Diastereoselective synthesis of highly constrained spiro-β-lactams by the staudinger reaction using an unsymmetrical bicyclic ketene

Article abstract of DOI:10.1002/ejoc.200700260

A rigid bicyclic ketene was used to generate highly constrained polycyclic spiro-β-lactams through the Staudinger reaction. Depending on the imine component, high diastereoselectivity was observed in the process, leading to mainly the cis diastereoisomer

Full text of DOI:10.1002/ejoc.200700260

FULL PAPER
DOI: 10.1002/ejoc.200700260
Diastereoselective Synthesis of Highly Constrained Spiro-β-Lactams by the
Staudinger Reaction Using an Unsymmetrical Bicyclic Ketene
Andrea Trabocchi,[a] Claudia Lalli,[a] Francesco Guarna,[a] and Antonio Guarna*[a]
Keywords: Peptidomimetics / Spiro compounds / Lactams / Cycloaddition / Scaffolds
A rigid bicyclic ketene was used to generate highly con-
strained polycyclic spiro-β-lactams through the Staudinger
reaction. Depending on the imine component, high dia-
stereoselectivity was observed in the process, leading to
mainly the cis diastereoisomer in the case of aromatic imines.
This results from an anti addition of the imine to the ketene,
followed by a conrotatory ring closure in which the hetero-
atom at the 6-position of the scaffold rotates outward because
of torquoelectronic effects.
(© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim,
Germany, 2007)
β-lactams have been described in the literature,[10] and in
recent years many researchers have accomplished the syn-
thesis of spiro-β-lactams through cycloaddition reactions
employing different ketenes and imines.[11]
Introduction
Peptidomimetics are important compounds in the fields
of molecular diversity and drug discovery. For use in drug
discovery, these nonpeptide molecules must mimic the cru-
cial structural properties of bioactive peptides and maintain
some or all of their characteristic bioactivities.[1] Control of
the topological arrangement of the residues that constitute
the pharmacophore in peptidomimetics is accomplished by
the use of molecular scaffolds.[2] Organic chemists use
modern synthetic techniques to make structurally diverse
scaffolds for use as peptidomimetic templates, including cy-
clic, polycyclic and spiro compounds varying in ring size
and in their level of functionalization. In particular, lactam
or bicyclic lactam formation is commonly used for con-
straining the torsion angles for the synthesis of peptidomi-
metics,[3] and β-lactams have been extensively used as syn-
thetic intermediates in organic synthesis (the β-lactam syn-
thon method),[4] thus providing a very useful route to a
number of α- and β-amino acid derivatives and peptides.
The application of spiro-β-lactams in peptidomimetic
chemistry is well-documented, and relevant examples in-
clude the development of constrained β-turn mimetics.[5]
Also, spiro-β-lactams have received attention in medicinal
chemistry owing to their antiviral and antibacterial proper-
ties,[6] as well as recognized activity as cholesterol absorp-
tion inhibitors.[7]
In recent years, our interest in the development of hetero-
cycles and constrained amino acids for peptidomimetic
chemistry has focused on the synthesis of bicyclic 3-aza-
6,8-dioxabicyclo[3.2.1]octane scaffolds as γ/δ-amino acids.
These BTAa (bicycles from tartaric and amino acid deriva-
tives), obtained from the condensation of tartaric acid or
sugar derivatives and amino carbonyl derivatives, proved to
be valuable dipeptide isosteres when inserted into peptide
chains.[12] Accordingly, we reported the synthesis of α-,[13]
β-[14] and γ/δ-amino acids,[12,14,15] as well as tricyclic scaf-
folds containing the 4-hydroxyproline nucleus[16] and
[4.2.1]- and [5.2.1]-sized heterocyclic analogues.[17] We were
interested in further exploring the skeletal diversity of such
constrained scaffolds by generating polycyclic spiro com-
pounds through the Staudinger reaction with different
types of imines. Our aim was to generate compounds pos-
sessing diverse scaffolds to which different functional
groups could be fixed in a stereodefined 3D topological ar-
rangement.
