding bis-pyridones 4e and 4f in 56% and 75% yields, respectively. The structures of all
condensed products were determined by spectroscopic analysis. In the case of compound 4f, the
structure was also determined by X-ray analysis (Figure 1).
Figure 1. Single crystal structure of compound 4f. Atomic displacement parameters shown
at 50% probability and the disorder are omitted for clarity.
O26
O11
N12
N3
O1
O16
Unexpectedly the reaction of pyridione 3 with cinnamaldehyde and (E)-2-methylcinnamaldehyde
did not give the desired condensed products. In case of the cinnamaldehyde adduct the HR-MS
showed a peak at m/z =387.1301, corresponding to the molecular formula C21H20NaN2O4. The
13C-NMR spectrum showed signals corresponding to two CH3, one CH2, six CH, two C=O and seven
non-hydrogenated carbons. For the (E)-2-methylcinnamaldehyde adduct, the product isolated as a
white solid with HR-MS showed a peak at m/z = 276.0095 corresponding to the molecular formula
13
C16H15NaNO2. The C-NMR spectrum showed signals corresponding to two CH3, six CH, one C=O
and five non-hydrogenated carbons. Single crystal diffraction data were collected for both 4g’ and 4h’
yielding the structures shown in Scheme 2 and Figures 2 and 3. Both compounds were formed as a
racemic mixture. Formation of a compound similar to 4h’ has been reported by Lee et al. [26],
however in none of the published examples a compound with the structure of 4g’ was observed.
Although no mechanistic investigation was carried out, a pathway is proposed that leads to the
formation of 4g’ and 4h’ (Scheme 2). Initially, an aldol condensation of pyridone 3 with
cinnamaldehyde results in the intermediate (a). Depending on the nature of the R group (H or CH3) the
product formed in the next step is different. In case of cinnamaldehyde a 1,6-addition of a second
molecule of the pyridone resulting in the intermediate (c) occurs. This intermediate can be in
equilibrium with (d), which under basic conditions cyclises to yield 4g’. In case of
(E)-2-methylcinnamaldehyde, steric hindrance involving the methyl group and the oxygen favors the
formation of (b). This intermediate then undergoes a 3,3-sigmatropic rearrangement to give 4h’. This
rearrangement ensures that a second addition is impossible. At this point we should observe that

Molecules 2009, 14
4976
according to the proposed mechanism a concentration of pyridine could have some influence on the
results of the reactions, leading to different products, but this is open to further investigation.
Scheme 2. Proposed mechanism for formation of 4g’ and 4h’.
O
O
Ph
H
N
H
O
R
3
R= H or Me
-H2O
Ph
O
R
O
Ph
R
N
H
O
N
H
O
(a)
H
(b)
O
N
R=CH3
R=H
O
N
Ph
H
O
CH3
O
O
O
Ph
N
H
O
4h'
N
H
O
(c)
H
O
N
H
N
OH
O
O
B
O
O
O
OH
Ph
Ph
N
H
N
H
4g'
(d)

Molecules 2009, 14
Figure 2. Single crystal structure of compound 4g’. Atomic displacement parameters
4977
shown at 50% probability, one equivalent and the disordered DMSO are omitted for
clarity.
O26
N5
O24
N13
O9
O1
Figure 3. Single crystal structure of 4h’ showing the hydrogen bonding amide dimer.
Atomic displacement parameters are shown at 50% probability.
O17
N15’
O1
N15
O17’
2.2. Phytotoxicity assay
All the compounds were evaluated against S. bicolor, C. sativus and I. grandifolia, using sand as a
substrate. Although the compounds could have different adsorption properties on sand and this could
affect the biological activity, this aspect was beyond the scope of the current work. The effect of
compounds 4a-c, 4e-f, 4g’ and 4h’ on the radicle growth of Sorghum bicolor and Cucumis sativus was
evaluated at 5 × 10-4 mol L-1 concentration (Table 1). All the compounds caused less than 50%
inhibition of both tested plants. The most active compounds were 4g’ and 4h’, should be causing
around 45% inhibition of the root growth of S. bicolor and C. sativus.

Molecules 2009, 14
Table 1. Effects of compounds 4a-c and 4e-h (5 × 10-4 mol L-1) on the radicle growth of S.
4978
bicolor and C. sativus seedlings after 72 hours.
