Exchange of the valine 2-H in the biosynthesis of L-δ-(α-aminoadipoyl)-L-cysteinyl-D-valine

Article abstract of DOI:10.1016/S0040-4020(01)89904-X

Incubation of [2-²H]-valine with purified ACV synthetase from both Cephalosporium acremonium and Streptomyces davuligerus produced L-δ-(α-aminoadipoyl)-L-cysteinyl-D-valine (ACV), determined by the essentially complete (>95%) loss of deuterium from the α position of the incorporated valine. Incubations with deuterium oxide/water as solvent produced ACV with significant incorporation of deuterium into the valinyl residue. These observations confirm the prior proposal that a single multifunctional enzyme is responsible for both the formation of the peptide bonds of ACV and the epimerisation of the valinyl residue.