SYNTHESIS OF OLIGODEOXYRIBONUCLEOTIDES CONTAINING A 3'-TERMINAL PHOSPHATE GROUP

Article abstract of DOI:10.1007/BF00580075

Two methods for synthesizing oligodeoxyribonucleotides containing 3'-terminal phosphate groups have been developed.The first consists in the synthesis and the introduction into the oligonucleotides of a block containing a 3'-terminal bis(2-cyanoethyl) phosphate group and the second in the use of the transesterification reaction of oligonucleotides with 2-cyanoethanol in the presence of cesium fluoride.Both methods permit the fairly effective synthesis of oligodeoxyribonucleotide 3'-phosphates.