C. sativus
Compound
S. bicolor
inhibition (%)
27.1 Ba
inhibition (%)*
4a
4b
4c
27.6 Ca
31.2 BCa
30.2 BCa
36.5 ABCa
38.5 ABCa
41.0 ABa
45.3 Aa
23.9 Ba
26.9 Ba
18.9 Bb
25.0 Bb
44.3 Aa
44.3 Aa
4e
4f
4g’
4h’
* Mean values in the same column with the same capital letters are
not significant at P = 0.05% by Tukey’s test; Mean values in the
same line with the same small letter are not significant at
P = 0.05% by Tukey’s test.
Once all compounds showed some activity on the Petri dish test they were submitted to a 21 day
assay in a greenhouse using S. bicolor, C. sativus and the weed Ipomoea grandifolia as test plants
(Table 2).
Table 2. Effect of compounds 4a-c, e-h (6.7 × 10-8 mol a.i./g substrate) on the biomass
production of radicle growth of S. bicolor and C. sativus seedlings after 21 days.
S. bicolor
aerial part* roots
C. sativus
aerial part roots
I. grandifolia
aerial part roots
-
4a
4b
4c
4e
4f
22.3 CDc
31.8 BCc
51.6 Ab
37.4 ABb
42.6 Bb
63.7 Aa
63.5 Aa
34.9 BCa
38.7 BCa
38.6 BCb
27.5 Cc
65.0 BCa
80.8 Aa
64.4 Aa
49.8 Bb
68.4 Aa
32.8 Ca
45.6 BCa
56.8 ABa
49.3 Ba
38.6 DEb
45.5 CDb
72.8 Aa
32.8 Eb
44.3 CDEb
58.2 Bb
48.0 Ab
44.2 Ab
31.7 Bb
38.6 ABb
46.8 Ac
42.8 ABb
63.9 BCa
67.5 BCa
57.6 Ca
74.5 ABa
63.8 BCa
18.1 Db
40.2 ABa
42.7 ABb
38.1 Bb
4g’
4h’
55.3 BCa
* Mean values in the same column with the same capital letters are not significant at P = 0.05% by
Tukey’s test; Mean values in the same line with the same small letter are not significant at P =
0.05% by Tukey’s test.
In the case of S. bicolor compounds 4c, 4f and 4g’ caused higher inhibitory effect on the
development of the aerial parts (51.6%, 40.2% and 42.7%, respectively). On the other hand, 4b, 4c,
and 4g’ caused greatest root inhibition development (48.0%, 44.2% and 46.8%, respectively). The
results observed for compound 4g’ confirm its higher activity found in the Petri dish test in relation to
the other compounds.
For C. sativus, a dicotyledonous species, compounds 4b and 4c most severely inhibited the
development of the aerial parts (63.7% and 63.5%, respectively). All the other compounds, including
the pyrans 4g’ and 4h’, caused less than 50% inhibition on the aerial parts. The development of the
roots of C. sativus was most affected by compounds 4b (80.8% inhibition) and 4g’ (74.5%). All the

Molecules 2009, 14
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other compounds were also very active against this dicotyledonous species, causing increases
inhibition of the development of their roots compared to the aerial parts.
Having confirmed the phytotoxicity of the pyridone derivatives against the monocotyledon and
dicotyledonous test species, their effect on the development of the important dicotyledonous weed
species I. grandifolia were also evaluated. For this species, compounds 4a, 4c and 4g’ inhibited the
development of the aerial part by 64.4%, 68.4% and 56.8%, respectively. Compound 4c was by far the
most effective in inhibiting the roots of I. grandifolia 72.8%), followed by compounds 4g’ (58.2%
inhibition) and 4h’ (55.3%). All the remaining compounds inhibited the development of the roots of I.
grandifolia less than 50%.
The compound 4g’ serendipitously emerged as the most potent inhibitor, showing moderate to high
inhibition (38.6% to 74.5%) against all the species tested. Although a clear structure-activity
relationship cannot be secured from these data, it is evident that the pyran ring is somehow associated
with the activity. It may be concluded that presence of one less pyridone moiety in the molecule does
not reduce the phytotoxicity, but rather increases it and that the presence of an aromatic ring at any
position (closer or farther from the pyridone ring) encourages higher activity than an alkyl substituent.
