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to 3-hydroxyflavone. This probe produced a strong fluores-
cence at 535 nm when treated with hydrazine, which indicated
a consequence of the ESIPT turn-on. The detection limit of
0.016 μM (0.512 ppb), which is lower than the EPA standard
(10 ppb). Importantly, we successfully demonstrated that this
probe could be used for intracellular hydrazine imaging in
living cells.
Acknowledgements We acknowledge the Joint Program for Fostering
Talents of National Natural Science Foundation of China and Henan
Province (U1304202), the National Natural Science Younth Foundation
of China (51302241), the Program for Science and Technology
Innovation Talents in Universities of Henan Province (15HASTIT002),
Technology Research and Development Program of Henan province
(122102210426, 152102210343), and Science and Technology Major
Project of Henan Province Educational Committee (14B150060,
15B150016), Technology Development Program of Xuchang
Municipality, and the Foundation for Excellent Youths of Xuchang
University for financial support.
19. Xiao L, Tu J, Sun S, Pei Z, Pei Y, Pang Y, Xu Y (2014) A fluores-
cent probe for hydrazine and its in vivo applications. RSC Adv 4:
41807–41811
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