Phosphorylation of ganciclovir phosphonate by cellular GMP kinase determines the stereoselectivity of anti-human cytomegalovirus activity

Article abstract of DOI:10.1080/15257770008033013

A racemic mixture of ganciclovir phosphonate was resolved by stereoselective phosphorylation using GMP kinase. The R-enantiomer of ganciclovir phosphonate was active against human cytomegalovirus but the S- enantiomer was less active. We show that enantiomeric selectivity of antiviral activity for ganciclovir phosphonate was conferred by stereoselective phosphorylations by mammalian enzymes, not by stereoslective inhibition of DNA polymerase from human cytomegalovirus.

Full text of DOI:10.1080/15257770008033013

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Nucleosides, Nucleotides and Nucleic
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Phosphorylation of Ganciclovir
Phosphonate by Cellular GMP Kinase
Determines the Stereoselectivity of Anti-
Human Cytomegalovirus Activity
Wayne H. Miller a , Lilia M. Beauchamp a , Eric Meade a , John E.
Reardon a , Karen K. Biron a , Albert A. Smith a , Charles A. Goss a
Richard L. Miller a
&
a Glaxo Wellcome Inc. , PO Box 13398, Research Triangle Park, NC,
27709-3398
Published online: 24 Sep 2006.
To cite this article: Wayne H. Miller , Lilia M. Beauchamp , Eric Meade , John E. Reardon ,
Karen K. Biron , Albert A. Smith , Charles A. Goss & Richard L. Miller (2000) Phosphorylation of
Ganciclovir Phosphonate by Cellular GMP Kinase Determines the Stereoselectivity of Anti-Human
Cytomegalovirus Activity, Nucleosides, Nucleotides and Nucleic Acids, 19:1-2, 341-356, DOI:
10.1080/15257770008033013
To link to this article: http://dx.doi.org/10.1080/15257770008033013
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