3. Experimental
3.1. General
All the chemicals were purchased from Sigma Aldrich (Milwaukee, WI, USA) and used without
1
purification. The H- and 13C-NMR spectra were recorded on a Varian Mercury 300 instrument
(300 MHz and 75 MHz respectively), using deuterated DMSO as a solvent and tetramethylsilane
(TMS) as internal standard (δ = 0). IR spectra were obtained using a Perkin Elmer Paragon 1000 FTIR
spectrophotometer, using potassium bromide (1% v/v) disks, scanning from 600 to 4000 cm-1. Low-
resolution mass spectra were recorded on a Fisons Platform instrument under ESI conditions.
Chemical ionisation accurate mass spectra were recorded on an Apex III FT-ICR-MS spectrometer
with high resolution (resolution = 100,000 FWHM). The lock used for calibration was
chloropentafluorobenzene. Values quoted are reported as a ratio of mass to charge in Daltons. Melting
points are uncorrected and were obtained from MQAPF-301 melting point apparatus (Microquimica,
Brazil). Analytical thin layer chromatography analysis was conducted on aluminum backed precoated
silica gel plates. Column chromatography was performed over silica gel (60–230 mesh).
3.2. Synthesis
3.2.1. Synthesis of 4-hydroxy-6-methyl-2H-pyran-2-one (2)
Dehydroacetic acid 1 (1 mmol) and 92% sulfuric acid aqueous solution (5 mmol) were placed in a
25 mL flask and the mixture was heated to 130 °C for 10 minutes. While still warm, the mixture was
poured into a beaker containing chopped ice. The resulting precipitate formed was filtered off and
washed with cold water, leading to the isolation of 4-hydroxy-6-methylpyran-2-one (2) as a white solid
in 86% yield. Mp 186.5–187.7 °C; IR: 3,440, 3,097, 2,962, 2,821, 2,733, 2,622, 1,717, 1,657, 1,626,
1,586, 1,540, 1,509, 1,493, 1,344, 1,303, 1,256, 1,191, 1,149, 1,042, 989, 878, 839, 814, 591, 526, and

Molecules 2009, 14
4980
1
498 cm-1; H-NMR (DMSO-d6) δ: 2.13 (d, 3H, J = 0.3 Hz, CH3), 5.19 (d, 1H, J = 2.1 Hz, H3), 5.93
(m, 1H, H5 ), 11.58 (bs, 1H, OH); 13C-NMR (DMSO-d6) δ: 20.1 (6-CH3), 88.8 (C3), 100.9 (C5), 164.0
(C6), 164.6 (C4), 171.2 (C2); MS, m/z (%) 126 (M+, 33), 111 (15), 98 (70), 85 (33), 69 (100), 55 (25).
3.2.2. Synthesis of 4-hydroxy-6-methylpyridin-2(1H)-one (3)
4-Hydroxy-6-methylpyran-2-one (2, 1 mmol) and 28% ammonium hydroxide (5 mmol) were added
under stirring to a 25 mL flask. The mixture was heated to 100 °C for 6 h, after which it was cooled in
an ice bath and the resultant white solid was filtered off and washed with cold water to provide
4-hydroxy-6-methylpyridin-2-one (3) in 80% yield; Mp > 300 °C; IR: 3,445, 3,268, 3,091, 2,894,
2,796, 2,716, 1,653, 1,634, 1,603, 1,550, 1,400, 1,381, 1,352, 1,267, 1,232, 1,175, 1,042, 903, 842,
1
830, 627, 595, and 535 cm-1; H-NMR (DMSO-d6) δ: 2.06 (d, 3H, J = 0.3 Hz, 6-CH3), 5.32 (d, 1H,
J = 2.1 Hz, H3), 5.58 (m, 1H, H5), 10.97 (bs, 1H, OH), 10.41 (bs, 1H, NH); 13C-NMR (DMSO-d6): δ
19.1 (6-CH3), 96.4 (C3), 99.0 (C5), 146.6 (C6), 165.53 (C2), 168.3 (C4); MS, m/z (%) 125 (M+, 77),
96 (22), 84 (100), 69 (17), 68 (17), 55 (16).