Results and Discussion
Among the strategies developed for the construction of
β-lactams,[8] the reaction of acyl chlorides with imines
(known as the Staudinger reaction[9]) constitutes one of the
most popular procedures. Also, several syntheses of spiro-
Derivatives 2 and 3, obtained from bicyclic Bn-BTG(O)-
OMe (1) (BTG: Bicycles from Tartaric acid and Glycin
derivatives), were used to assess the yield and selectivity re-
sulting from the reaction of the corresponding BTAa-de-
rived ketene with selected imines (Table 1). Ketenes are
commonly generated from acyl chlorides, and the use of
Mukaiyama’s salt for in situ generation of the reactive spe-
cies, even in solid-phase chemistry, has also been re-
ported.[18,19] Both methodologies were thus explored to
[a] Dipartimento di Chimica Organica “Ugo Schiff”, Laboratorio
di Progettazione, Sintesi e Studio di Eterocicli Bioattivi (Hetero-
BioLab), Università degli Studi di Firenze
Via della Lastruccia 13, 50019 Sesto Fiorentino, Italy
Fax: +39-055-4573569
E-mail: antonio.guarna@unifi.it
4594
© 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
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Diastereoselective Synthesis of Highly Constrained Spiro-β-Lactams
Table 1. Selected examples of spiro-β-lactams 4 with general formulas as in Scheme 2.[a]
Entry
Imine
RЈ
R
Product
Yield [%]
cis/trans
1
2
3
4
5
6
7
8
I
Ph
Ph
Ph
Ph
CH(CH2Ph)COOMe
CH2Ph
p-CH3Ph
p-NO2Ph
p-OMePh
p-CH3Ph
6
33
66
14
–
39
24
59
33
1.4:1
1:1.2
15:1
–
10:1
20:1
Ͼ50:1
7:1
II
7
III
IV
V
VI
VII
VIII
8
9
10
11
12
13
Ph
p-NO2Ph
p-OMePh
p-BrPh
p-CH3Ph
p-CH3Ph
[a] Reactions were all conducted in toluene at 110 °C by using method 1.
verify the reactivity of the unsymmetrical bicyclic ketene at not lead to the preferential formation of the corresponding
the 7-position of the scaffold with respect to the imine at spiro-β-lactam after conrotatory ring closure, because hy-
different temperatures and with different solvent systems drolysis of the intermediate N-acyl iminium species was
(Scheme 1).
favoured under all the conditions tested, leading to amide
byproducts 5.
Therefore, more reactive acyl chloride derivative 3 was
used to attain title spiro-β-lactams 4. The addition of pre-
formed acyl chloride 3 to a refluxing CH2Cl2 solution of
the imine and triethylamine (TEA) resulted in poor conver-
sion to spiro-β-lactam 4 after a reaction time of 18 h. How-
ever, spiro-β-lactam 4 was obtained after a reaction time of
24 h by adding a solution of acyl chloride 3 in toluene to a
refluxing toluene solution of the imine and TEA
(method 1). Also, when the reaction was conducted at lower
temperatures, the reverse approach was used, which con-
sisted of the preformation of the ketene by allowing the acyl
chloride to react with TEA, followed by the addition of the
with potassium trimethylsilanolate imine species (method 2). The reaction was explored with
Scheme 1.
Hydrolysis of
1
(TMSOK) in anhydrous THF furnished corresponding car- imines derived from α-amino esters, benzylamine and aro-
boxylic acid 2 in quantitative yield (Scheme 1). Activation matic amines and gave different results in terms of yield
of the carboxylic function of the BTG scaffold by means of and diastereoselectivity, as shown in Table 1.
Mukaiyama’s salt, followed by reaction of either imine I
The nucleophilicity of the amine derivatives comprising
or VII (see Table 1) in anhydrous CH2Cl2, resulted in the the imine proved to influence the yield, as the conversion
formation of corresponding amides 5. This was a conse- proceeded in the order benzyl Ͼ amino ester ≈ aryl. In par-
quence of the hydrolysis of the iminium salt that resulted ticular, substitution on the aniline ring influenced the reac-
from the condensation of the activated acid with the corre- tivity, as p-NO2-aryl-N-substituted imine IV (Table 1, En-
sponding imine (Scheme 2). Replacement of CH2Cl2 with try 4) failed to react with the bicyclic ketene, probably as a
toluene as the solvent and a change in the order of addition result of the unfavourable electronic effects in the
of the reagents to the reaction mixture resulted in the same Staudinger reaction, whereas p-OMe-aryl-N-substitution
amide byproducts. The effect of temperature was also inves- (Table 1, Entry 5) gave the highest yield of the benzalde-
tigated, but no improvement in the reactivity was obtained hyde-derived aryl imines (Table 1, Entries 3–5). Also, the
in refluxing chloroform or toluene solutions of the reactive nature of the aromatic aldehyde-derived moiety agreed with
Mukaiyama’s adduct with imine I (Table 1). Thus, the use the hypothesis that an electron-rich imine could improve
of Mukaiyama’s salt with bicyclic carboxylic acid 2 could the yield of the corresponding spiro-β-lactam, as p-NO2-
Scheme 2.
