FULL PAPERS
General Procedure for Enzymatic Reactions
Acknowledgements
The activity of lyophilized HNL powders was determined
This work was financially supported by the National Natural
Science Foundation of China (No. 21536004, 21672063 and
[31]
according to Hanefeldꢀs work. HNL was firstly diluted in
00 mM citrate with an appropriate pH value. Aldehyde
the total volume of aldehyde and aqueous phase makes up
1
(
2
(
1472223), the Shanghai Science and Technology Program
No. 15JC1400403), SKLBE (No. 2060204) and the Funda-
mental Research Funds for the Central Universities (No.
22201514039). We also thanks to Professor Li-Ming Liu
State Key Laboratory of Food Science and Technology, Jian-
half of the system) was added as follows, then the reaction
was started with the addition of 2M HCN-MTBE. The reac-
tion was traced by thin-layer chromatography (TLC) until
no more product was formed. Then the reaction was forced
to terminate by ethyl acetate (EA) extraction, the combined
2
(
gnan University) for kindly providing the strain of P. pastoris
X33, Dr. Wen-Ya Lu (SIOC) for his helpful discussions, and
Mr. Zuming Lin (SIOC) for experimental assistance.
organic phase was washed by aqueous saturated FeCl until
3
the color of FeCl remained unchanged, dried over Na SO ,
3
2
4
and evaporated under reduced pressure, and purified by fast
chromatography on silica gel.
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Racemic Cyanohydrins Standard and Acetylated
Derivative for HPLC Analysis
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1
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Determination of the Biotransformation Conversion
and Enantiometric Excess
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[
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4
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8
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Adv. Synth. Catal. 0000, 000, 0 – 0
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