Carbohydrate Research p. 475 - 481 (1997)
Update date:2022-08-03
Topics: Mg2+ Continuous enzymatic production GDP-mannose Recombinant GDP-mannose pyrophosphorylase
Fey, Sven
Elling, Lothar
Kragl, Udo
The aim of the presented work is the chemoenzymatic synthesis of GDP- mannose (GDP-Man). Mannose-1-phosphate (Man-1-P) is synthesised by a very convenient chemical method and is activated with GTP and recombinant GDP- mannose pyrophosphorylase (GDP-Man PP) to give GDP-Man. The productivity of the process is improved using reaction engineering techniques. Detailed kinetic studies, modelling of the reaction and simulations of different reaction systems revealed that besides a strong product inhibition the cofactor Mg2+ is a key switch for effective enzymatic synthesis. Using a two-stage cascade of enzyme membrane reactors, GDP-Man could be produced continuously with a space-time yield of 28 gL-1 d-1 and an enzyme consumption of 0.9 Ug-1, which means a six-fold improvement related to batch synthesis.
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