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A. Trabocchi, C. Lalli, F. Guarna, A. Guarna
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aryl-C-substitution gave 11 in 24% yield, whereas p-OMe-
aryl-C-substitution yielded corresponding spiro compound
12 in 54% yield (Table 1, Entries 6 and 7, respectively). In
the latter case (Table 1, Entry 7), the order of addition of
the reagents proved to influence the ratio of 4/5 as a conse-
quence of the hydrolysis of the intermediate iminium spe-
cies. Specifically, method 1 proved to give the spiro-β-lac-
tam even at 60 °C, whereas method 2, consisting of the pre-
formation of the ketene followed by imine addition, resulted
in a 1:1 mixture of spiro-β-lactam 4/amide byproduct 5. In
terms of stereoselectivity, imine I (derived from benzalde-
hyde and phenylalanine methyl ester; Table 1, Entry 1) gave
a mixture of two compounds as a result of poor diastereo-
selectivity, as did imine II (derived from benzylamine;
Table 1, Entry 2) However, aromatic imines III–VIII gave in
all cases a major stereoisomer.
Figure 1. 1H NMR signal of 5-H of compound 7 resulting from
the reaction of 3 with imine II. The reactions were carried out at
110 °C (top left), 80 °C (top right) and 20 °C (bottom left).
Spiro-β-lactam formation by using benzylamine-derived
imine II (Table 1, Entry 2) was further investigated as a
function of the reaction temperature. In this case, method 2,
involving preformation of the ketene, was found to be cru-
cial for achieving corresponding spiro compound 7. Inter-
estingly, we observed an effect of the temperature on the
diastereomeric ratio, as shown in Table 2. Specifically, when
moving from 110 to 0 °C we observed a slight increase in
the stereoselectivity and a shift towards the preferential for-
mation of one of the two diastereoisomers (Figure 1). The
structure of the two stereoisomers was assigned by NOESY
experiments of the mixture resulting from the reaction at
20 °C (Table 2, Entry 2). In the major stereoisomer, a strong
NOE signal was observed between 1-H of the bicyclic com-
pound and 4Ј-H of the azetidine ring, which suggests that
spiro-β-lactam 7 with the general structure of cis-4 as in
Scheme 2 was obtained as the major compound at tempera-
tures below 60 °C.
Figure 2. X-ray structure of compound 12.
Table 2. Effect of the temperature on the diastereomeric ratio of
spiro-β-lactam 7 as in Table 1, Entry 2.[a]
Figure 3. Selected NOE contacts for compound 12.
Entry
Temperature [°C]
Yield [%]
cis/trans
(Scheme 2) would thus have resulted from initial attack of
the imine species on the bicyclic ketene in the anti orienta-
tion with respect to the O-6 oxygen atom of the scaffold,
followed by a preferential outward conrotatory ring closure
of the O-6 substituent favoured by a torquoelectronic effect
(Figure 4).
1
2
3
4
5
0
22
14
24
19
66
2.7:1
2.2:1
1:1
1.1:1
1:1.2
20
60
80
110
[a] Reactions were all conducted by using method 2.
X-ray and NMR spectroscopic analysis of compound 12
revealed information about the structure of the major dia-
stereoisomer. NOESY experiments agreed with the X-ray
crystal structure of 12 as shown in Figure 2,[20] as demon-
strated by a NOE crosspeak between 4Ј-H of the azetidine
ring and 1-H of the bicyclic scaffold, and also by a weak
NOE crosspeak between the CH2 protons at N-3 and the
4Ј-p-OMePh hydrogen atoms (Figure 3).
The mechanism of the reaction is widely thought to in-
volve nucleophilic attack of the imine on the ketene species
to give a zwitterionic intermediate, which undergoes a sub-
sequent conrotatory ring closure to generate the spiro-β-
lactam species. In agreement with similar substrates
Figure 4. Proposed mechanism for the stepwise Staudinger reac-
reported in the literature,[11f] diastereoisomer cis-12 tion.
