ENZYMATIC FORMATION OF PROTOPINES BY A MICROSOMAL CYTOCHROME P-450 SYSTEM OF CORYDALIS VAGINANS

Article abstract of DOI:10.1016/S0040-4039(00)96715-7

A microsomal cytochrome P-450-NADPH dependent enzyme which hydroxylates stereo- and regiospecifically carbon atom 14 of (S)-cis-N-methyltetrahydroprotoberberines has been discovered in a number of plant cell cultures originating from species containing protopine alkaloids; the monooxygenase was solubilized, partially purified (100-fold) and characterized.