3.2.3. General method of preparation of bis(pyridyl)methanes 4a-h
To a 100 mL round-bottomed flask, 95% ethanol (20 mL), methanol (20 mL), 4-hydroxy-6-
methylpyridin-2-one (3, 1.5 mmol), piperidine (15 μL) and pyridine (45 μL) were added. The reaction
mixture was kept under stirring for 5 minutes at 25 °C. The corresponding aldehyde (1 mmol) was
added and the mixture kept under reflux. On completion of the reaction, as determined by TLC
analysis, the volume of solvent was reduced under reduced pressure in a rotatory evaporator and the
precipitate formed was filtered and washed with methanol, followed by ethyl ether. This led to the
formation of 4a-h in high purity, without need of any further purification.
3,3’-Methylenebis(4-hydroxy-6-methylpyridin-2(1H)-one) (4a). Reflux 1.5 h, White solid; yield: 92%;
Mp > 300 °C; IR: 3,445, 3,284, 3,059, 3,023, 2,899, 2,644, 1,640, 1,568, 1,442, 1,389, 1,368, 1,267,
1
1,196, 1,132, 910, 835, 805, 611, and 546 cm-1; H-NMR (DMSO-d6) δ: 2.10 (s, 6H, 6-CH3 and
6’-CH3), 3.41 (s, 2H, H7), 5.86 (s, 2H, H5 and H5’), 11.74 (s, 2H, H1 and H1’), 12.11 (bs, 1H, 4-OH
and 4’-OH); 13C-NMR (DMSO-d6) δ: 18.7 (C7), 18.9 (6-CH3 and 6’-CH3), 102.1 (C5 and C5’), 109.1
(C3 and C3’), 144.3 (C6 and C6’), 165.6 (C2 and C2’), 167.0 (C4 and C4’); HRMS (ESI TOF-MS):
Calcd. for C13H14N2O4 262.0954; found: 262.0951.
3,3'-(Ethane-1,1-diyl)bis(4-hydroxy-6-methylpyridin-2(1H)-one) (4b). Reflux 90 h, White solid; yield:
35%; Mp > 300 °C; IR: 3,446, 3,264, 2,891, 2,610, 1,644, 1,558, 1,451, 1,390, 1,335, 1,265, 1,198,
1,165, 915, 816, 641, and 553 cm-1; 1H-NMR (DMSO-d6) δ: 1.58 (d, 3H, J = 7.5 Hz, H8), 2.09 (s, 6H,
6-CH3 and 6’-CH3), 4.49 (q, 1H, J = 7.5 Hz, H7), 5.81 (s, 2H, H5 and H5’), 11.63 (s, 2H, H1 and H1’);
13C-NMR (DMSO-d6) δ: 15.3 (C8), 18.7 (6-CH3 and 6’-CH3), 25.3 (C7), 102.7 (C5 and C5’), 112.9
(C3 and C3’), 144.0 (C6 and C6’), 166.5 (C2 and C2’), 166.7 (C4 and C4’); HRMS (ESI TOF-MS):
Calcd. for C14H16N2O4 276.1110; found: 276.1110.

Molecules 2009, 14
4981
3,3´-(Propane-1,1-diyl)bis(4-hydroxy-6-methylpyiridin-2(1H)-one) (4c). Reflux 90 h, White solid;
yield 56%; Mp > 300 °C; IR: 3,447, 3,057, 2,955, 2,872, 2,622, 1,637, 1,460, 1,393, 1,361, 1,265,
1,252, 1,193, 835, 817, 624, and 557 cm-1; 1H-NMR (DMSO-d6) δ: 0.73 (t, 3H, J = 7.2 Hz, CH3), 2.03-
2.20 (m, 8H, H8, 6-CH3 and 6’-CH3), 4.18 (t, 1H, J = 8.1 Hz, H7), 5.81 (s, 2H, H5 and H5’), 11.60 (s,
2H, H1 and H1’); 13C-NMR (DMSO-d6) δ: 13.9 (C9), 18.7 (6-CH3 and 6’-CH3), 21.4 (C8), 33.4 (C7),
102.7 (C5 and C5’), 111.6 (C3 and C3’), 144.2 (C6 and C6’), 166.1 (C2 and C2’), 166.2 (C4 and C4’);
HRMS (ESI TOF-MS): Calcd. for C15H18N2O4 290.1267; found: 290.1263.