4596
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Eur. J. Org. Chem. 2007, 4594–4599

Diastereoselective Synthesis of Highly Constrained Spiro-β-Lactams
1 equiv.) and dry TEA (1.5 equiv.) in dry toluene (0.4 ) was stirred
at the desired temperature under a nitrogen atmosphere for 10 min,
and a solution of imine II (1.1 equiv.) in dry toluene (0.2 ) was
then added. The mixture was stirred at the desired temperature
under a nitrogen atmosphere for 15 h, and it was then washed with
saturated aqueous NaHCO3 and brine. The organic layer was dried
with Na2SO4, filtered and concentrated in vacuo to give a dark
oil, which was purified by flash chromatography (petroleum ether/
EtOAc, 1:1).
This effect has been demonstrated to be very pronounced
for ketenes bearing a heteroatom adjacent to the carbon–
carbon double bond,[11f] thus agreeing with the high stereo-
selectivity observed in our compounds derived from aro-
matic imines. The minor stereoisomer could result from syn
attack of the imine species to give the trans compound as
shown in Figure 4. This hypothesis is supported by the ab-
sence of NOESY peaks either between 4Ј-H of the azetidine
ring and 1-H of the bicyclic scaffold, thus excluding the
other possible cis stereoisomer, or between the CH2 protons
at N-3 and the 4Ј-aryl hydrogen atoms, thus excluding the
other possible trans structure. The loss of stereoselectivity
in the case of benzyl- and phenylalanine-derived imines may
take account of the higher conformational freedom due to
the lack of conjugation within the imine with respect to the
aromatic ones.
(1R,5S,7R)-3-Benzyl-2-oxo-6,8-dioxa-3-azabicyclo[3.2.1]octane-7-
carbonyl Chloride (3): Bn-BTG(O)-OMe (1) was dissolved in anhy-
drous THF to give a 0.1  solution, and TMSOK (1.5 equiv.) was
added in one portion. After stirring 1.5 h at room temperature, the
mixture was diluted with EtOAc, washed with 5% KHSO4 and
brine and dried with Na2SO4. Evaporation of the organic phase
1
gave pure acid 2 in 95% yield. H NMR (200 MHz, CDCl3): δ =
7.36–7.18 (m, 5 H, Ph), 5.92 (d, 3JH,H = 2.2 Hz, 1 H, 5-H), 5.12 (s,
1 H, 1-H), 4.96 (s, 1 H, 7-H), 4.56 (s, 2 H, CH2-Ph), 3.39 (dd, 2JH,H
3
2
= 12.4 Hz, JH,H = 2.4 Hz, 1 H, 4-H), 3.14 (d, JH,H = 12.0 Hz, 1
H, 4-H) ppm. To a solution of acid 2 (1 equiv.) in dry CH2Cl2
(0.7 ) was slowly added a solution of oxalyl chloride (5 , 3 equiv.)
in dry CH2Cl2, and a drop of dry DMF was then added. The mix-
ture was stirred at room temp. under a nitrogen atmosphere for
15 h, and the solvent was then concentrated in vacuo to give the
corresponding acyl chloride, which was immediately used in the
Staudinger reaction.
Conclusions
The synthesis of highly constrained spiro-β-lactams from
a bicyclic ketene was achieved by the Staudinger reaction.
The outcome of the reaction indicated that the aromatic
imines are best choice for the generation of a new molecular
architecture bearing aromatic functional groups, as in this
case the reaction proceeded with high stereoselectivity to
produce the corresponding cis spiro-β-lactams as the major
compounds. Both aliphatic- and amino acid derived imines
provided mixtures of cis- and trans spiro-β-lactams in vari-
able amounts. Given the potential structural diversity of the
ketene bicyclic scaffold,[12a] this method is of interest for
the generation of densely functionalized molecular scaffolds
having restricted conformational freedom and a stereo-
defined 3D topological array of the substituents. Optimized
reaction conditions may be applied for the subsequent gen-
eration of polycyclic spiro-β-lactams as peptidomimetics for
biomedical research.
