Science China Chemistry p. 1702 - 1710 (2011)
Update date:2022-08-31
Topics:
Chen, Miaojuan
Qu, Dezhong
Chi, Weilin
Wang, Wei
Ren, Xiaoshuai
Cong, Shujie
Liang, Peizhou
Feng, Shipeng
Zhang, Biliang
Labelling and identification of proliferating cells is important for the study of physiological or pathological processes in high-content screening (HCS) assays. Here we describe ethynyl deoxyuridine (EdU) as a biomarker for the assessment of cell proliferation and clearly demonstrate the feasibility of the EdU-labelling method for use in HCS assays. EdU detection is highly robust, reproducible, technically simple, and well suited for automated segmentation, which provides an excellent alternative for setting up multiplexed HCS assays of siRNA, miRNA and small-molecule libraries.
View MoreXinchang Jiu Xin Pharmaceutical Co., Ltd
website:http://www.jiuxinpharm.com
Contact:86 137 5756 8585
Address:Poyang industry Park
Nantong Baokai Chemical Co., Ltd (Hangzhou Baokai Bio-Chemicals Co.,Ltd.)
Contact:+86-513-83886111
Address:No. 68 Suzhou road ,Binjiang Fine Chemical Industrial Park , Beixin town, Qidong city ,Jiangsu province
Jiangsu Jiuri Chemical Co.,Ltd.
Contact:+86-519-82118868
Address:Tianwang Town, Jurong City, Jiangsu Province, China
Shantou Baokang Pharmaceutical Co., Ltd.
Contact:+86-754-88873487
Address:5/F B Block Huangshan Bldg Huangshan Rd Shantou
TANGSHAN SANJIANG CHEMICAL CO.,LTD
Contact:86-315-2239987
Address:NANPU ECNOMIC DEVELOPMENT ZONE,TANGSHAN ,CHINA
Doi:10.1002/ejoc.200601100
(2007)Doi:10.1080/00397919408020768
(1994)Doi:10.1039/c6ra19840f
(2016)Doi:10.1055/s-0031-1291141
(2012)Doi:10.1021/jo00172a044
(1983)Doi:10.1021/acs.jmedchem.0c02077
(2021)