3,3'-(Phenylmethylene)bis(4-hydroxy-6-methylpyridin-2(1H)-one) (4e). Reflux 40 h, White solid;
yield: 56%; Mp > 300 °C; IR: 3,445, 3,280, 3,062, 2,898, 1,632, 1,453, 1,394, 1,361, 1,284, 1,192,
924, 817, 772, and 583 cm-1; 1H-NMR (DMSO-d6) δ: 2.15 (s, 6H, 6-CH3 and 6’-CH3), 5.87 (bs, 3H, H-
7, H5 and H5’), 6.99 (d, 2H, J = 7.5 Hz, H2” and H6”), 7.12 (t, 1H, J1 = J2 = 7.5 Hz, H4”), 7.20 (t, 2H,
J = 7.5 Hz, H3” and H5”), 11.69 (s, 2H, H1 and H1’), 12.31 (bs, 1H, 4-OH and 4’-OH); 13C-NMR (75
MHz, DMSO-d6) δ: 18.9 (6-CH3 and 6’-CH3), 34.5 (C7), 102.5 (C5 and C5’), 110.2 (C3 and C3’),
126.0 (C4”), 126.9 (C2” and C6”), 128.5 (C3” and C5”), 139.6 (C1”), 144.8 (C6 and C6’), 167.7 (C2
and C2’), 167.5 (C4 and C4’); HRMS (ESI TOF-MS): Calcd. for C19H18N2O4 338.1267; found:
338.1265.
3,3'-(3-Phenylpropane-1,1-diyl)bis(4-hydroxy-6-methylpyridin-2(1H)-one) (4f). Reflux 120 h, White
solid; yield 75%; Mp 292.5–293.4 °C; IR: 3,446, 3,284, 3,058, 3,042, 3,024, 2,905, 2,622, 1,635,
1,559, 1,455, 1,437, 1,396, 1,257, 1,177, 816, 697, and 552 cm-1; 1H-NMR (DMSO-d6) δ: 2.10 (s, 6H,
6-CH3 and 6’-CH3), 2.4 (m, 2H, H8), 2.48 (m, 2H, H9), 4.31 (t, 1H, J = 7.5 Hz, H7), 5.83 (s, 2H, H5
and H5’), 7.06-7.24 (m, 5H, H2”, H3”, H4”, H5” and H6”), 11.65 (s, 2H, NH-1 and NH-1’), 11.86 (s,
13
2H, 4-OH and 4’-OH); C-NMR (DMSO-d6) δ: 18.8 (6-CH3 and 6’-CH3), 30.7 (C8), 30.8 (C7), 35.0
(C9), 102.7 (C5 and C5’), 111.5 (C3 and C3’), 126.3 (C4”), 128.9 (C3” and C5”), 129.0 (C2” and
C6”), 137.5 (C1”), 142.5 (C6 and C6’), 167.1 (C2 and C2’), 167.2 (C4 and C4’); HRMS (ESI+)
C21H22N2NaO4 (MNa+) required 389.1472, found 389.1479.
(±)-2-(4-Hydroxy-6-methyl-2-oxo-1,2-dihydropyridin-3-yl)-7-methyl-4-phenyl-3,4-dihydro-2H-
pyrano-[3,2-c]pyridin-5(6H)-one (4g’). Reflux 140 h, White solid; yield 57%; Mp 254.8–255.6 °C;
IR: 3,418, 3,132, 3,058, 2,932, 1,616, 1,579, 1,455, 1,390, 1,363, 1,321, 1,260, 1,181, 1,155, 1,113,
1
1,039, 971, 872, 821, 765, 701, 663, and 598 cm-1; H-NMR (DMSO-d6) δ: 1.66 (d, 1H, J = 13.8 Hz,
H8), 2.02 (s, 3H, 6-CH3), 2.09 (s, 3H, 6’-CH3), 3.10 (dt, 1H, J = 13.8, 4.8 Hz, H8’), 4.01 (d, 1H, J =
4.8 Hz, H7), 5.12 (dd, 1H, J = 12.3, 1.8 Hz, H9), 5.62 (s, 1H, H5), 5.65 (s, 1H, H5’), 7.08 (d, 2H, J =
7.2 Hz, H2” and H6”), 7.16 (t, 1H, J = 7.2 Hz, H4”), 7.26 (t, 2H, J = 7.2 Hz, H3” and H5”), 10.44 (s,
13
1H, 4’-OH); 11.02 (s, 2H, NH-1 and NH-1’); C-NMR (DMSO-d6) δ: 19.0 (6-CH3), 19.1 (6’-CH3),
32.5 (C8), 35.4 (C7), 67.2 (C9), 98.5 (C5), 98.7 (C5’), 104.6 (C3), 105.5 (C3’), 126.4 (C4”), 128.6
(C2” and C6”), 128.7 (C3” and C5”), 143.8 (C1”), 146.4 (C6 and C6’), 164.0 (C2), 164.3 (C2’), 164.9
(C4), 166.4 (C4’); HRMS (ESI+) C21H20N2NaO4 (MNa+) required 387.1315, found 387.1301.