Spiro Compound 6: White solid, 150 mg, 33% yield. 1H NMR
(400 MHz, CDCl3) 3:2 mixture of diastereomers A and B: δ = 7.40–
3
7.19 (m, 15 H, Ph, A+B), 5.59 (d, JH,H = 2.4 Hz, 1 H, 5-H, A),
3
5.32 (d, JH,H = 2.4 Hz, 1 H, 5-H, B), 4.94 (s, 1 H, 1-H, A), 4.89
2
(d, JH,H = 15.1 Hz, CH2, A), 4.88 (s, 2 H, 4Ј-H, A+B), 4.82 (s, 1
2
H, 1-H, B), 4.74 (d, JH,H = 15.3 Hz, 1 H, CH2, B), 4.52 (m, 1 H,
2
α-H, B), 4.50 (d, JH,H = 15.3 Hz, 1 H, CH2, B), 4.05 (m, 1 H, α-
2
H, A), 3.91 (d, JH,H = 14.7 Hz, 1 H, CH2, A), 3.79 (s, 3 H, OMe,
2
B), 3.71 (s, 3 H, OMe, A), 3.56 (dd, JH,H = 14.0, 3JH,H = 11.9 Hz,
2
3
1 H, β-H, A), 3.30 (dd, JH,H = 14.0 Hz, JH,H = 4.6 Hz, 1 H, β-H
2
A), 3.18 (m, 2 H, 4-H, B), 3.15 (m, 1 H, β-H, B), 3.13 (dd, JH,H
= 12.4 Hz, 3JH,H = 3.0 Hz, 1 H, 4-H, B), 3.05 (dd, 2JH,H = 12.7 Hz,
3JH,H = 2.4 Hz, 1 H, 4-H, A), 3.00 (dd, JH,H = 14.4 Hz, JH,H
=
2
3
2
9.6 Hz, 1 H, β-H, B), 2.45 (d, JH,H = 14.0 Hz, 1 H, 4-H, A) ppm.
13C NMR (50 MHz, CDCl3) mixture of diastereomers A and B: δ
= 169.2 (s, A), 168.2 (s, B), 164.5 (s, A), 163.3 (s, B), 136.8 (s, A),
136.6 (s, B), 135.3 (s, A), 134.7 (s, B), 134.0 (s, A), 132.7 (s, B),
129.3 (d), 129.2 (d), 128.9 (d), 128.8 (d), 128.7 (d), 128.5 (d), 128.3
(d), 128.2 (d), 127.9 (d), 127.7 (d), 127.6 (d), 127.2 (d), 127.1 (d),
100.5 (d, B), 100.1 (d, A), 92.5 (s, A), 91.9 (s, B), 79.6 (d, A), 76.8
(d, B), 70.9 (d, A), 62.9 (d, B) 58.9 (d), 57.9 (d), 57.1 (d), 52.9 (q),
50.7 (d, A), 50.6 (d, B), 48.6 (d, A), 48.4 (d, B), 36.2 (t, B), 35.6
(d, A) ppm. MS: m/z (%) = 512 (3) [M]+, 307 (48), 268 (11), 160
Experimental Section
General Remarks: Melting points are uncorrected. Chromato-
graphic separations were performed on silica gel by using flash-
column techniques. Rf values refer to TLC carried out on 25 mm
silica gel plates (Merck F254) with the same eluent indicated as for
column chromatography. EI mass spectra were carried out at 70 eV
ionizing voltage.
(76), 148 (21), 117 (13), 91 (100). IR (CDCl ): ν = 3072, 2931, 1792,
˜
3
General Procedure for the Synthesis of Spiro Compounds 6–13
(Method 1): A solution of the proper imine (1.1 equiv.) and dry
TEA (1.5 equiv.) in dry toluene (0.2 ) was heated to reflux under
a nitrogen atmosphere; a mixture of Bn-BTG(O)-Cl (3; 250 mg,
0.89 mmol, 1 equiv.) in dry toluene (0.4 ) was then added. The
mixture was stirred at 110 °C under a nitrogen atmosphere for 16 h,
and it was then washed with saturated aqueous NaHCO3 and brine.
The organic layer was dried with Na2SO4, filtered and concentrated
in vacuo to give a dark oil. The pure products were afforded after
purification by flash chromatography (petroleum ether/EtOAc,
1:1).
1743, 1664, 1493, 1458 cm–1.
