Synthesis of new liquid phase carriers for use in large scale oligonucleotide synthesis in solution

Article abstract of DOI:10.1016/S0040-4020(99)00058-7

The synthesis of multifunctional symmetric primary amines 4 and 8 and the covalent binding of 5'-O-dimethoxytrityl-deoxynucleoside derivatives to their amino groups (compound 5 and 9) is described. Different strategies for dedimethoxytritylation including the use of strong acidic ion exchangers or protic acids and modified silica gels and/or gel permeation chromatography are developed. The resulting liquid phase carriers 6 and 10 are suitable for large scale oligonucleotide synthesis in solution using phosphoamidites and gel permeation chromatography for fast isolation of intermediates.