NADPH regeneration by glucose dehydrogenase from Gluconobacter scleroides for l-leucovorin synthesis.

Article abstract of DOI:10.1271/bbb.56.701

A new process for (6S)-tetrahydrofolate production from dihydrofolate was designed that used dihydrofolate reductase and an NADPH regeneration system. Glucose dehydrogenase from Gluconobacter scleroides KY3613 was used for recycling of the cofactor. The reaction mixture contained 200 mM dihydrofolate, 220 mM glucose, 2 mM NADP, 14.4 U/ml dihydrofolate reductase, and 14.4 U/ml Glucose dehydrogenase, and the reaction was complete after incubation at pH 8.0, and 40 degrees C for 2.5 hr. With (6S)-tetrahydrofolate as the starting material, l-leucovorin was synthesized via a methenyl derivative. The purity of the l-leucovorin was 100%, and its diastereomeric purity was greater than 99.5% d.e. as the (6S)-form.

Full text of DOI:10.1271/bbb.56.701

Bioscience, Biotechnology, and Biochemistry
ISSN: 0916-8451 (Print) 1347-6947 (Online) Journal homepage: http://www.tandfonline.com/loi/tbbb20
NADPH Regeneration by Glucose Dehydrogenase
from Gluconobacter scleroides for l-Leucovorin
Synthesis
Tamotsu Eguchi, Yukihiro Kuge, Kunimi Inoue, Naohiro Yoshikawa, Kenichi
Mochida & Takayuki Uwajima
To cite this article: Tamotsu Eguchi, Yukihiro Kuge, Kunimi Inoue, Naohiro Yoshikawa, Kenichi
Mochida & Takayuki Uwajima (1992) NADPH Regeneration by Glucose Dehydrogenase
from Gluconobacter scleroides for l-Leucovorin Synthesis, Bioscience, Biotechnology, and
Biochemistry, 56:5, 701-703, DOI: 10.1271/bbb.56.701
To link to this article: http://dx.doi.org/10.1271/bbb.56.701
Published online: 12 Jun 2014.
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