Spiro Compound 7: White solid, 258 mg, 66% yield. 1H NMR
(400 MHz, CDCl3) mixture of diastereomers A and B: δ = 7.48–
3
7.07 (m, 15 H, Ph, A+B), 5.68 (d, JH,H = 2.6 Hz, 1 H, 5-H, B),
3
5.42 (d, JH,H = 2.3 Hz, 1 H, 5-H, A), 4.98 (s, 1 H, 4Ј-H, B), 4.97
2
(d, JH,H = 14.2 Hz, 1 H, CH2, A), 4.96 (s, 1 H, 4Ј-H, A), 4.84 (d,
2JH,H = 15.2 Hz, 1 H, CH2, A), 4.83 (d, 2JH,H = 14.7 Hz, 1 H, CH2,
2
B), 4.82 (s, 1 H, 1-H, B), 4.78 (d, JH,H = 14.8 Hz, 1 H, CH2, B),
2
4.55 (s, 1 H, 1-H, A), 4.45 (d, JH,H = 14.8 Hz, 1 H, CH2, A), 3.99
2
2
(d, JH,H = 14.8 Hz, 1 H, CH2, B), 3.97 (d, JH,H = 14.7 Hz, 1 H,
2
2
General Procedure for the Synthesis of Spiro Compound
7
CH2, B), 3.94 (d, JH,H = 14.7 Hz, 1 H, CH2, A), 3.22 (dd, JH,H
(Method 2): A solution of Bn-BTG(O)-Cl (3; 250 mg, 0.89 mmol,
= 12.5 Hz, 3JH,H = 2.4 Hz, 1 H, 4-H, A), 3.18 (dd, 2JH,H = 12.5 Hz,
Eur. J. Org. Chem. 2007, 4594–4599
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A. Trabocchi, C. Lalli, F. Guarna, A. Guarna
FULL PAPER
3JH,H = 2.7 Hz, 1 H, 4-H, B), 3.10 (dd, JH,H = 12.5 Hz, JH,H
2
3
=
CDCl3): δ = 7.34–7.02 (m, 11 H, Ph), 6.85–6.80 (m, 2 H, Ph), 5.72
(d, JH,H = 2.6 Hz, 1 H, 5-H), 5.34 (s, 1 H, PhCHN), 5.07 (s, 1 H,
2
3
2.3 Hz, 1 H, 4-H, A), 2.54 (d, JH,H = 12.3 Hz, 1 H, 4-H, B) ppm.
2
2
13C NMR (50 MHz, CDCl3) mixture of diastereomers A and B: δ 1-H), 4.98 (d, JH,H = 14.6 Hz, 1 H, CH2Ph), 4.05 (d, JH,H
=
=
2
= 166.8 (s, B), 164.6 (s, B), 164.5 (s, A), 163.6 (s, A), 135.3 (s, A),
134.7 (s, B), 134.4 (s, B), 134.3 (s, A), 133.8 (s, B), 132.8 (s, A),
128.9 (d), 128.8 (d), 128.7 (d), 128.6 (d), 128.5 (d), 128.4 (d), 128.3
14.6 Hz, 1 H, CH2Ph), 3.79 (s, 3 H, OCH3), 3.26 (dd, JH,H
3
2
12.4 Hz, JH,H = 2.6 Hz, 1 H, 4-H), 2.68 (d, JH,H = 12.4 Hz, 1 H,
4-H), 2.26 (s, 3 H, CH3) ppm. 13C NMR (50 MHz, CDCl3): δ =
(d), 128.2 (d), 128.0 (d), 127.8 (d), 127.7 (d), 127.6 (d), 100.6 (d, 164.6 (s, C=O), 163.9 (s, C=O), 159.8 (s, Ph), 134.8 (s, Ph), 134.3
B), 100.1 (d, A), 93.2 (s, B), 92.6 (s, A), 79.5 (d, A), 76.7 (d, B),
68.1 (d, B), 62.3 (d, A), 50.8 (t, A), 50.5 (t, B), 48.6 (t, A), 48.4 (t,
B), 44.6 (t, 2 C) ppm. MS: m/z (%) = 440 (7) [M]+, 307 (63), 292
(17), 216 (15), 160 (75), 148 (27), 132 (22), 91 (100), 65 (25). IR
(s, Ph), 134.1 (s, Ph), 129.6 (d, Ph), 128.9 (d, Ph), 128.6 (d, Ph),
128.0 (d, Ph), 124.7 (s, Ph), 117.7 (d, Ph), 114.1 (d, Ph), 100.2 (d,
C-5), 92.2 (s, C-7), 76.9 (d, PhCHN), 62.2 (d, C-1), 55.3 (q, OCH3),
50.9 (t, C-4), 48.9 (t, CH2Ph), 21.2 (q, CH3) ppm. MS: m/z (%) =
470 (7) [M]+, 337 (74), 225 (54), 190 (73), 161 (22), 91 (100), 65
(CDCl ): ν = 3055, 2923, 1767, 1676, 1496, 1455 cm–1.
˜
3
(24). IR (CDCl3):
ν =
˜
3063, 2926, 1759, 1671, 1515 cm–1.
Spiro Compound 8: Yellow solid, 55 mg, 14% yield. M.p. 86–89 °C.
C28H26N2O5 (470.5): calcd. C 71.47, H 5.57, N 5.95; found C 71.47,
H 5.55, N 5.96.
