Carbohydrate Research p. 475 - 481 (1997)
Update date:2022-08-03
Topics: Mg2+
Fey, Sven
Elling, Lothar
Kragl, Udo
The aim of the presented work is the chemoenzymatic synthesis of GDP- mannose (GDP-Man). Mannose-1-phosphate (Man-1-P) is synthesised by a very convenient chemical method and is activated with GTP and recombinant GDP- mannose pyrophosphorylase (GDP-Man PP) to give GDP-Man. The productivity of the process is improved using reaction engineering techniques. Detailed kinetic studies, modelling of the reaction and simulations of different reaction systems revealed that besides a strong product inhibition the cofactor Mg2+ is a key switch for effective enzymatic synthesis. Using a two-stage cascade of enzyme membrane reactors, GDP-Man could be produced continuously with a space-time yield of 28 gL-1 d-1 and an enzyme consumption of 0.9 Ug-1, which means a six-fold improvement related to batch synthesis.
View MoreHangzhou Eastbiopharm Co.,Ltd.
Contact:+86-571-88931780
Address:Hangzhou,China
Zhushan County Tianxin Pharmaceutical & Chemical Co., Ltd.
Contact:0086-719-4224892
Address:Tutang Road, Chengguan Town, Zhushan County, Hubei Province
Global United Biotechnology Inc.
Contact:+86-21-61618568
Address:Room 309, Building 6, NO.135, Jinyu Road, Pudong
Contact:27-792-602929
Address:SIDNEY MUFAMADI STREET 009949 X44 0700 POLOKWANE,LIMPOPO
Contact:0091-265-2313036
Address:311, ATLANTIS HEIGHTS SARABHAI MAIN ROAD,VADIWADI ,VADODARA
Doi:10.1021/ja01252a036
(1943)Doi:10.1016/j.poly.2006.01.007
(2006)Doi:10.1021/jm2001585
(2011)Doi:10.1021/jo060516m
(2006)Doi:10.1021/ja061660s
(2006)Doi:10.1039/c4ce01412j
(2014)