SEMISYNTHESIS I - CLEAVAGE OF METHIONYL PEPTIDE BONDS BY SULPHENYL CHLORIDES.

Article abstract of DOI:10.1016/S0040-4020(01)96406-3

Methoxycarbonyl sulphenyl chloride (Scm.Cl) and o-nitrophenyl-sulphenyl chloride (Nps.Cl) have been shown to cleave methionyl peptide bonds in a manner similar to that found with cyanogen bromide.These new methods of cleavage of methionyl peptide bonds were illustrated by digestion of H.Gly.Met.Ala.OH trifluoroacetate, the (1-32) fragment of adrenocorticotropic hormone <(1-32) ACTH> and Hen Egg-White Lysozyme.