(±)-3,7-Dimethyl-2-phenyl-2H-pyrano[3,2-c]pyridin-5(6H)-one (4h'). Reflux 190 h, White solid; yield
49%; Mp 222.4–223.3 °C; IR: 3,446, 3,058, 2,963, 2,897, 2,746, 1,669, 1,647, 1,630, 1,601, 1,565,

Molecules 2009, 14
4982
1,485, 1,453, 1,394, 1,263, 1,251, 1,145, 1,035, 951, 907, 869, 803, 770, 763, 696, 663, 649, 627, and
539 cm-1; 1H-NMR (DMSO-d6) δ: 1.61 (s, 3H, 8-CH3), 2.04 (s, 3H, 6-CH3), 5.59 (s, 1H, H5), 5.77 (s,
1H, H7), 6.40 (s, 1H, H9), 7.33-7.37 (m, 5H, H2’, H3’, H4’, H5’ and H6’), 11.20 (s, 1H, NH-1); 13C-
NMR (DMSO-d6) δ: 19.2 (6-CH3), 20.0 (8-CH3), 81.0 (C7), 98.0 (C5), 104.4 (C3), 114.5 (C9), 127.0
(C8), 128.1 (C2’ and C6’), 129.4 (C3’ and C5’), 129.5 (C4’), 139.5 (C1’), 145.6 (C6), 160.2 (C2),
161.3 (C4); HRMS (ESI+) C16H15NNaO2 (MNa+) required 276.0995, found 276.0995.
3.2.4. Single crystal structure determination
The crystal structures of 4f, 4g’ and 4h’ were determined from single crystal X-ray diffraction data
collected at 150 K with an Oxford Cryosystems Cryostream N2 open-flow cooling device [27]. Data
were collected using an Enraf-Nonius KappaCCD diffractometer (Mo-Kα radiation (λ = 0.71073 Å)
and processed using the DENZO-SMN package [28], including inter-frame scaling (which was carried
out using SCALEPACK within DENZO-SMN). The structures were solved using SIR92 [29].
Refinement was carried out using full-matrix least-squares within the CRYSTALS suite [30] on F2. A
small amount of disorder was present in 4f (alkyl bridge) and 4g’ (disordered DMSO) which was
modeled as detailed in the supplementary information (CIF). Refinement results are included in Table
3 and full crystallographic data have also been deposited with the Cambridge Crystallographic Data
Centre, CCDC 755517 – 755519. Copies of these data can be obtained free of charge from The
Cambridge Crystallographic Data Centre via www.ccdc.cam.ac.uk/data_request/cif.
Table 3. Crystal data and structure refinements for compounds 4f, 4g’ and 4h’.
4f
4g’
4h’
Chemical Formula
Fw
C21H22N2O4
366.41
C21H20N2O4·0.421(C2H6OS) C16H15NO2
396.98
253.11
Crystal system / Space
group
C2/c
P21/n
P 21/c
Crystal colour
Crystal size (mm)
a (Å)
b (Å)
c (Å)
α (°)
β (°)
Colourless
0.22 x 0.16 x 0.12 0.20 x 0.18 x 0.18
Colourless
Colourless
0.60 x 0.06 x 0.04
13.8876
5.7745
16.4267
90.000
104.791
90.000
1273.67
4
21.8480
12.1257
16.7405
90.000
121.6650
90.000
3774.71
8
20.9365
9.85390
21.4160
90.000
113.2622
90.000
4059.08
8
γ (°)
V (Å3)
Z
Dcalc (g/cm3)
T (K)
1.289
150
0.09
1.300
150
0.13
1.321
150
0.09
μ(Mo Kα) (mm-1)
Reflections collected (Rint) 4264 (0.0289)
9268 (0.025)
2923 (0.030)
Data/Restraints/
Parameters
2471/0/254
9247/60/552
2130/0/173
R1 (I>2σ(I))
R1 / wR2 (all data)
0.059
0.142
0.084
0.123
0.050
0.079

Molecules 2009, 14
4983
3.3. Plant growth inhibition assays
In order to evaluate the growth regulatory potential of the synthesized bis(pyridyl)methanes (4a-h),
two different bioassays were carried out.