1
[α]2D5 = –84.44 (c = 0.7, CH2Cl2). H NMR (400 MHz, CDCl3): δ
= 7.33–7.03 (m, 14 H, Ph), 5.70 (d, 3JH,H = 2.6 Hz, 1 H, 5-H), 5.40
2
(s, 1 H, PhCHN), 5.09 (s, 1 H, 1-H), 5.04 (d, JH,H = 14.6 Hz, 1
Spiro Compound 13: Yellow solid, 152 mg, 33% yield. M.p. 96–
99 °C. [α]2D5 = –123.02 (c = 0.95, CH2Cl2). 1H NMR (200 MHz,
CDCl3): δ = 7.42–7.16 (m, 9 H, Ph), 7.05–6.99 (m, 4 H, Ph), 5.68
H, CH2Ph), 4.03 (d, 2JH,H = 14.3 Hz, 1 H, CH2Ph), 3.41 (dd, 2JH,H
3
2
= 12.4 Hz, JH,H = 2.6 Hz, 1 H, 4-H), 2.64 (d, JH,H = 12.4 Hz, 1
H, 4-H), 2.23 (s, 3 H, CH3) ppm. 13C NMR (CDCl3): δ = 164.6 (s,
C=O), 163.7 (s, C=O), 134.6 (s, Ph), 134.5 (s, Ph), 133.9 (s, Ph),
132.9 (s, Ph), 129.5 (d, 2 C, Ph), 128.9 (d, 2 C, Ph), 128.8 (d, Ph),
128.6 (d, 2 C, Ph), 128.5 (d, 2 C, Ph), 128.1 (d, Ph), 127.2 (d, 2 C,
Ph), 117.7 (d, Ph), 100.1 (d, C-5), 92.2 (s, C-7), 76.9 (d, PhCHN),
62.5 (d, C-1), 50.9 (t, C-4), 48.9 (t, CH2Ph), 21.1 (q, CH3) ppm.
MS: m/z (%) = 440 (8) [M]+, 307 (19), 195 (41), 160 (51), 91 (100),
3
(d, JH,H = 2.6 Hz, 1 H, 5-H), 5.37 (s, 1 H, PhCHN), 5.09 (s, 1 H,
2
2
1-H), 4.87 (d, JH,H = 14.6 Hz, 1 H, CH2Ph), 4.14 (d, JH,H
=
2
3
13.2 Hz, 1 H, CH2Ph), 3.26 (dd, JH,H = 12.4 Hz, JH,H = 2.6 Hz,
1 H, 4-H), 2.69 (d, JH,H = 12.1 Hz, 1 H, 4-H), 2.25 (s, 3 H, CH3)
2
ppm. 13C NMR (50 MHz, CDCl3): δ = 164.5 (s, C=O), 163.5 (s,
C=O), 134.7 (s, Ph), 134.6 (s, Ph), 133.8 (s, Ph), 132.1 (s, Ph), 131.8
(d, Ph), 129.7 (d, Ph), 129.0 (d, Ph), 128.9 (d, Ph), 128.7 (d, Ph),
128.1 (d, Ph), 122.8 (s, Ph), 117.6 (d, Ph), 100.3 (d, C-5), 92.2 (s,
C-7), 76.9 (d, PhCHN), 61.9 (d, C-1), 50.9 (t, C-4), 48.9 (t, CH2Ph),
21.2 (q, CH3) ppm. MS: m/z (%) = 520 (5) [M + 1]+, 518 (5) [M –
1]+, 387 (13), 385 (13), 275 (22), 273 (22), 240 (15), 238 (15). IR
65 (18). IR (CDCl ): ν = 3067, 2928, 1760, 1673 cm–1. C H N O
˜
3
27 24
2
4
(440.5): calcd. C 73.62, H 5.49, N 6.36; found C 73.59, H 5.48, N
6.34.
Spiro Compound 10: Yellow solid, 158 mg, 39% yield. M.p. 94–
97 °C. [α]2D5 = –124.98 (c = 0.8, CH2Cl2). 1H NMR (400 MHz,
CDCl3): δ = 7.35–7.23 (m, 12 H, Ph), 6.85–6.81 (m, 2 H, Ph), 5.75
(CDCl3):
ν =
˜
3054, 2991, 1762, 1672, 1515, 1488 cm–1.
C27H23BrN2O4 (519.4): calcd. C 62.44, H 4.46, N 5.39; found C
62.41, H 4.44, N 5.39.
