SINGLE-STEP SYNTHESIS OF KYOTORPHIN IN FROZEN SOLUTIONS BY CHYMOTRYPSIN

Article abstract of DOI:10.1016/S0957-4166(00)80359-6

A novel method of Kyotorphin (H-Tyr-Arg-OH) synthesis without protecting and deprotecting procedures is described - α-chymotrypsin-catalyzed aminolysis of H-Tyr-OEt by H-Arg-OH in frozen mixtures.In this one step process yields of the peptide product above 80percent have been obtained.