3.3.1. Radicle elongation assay on filter paper
The solutions of bis(pyridyl)methanes 4a-h were prepared by dissolving the compounds in
dimethylsufoxide (150 µL). After addition of the surfactant Tween 80 (72 µL), the resulting
suspension was transferred to a volumetric flask and diluted with water to 50 mL, to obtain the final
concentration 5 × 10-4 mol L-1. These suspensions were sonicated for 5 min, resulting in complete
dissolution of the test compound. Then 4 mL aliquots were used to imbibe two sheets of filter paper
(Whatman nº 1) placed in 100 × 15 mm glass Petri dishes. To each dish were added 20 seeds of
Sorghum bicolor L. Moench (Geneze Company, Paracatu, Minas Gerais State, Brazil) or Cucumis
sativus L. (purchased from a local market). The plates were incubated at 25 ºC under fluorescent light
(8 × 40 W) for 72 h. Radicle length was then measured, and total germination recorded. Seeds were
considered to have germinated if a radicle protruded at least 1 mm. Treatments were carried out in a
completely randomized design with five replications. The data, expressed as percentage of radical
growth inhibition with respect to untreated controls, were analyzed using Tukey’s test at 0.05
probability level.
3.3.2. Greenhouse trials
Plastic pots (0.13 L) were filled with acid-washed sand, which was saturated with the solution of
the test compound (60 mL/450 g of sand, corresponding to 6.7 × 10-8 mol a.i./g substrate). Four seeds
of Ipomoea grandifolia, C. sativus or S. bicolor were placed in each pot. Seedlings were grown in a
greenhouse, and watered as required with tap water or, twice a week, with half-strength Hoagland
solution, to maintain the humidity at 13.3% w/w. Twenty-one days after sowing, plants were
harvested, and the roots and aerial parts were separated and weighted. Tissues were then dried at 60 °C
until there was no further weight loss, and the corresponding dry mass was determined. The percentage
of root and aerial part growth inhibition was calculated in relation to the mass of the respective control.
Data were expressed and analyzed as previously described [31].
4. Conclusions
We have described the preparation of a variety of functionalized bis(pyridyl)methanes, starting
from dehydroacetic acid. We have demonstrated that the condensation of 4-hydroxypyridone 3 with
cinnamaldehydes results in the formation of pyran derivatives. In addition, we have described the
effect of the pyridone derivatives prepared on the aerial parts and on the radicle growth of the
dicotyledonous species Ipomoea grandifolia and Cucumis sativus and the monocotyledonous species
Sorghum bicolor. In general the compounds showed some phytotoxic selectivity, being more active
against the dicotyledonous species. It was observed that all tested compounds inhibited the
development of the roots of C. sativus by more than 50% and three of them inhibit the development of
the aerial parts of I. grandifolia by more than 50%. Although no concrete structure-activity

Molecules 2009, 14
4984
relationship analysis could be derived due to the limited number of compounds prepared, the
phytotoxicity reported for this class of bis(pyridyl)methane substances is an indication of the potential
of these substances as lead structures for the development of new compounds with increased activity.
Work is currently underway to achieve this goal.
Acknowledgements
We are grateful to the following Brazilian agencies: CNPq (Conselho Nacional de
Desenvolvimento Científico e Tecnológico) for financial support and research fellowships (AJD,
LCAB) and FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais) for financial
support and a student fellowship (VMMV). We also thank the Indian trusts: Narotam Sekhsaria
Foundation and J. N. Tata Endowment for financial support (AHR).
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Sample Availability: Samples of the compounds 4a, 4b, 4c, 4e, 4f, 4g’ and 4h’ are available from the
authors.
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.
This article is an open-access article distributed under the terms and conditions of the Creative
Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).