3
(d, JH,H = 2.6 Hz, 1 H, 5-H), 5.41 (s, 1 H, PhCHN), 5.12 (s, 1 H,
2
2
1-H), 5.08 (d, JH,H = 14.6 Hz, 1 H, CH2Ph), 4.05 (d, JH,H
=
14.7 Hz, 1 H, CH2Ph), 3.78 (s, 3 H, OCH3), 3.30 (dd, 2JH,H = 12.1,
Acknowledgments
3JH,H = 2.6 Hz, 1 H, 4-H), 2.67 (d, JH,H = 12.1 Hz, 1 H, 4-H)
3
ppm. 13C NMR (50 MHz, CDCl3): δ = 164.7 (s, C=O), 163.4 (s,
C=O), 156.5 (s, Ph), 134.6 (s, Ph), 132.9 (s, Ph), 129.9 (s, Ph), 128.9
(d, Ph), 128.6 (d, Ph), 128.5 (d, Ph), 128.0 (d, Ph), 127.2 (d, Ph),
119.0 (d, Ph), 114.3 (d, Ph), 100.1 (d, C-5), 92.3 (s, C-7), 76.9 (d,
PhCHN), 62.6 (d, C-1), 55.5 (q, OCH3), 50.9 (t, C-4), 48.9 (t,
CH2Ph) ppm. MS: m/z (%) = 456 (25) [M]+, 307 (23), 211 (66), 196
The authors thank Università degli Studi di Firenze and Ministero
dell’Università e della Ricerca (MUR) for financial support.
Maurizio Passaponti and Brunella Innocenti are gratefully ac-
knowledged for their technical support and Dr. Cristina Faggi for
X-ray analysis. Ente Cassa di Risparmio is acknowledged for grant-
ing the 400 MHz NMR instrument.
(33), 160 (75), 148 (18), 91 (100), 77 (17), 65 (13). IR (CDCl ): ν =
˜
3
3081, 2921, 1758, 1672, 1513 cm–1. C27H24N2O5 (456.5): calcd. C
71.04, H 5.30, N 6.14; found C 71.06, H 5.32, N 6.13.
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Spiro Compound 11: Yellow solid, 104 mg, 24% yield. M.p. 97–
100 °C. [α]2D5 = –121.72 (c = 0.95, CH2Cl2). 1H NMR (200 MHz,
CDCl3): δ = 8.08 (d, 3JH,H = 8.4 Hz, 2 H, Ar), 7.42–7.05 (m, 11 H,
3
Ph), 5.74 (d, JH,H = 2.20 Hz, 1 H, 5-H), 5.46 (s, 1 H, PhCHN),
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5.11 (s, 1 H, 1-H), 4.81 (d, JH,H = 14.3 Hz, 1 H, CH2Ph), 4.25 (d,
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2JH,H = 14.3 Hz, 1 H, CH2Ph), 3.35 (dd, JH,H = 12.1, JH,H
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2
2.6 Hz, 1 H, 4-H), 2.74 (d, JH,H = 12.4 Hz, 1 H, 4-H), 2.28 (s, 3
H, CH3) ppm. 13C NMR (50 MHz, CDCl3): δ = 164.5 (s, C=O),
163.8 (s, C=O), 148.0 (s, Ph), 140.5 (s, Ph), 135.0 (s, Ph), 134.5 (s,
Ph), 133.5 (s, Ph), 129.8 (d, Ph), 129.0 (d, Ph), 128.9 (d, Ph), 128.4
(d, Ph), 128.0 (d, Ph), 123.9 (d, Ph), 117.5 (d, Ph), 100.4 (d, C-5),
92.4 (s, C-7), 76.9 (d, PhCHN), 61.5 (d, C-1), 51.0 (t, C-4), 49.2 (t,
CH2Ph), 21.1 (q, CH3) ppm. MS: m/z (%) = 485 (5) [M]+, 352 (13),
261 (23), 240 (30), 148 (32), 91 (100), 65 (15). IR (CDCl ): ν =
˜
3
3053, 2947, 1765, 1673, 1525 cm–1. C27H23N3O6 (485.5): calcd. C
66.80, H 4.78, N 8.66; found C 66.83, H 4.80, N 8.69.
Spiro Compound 12: Yellow solid, 247 mg, 59% yield. M.p. 99–
101 °C. [α]2D5 = –103.63 (c = 0.95, CH2Cl2). 1H NMR (400 MHz,
4598
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Eur. J. Org. Chem. 2007, 4594–4599

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Received: March 23, 2007
Published Online: July 10, 2007
Eur. J. Org. Chem. 2007, 4594–4599
© 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
www.eurjoc.org
4599